Study of the Effect of Biosynthesized Silver Nanoparticles Using Coriandrum Sativum Leaf Extract on Some Types of Pathogenic Bacteria
Keywords:
Silver nanoparticles, Coriandrum sativum leaf extract, Staph, aureus, Ps. aeruginosaAbstract
The capacity to combat common infectious infections has been diminished by the overuse and frequent administration of conventional antibiotics, necessitating numerous medical procedures to prevent this condition. Therefore, one of the biggest threats to the effective treatment of bacterial diseases is the development of new antibacterial systems against drug-resistant microorganisms. Biocompatible nanomaterials provide potential strategies to prevent antimicrobial resistance to drugs, mainly by improving the therapeutic effect of existing antimicrobial drugs. Nanoparticles, also known as nanotechnology, have special physical and chemical characteristics, including a large surface area in relation to size, high reactivity, a functional structure, and a very small size that can be regulated, ranging from 1 to 100 nm. Coriander leaf extract was used to create silver nanoparticles, which were then thoroughly investigated using electrokinetic nanoparticles, atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), ultraviolet and visible spectroscopy (UV-Vis), and X-ray scattering (EDX). Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to assess the properties. Different concentrations were prepared for the nanoparticles: 500, 400, 300, 200, and 100 μg/ml. Their inhibitory efficacy was tested against isolates of Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa using the well diffusion method. The outcomes of the silver nanoparticle influence evaluated the efficiency of biosynthetic silver nanoparticles against bacterial isolates at five different concentrations: 500, 400, 300, 200, and 100 μg/ml. Most of the concentrations were successful in preventing the bacterial isolates from growing. The results showed that the effect of silver nanoparticles was better against the Gram-positive Staphylococcus aureus isolates than against the Gram-negative Pseudomonas aeruginosa isolates. The highest inhibitory efficacy was recorded with diameter rates of 22.00 mm for Staphylococcus aureus and 15.00 mm for Pseudomonas aeruginosa. The aqueous extract of coriander leaves has proven its ability to reduce and synthesize silver nanoparticles. When it comes to bacterial isolates, silver nanoparticles have shown the strongest inhibitory activity.
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Vivas, R., Barbosa, A. A. T., Dolabela, S. S., and Jain, S. (2019). Multidrug-resistant bacteria and alternative methods to control them: an overview. Microbial Drug Resistance, 25(6), 890-908.
Coulson, G. B., Johnson, B. K., Zheng, H., Colvin, C. J., Fillinger, R. J., Haiderer, E. R., ... and Abramovitch, R. B. (2017). Targeting Mycobacterium tuberculosis sensitivity to thiol stress at acidic pH kills the bacterium and potentiates antibiotics. Journal of Cell Chemical Biology, 24(8): 993-1004.
Huttner, A., Harbarth, S., Carlet, J., Cosgrove, S., Goossens, H., Holmes, A., ... and Pittet, D. (2013). Antimicrobial resistance: a global view from the 2013 World Healthcare-Associated Infections Forum. Journal of Antimicrobial resistance and infection control, 2(1): 1-13.
Gupta, A., Mumtaz, S., Li, C. H., Hussain, I., and Rotello, V. M. (2019). Combatting antibiotic-resistant bacteria using nanomaterials. Chemical Society Reviews, 48(2), 415-427.
Mulvey, M. R., and Simor, A. E. (2009). Antimicrobial resistance in hospitals: how concerned should we be? Canadian Medical Association Journal, 180(4), 408-415.
Hammood, I. A., Nijris, O. N., & Alwan, L. H. (2022). Comparing the effect of zinc oxide nanoparticles biosynthesized by Aloe barbdensis and chemically manufactured on Klebsiella pneumoniae isolated from the inflamed middle ear. International Journal of Health Sciences, 6(S2), 14976–14988.
Mofid O, Mobayen S, Wong WK.(2020). Adaptive terminal sliding mode control for attitude and position tracking control of quadrotor UAVs in the existence of external disturbance. IEEE access، 9:3428-3440.
Hamad, S. A., and Nijris, O. N. (2024). Effect of Silver Nanoparticles Biosynthesized by Chard Leaf Extract (Beta vulgaris) on the Levels of Cytokines IL-6, IL-10, and TNF-α in the Blood of Male Mice S. INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, 7(2), 1-22.
Ahmed, A. M. S. (2021). Determination of the antibacterial activity of synthesized copper nanoparticles against Staphylococcus aureus isolated from wound infection invivo and invitro. PhD thesis- College of Education for Pure Science- University of Tikri.
SAS J. Statistical Analysis System، Journal of Cary، North Carolina. USA، 2012; 45(5):1977-1983.
Huang CL, Huang HJ, Chen SH, Huang YS, Kao PC, Chau YFC, Chiang HP.(2021). Localized surface plasmon resonance enhanced by the light-scattering property of silver nanoparticles for improved luminescence of polymer light-emitting diodes. Journal of Industrial and Engineering Chemistry. 103: 283-291.
Heer ASK, Mansooria SM, Chamria N.(2017). Biosynthesis and characterization of Zno nanoparticles using ficus religiosa leaves extract. World Journal of Pharmaceutical. Res، 6(10): 818-826.
Hashim R, Oda AM, Fadhil I.(2019). Green synthesis of Silver Nanoparticles by Beta vulgaris (Chard) Extract: Characterization and Antibacterial Activity. Asian Journal of Chemistry، 31(8):1881-1884.
Abdel-Wahhab MA, Ahmed H, El-Nekeety AA, Abdel-Aziem SH, Shara HA, Abdelaziz M, Sallam MF, Mannaa FA.(2020). Chenopodium murale essential oil alleviates the genotoxicity and oxidative stress of silver nanoparticles in the rat kidney. Egyptian Journal of Chemistry، 63(7):2631-2646.
Singh OV, editor.(2015). Bio-nanoparticles: biosynthesis and sustainable biotechnological implications. John Wiley and Sons. 2015 Jun 22.
Huq، MA.(2020). Green synthesis of silver nanoparticles using Pseudoduganella eburnea MAHUQ-39 and their antimicrobial mechanisms investigation against drug-resistant human pathogens. International journal of molecular sciences، 21(4): 1510.
Ami D, Mereghetti , Natalello A.(2022). Contribution of infrared spectroscopy to the understanding of amyloid protein aggregation in complex systems. Frontiers in Molecular Biosciences، Apr 8; 9: 822852.
Sampaio S, Viana JC.(2018). Production of silver nanoparticles by green synthesis using artichoke (Cynara scolymus L.) aqueous extract and measurement of their electrical conductivity. Advances in Natural Sciences: Nanoscience and Nanotechnology، 9(4):045002.
Abas Hamad, S. (2024). Effect of Silver Nanoparticles Biosynthesized by Chard Leaf Extract (Beta vulgaris) on the Levels of IgM and IgG Immunoglobulins in the Blood of Male Mice. International Journal of Medical Sciences, 7(1), 56-76.
Garcidueñas-Piña C, Tirado-Fuentes C, Ruiz-Pérez , Valerio-García RC, Morales-Domínguez JF.(2023). Silver Nanoparticles Synthesized with Extracts of Leaves of Raphanus sativus L, Beta vulgaris L, and Ocimum Basilicum and Its Application in Seed Disinfection. Nanomaterials and Nanotechnology، Aug 11,2023.
Saleh, Riyam Fares, Maarouf, Muhammad Nazir, Hamza, Haidar Musa.(2020). Determining the inhibitory efficacy of silver nanoparticles against some antibiotic-resistant multi pathogenic bacteria, Research Gate, pp. 1-12.
Bjarnsholt, T., Kirketerp‐Møller, K. L. A. U. S., Kristiansen, S., Phipps, R., Nielsen, A. K., Jensen, P. O., Hoiby, N. and Givskov, M.(2007). Silver against Pseudomonas aeruginosa biofilms. Apmis, 115(8), 921-928.
Kathiresan, G., and Kanimozhi, N. A. (2019). Silver Nanoparticle: A Bactericidal Agent for Pathogenic Poultry Bacteria. International Journal of Recent Technology and Engineering (IJRTE). ISSN: 2277-3878, Volume-7 Issue-6S2, April 2019.
Nimma, D., Aarif, M., Pokhriyal, S., Murugan, R., Rao, V. S., & Bala, B. K. (2024, December). Artificial Intelligence Strategies for Optimizing Native Advertising with Deep Learning. In 2024 International Conference on Artificial Intelligence and Quantum Computation-Based Sensor Application (ICAIQSA) (pp. 1-6). IEEE.
Dash, C., Ansari, M. S. A., Kaur, C., El-Ebiary, Y. A. B., Algani, Y. M. A., & Bala, B. K. (2025, March). Cloud computing visualization for resources allocation in distribution systems. In AIP Conference Proceedings (Vol. 3137, No. 1). AIP Publishing.
Kumar, A. P., Fatma, G., Sarwar, S., & Punithaasree, K. S. (2025, January). Adaptive Learning Systems for English Language Education based on AI-Driven System. In 2025 International Conference on Intelligent Systems and Computational Networks (ICISCN) (pp. 1-5). IEEE.
Elkady, G., Sayed, A., Priya, S., Nagarjuna, B., Haralayya, B., & Aarif, M. (2024). An Empirical Investigation into the Role of Industry 4.0 Tools in Realizing Sustainable Development Goals with Reference to Fast Moving Consumer Foods Industry. In Advanced Technologies for Realizing Sustainable Development Goals: 5G, AI, Big Data, Blockchain, and Industry 4.0 Application (pp. 193-203). Bentham Science Publishers.
Kaur, C., Al Ansari, M. S., Rana, N., Haralayya, B., Rajkumari, Y., & Gayathri, K. C. (2024). A Study Analyzing the Major Determinants of Implementing Internet of Things (IoT) Tools in Delivering Better Healthcare Services Using Regression Analysis. In Advanced Technologies for Realizing Sustainable Development Goals: 5G, AI, Big Data, Blockchain, and Industry 4.0 Application (pp. 270-282). Bentham Science Publishers.
Alijoyo, F. A., Prabha, B., Aarif, M., Fatma, G., & Rao, V. S. (2024, July). Blockchain-Based Secure Data Sharing Algorithms for Cognitive Decision Management. In 2024 International Conference on Electrical, Computer and Energy Technologies (ICECET (pp. 1-6). IEEE.
Elkady, G., Sayed, A., Mukherjee, R., Lavanya, D., Banerjee, D., & Aarif, M. (2024). A Critical Investigation into the Impact of Big Data in the Food Supply Chain for Realizing Sustainable Development Goals in Emerging Economies. In Advanced Technologies for Realizing Sustainable Development Goals: 5G, AI, Big Data, Blockchain, and Industry 4.0 Application (pp. 204-214). Bentham Science Publishers.
Praveena, K., Misba, M., Kaur, C., Al Ansari, M. S., Vuyyuru, V. A., & Muthuperumal, S. (2024, July). Hybrid MLP-GRU Federated Learning Framework for Industrial Predictive Maintenance. In 2024 Third International Conference on Electrical, Electronics, Information and Communication Technologies (ICEEICT) (pp. 1-8). IEEE.
Orosoo, M., Rajkumari, Y., Ramesh, K., Fatma, G., Nagabhaskar, M., Gopi, A., & Rengarajan, M. (2024). Enhancing English Learning Environments Through Real-Time Emotion Detection and Sentiment Analysis. International Journal of Advanced Computer Science & Applications, 15(7).
Tripathi, M. A., Goswami, I., Haralayya, B., Roja, M. P., Aarif, M., & Kumar, D. (2024). The Role of Big Data Analytics as a Critical Roadmap for Realizing Green Innovation and Competitive Edge and Ecological Performance for Realizing Sustainable Goals. In Advanced Technologies for Realizing Sustainable Development Goals: 5G, AI, Big Data, Blockchain, and Industry 4.0 Application (pp. 260-269). Bentham Science Publishers.
Kaur, C., Al Ansari, M. S., Dwivedi, V. K., & Suganthi, D. (2024). Implementation of a Neuro‐Fuzzy‐Based Classifier for the Detection of Types 1 and 2 Diabetes. Advances in Fuzzy‐Based Internet of Medical Things (IoMT), 163-178.
Yousuf, M. M., Shaheen, N., Kheri, N. A., & Fatma, G. (2023). Exploring Effective Classroom Management Techniques in English Teaching. International Journal on Recent and Innovation Trends in Computing and Communication, 11(11), 382-393.
Tripathi, M. A., Singh, S. V., Rajkumari, Y., Geethanjali, N., Kumar, D., & Aarif, M. (2024). The Role of 5G in Creating Smart Cities for Achieving Sustainable Goals: Analyzing the Opportunities and Challenges through the MANOVA Approach. Advanced Technologies for Realizing Sustainable Development Goals: 5G, AI, Big Data, Blockchain, and Industry 4.0 Application, 77-86.
Kaur, C., Al Ansari, M. S., Dwivedi, V. K., & Suganthi, D. (2024). An Intelligent IoT‐Based Healthcare System Using Fuzzy Neural Networks. Advances in Fuzzy‐Based Internet of Medical Things (IoMT), 121-133.
Rajeshkumar, S., and Malarkodi, C. (2014). In vitro antibacterial activity and mechanism of silver nanoparticles against foodborne pathogens. Bioinorganic chemistry and applications, 10P.
Zaidi, S., Misba, L., and Khan, A. U. (2017). Nano-therapeutics: a revolution in infection control in post-antibiotic era. Nanomedicine: Nanotechnology, Biology and Medicine, 13(7), 2281-2301.
Marambio-Jones, C., and Hoek, E. (2010). A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. Journal of nanoparticle research, 12(5), 1531-1551.
Matsumura, Y., Yoshikata, K., Kunisaki, S. I., and Tsuchido, T. (2003). Mode of bactericidal action of silver zeolite and its comparison with that of silver nitrate. Applied and environmental microbiology, 69(7), 4278-4281.
Gogoi, S. K., Gopinath, P., Paul, A., Ramesh, A., Ghosh, S. S., and Chattopadhyay, A. (2006). Green fluorescent protein-expressing Escherichia coli as a model system for investigating the antimicrobial activities of silver nanoparticles. Langmuir, 22(22), 9322-9328.
Khitam, S. S., Alhtheal, E. D., and Azhar, J. B. (2018). Effect of Zinc Oxide nanoparticles preparation from Zinc Sulphate (ZnSo4) against gram-negative or gram-positive microorganisms in vitro. The Iraqi Journal of Veterinary Medicine, 42(1), 18-22.
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