Dose Response Curve of Acetylecholine, Its Modification by Water Extract of Allium Sativum and Estimation of PD2 Value Taking Atropine as A Standard Drugs
Keywords:
Allium sativum (garlic), dose-response curves (DRC), acetylcholine, atropine, PD₂ value, Antagonist activity, Chicken ileum, anticholinergicAbstract
This research aims to study how the water extract of Allium sativum (garlic) affects muscle contractions caused by acetylcholine (ACh) in isolated chicken intestines, using atropine as a comparison drug. The experiment included documenting dose-response curves (DRC) of acetylcholine both alone and in conjunction with atropine (a recognized competitive muscarinic antagonist) and Allium sativum aqueous extract (0.5 mL). The contractile responses were documented in arbitrary units, and PD₂ values were computed to evaluate antagonist potency. The DRC of ACh alone displayed a characteristic sigmoidal pattern with escalating muscular contraction, resulting in a PD₂ value of 5.075. The addition of atropine resulted in a rightward shift of the DRC, yielding a PD₂ value of 5.375, so confirming its potent competitive antagonism. Correspondingly, the DRC with Allium sativum extract exhibited a rightward shift, yielding a PD₂ value of 5.125, indicating considerable anticholinergic action. The findings suggest that Allium sativum contains active compounds that might reduce the effects of ACh on muscarinic receptors, but they are not as strong as atropine. The research supports the traditional use of garlic for easing stomach cramps and suggests it could be a natural source of substances that block muscarinic activity. Additional research is necessary to identify the precise phytoconstituents responsible for the reported effects and to examine their therapeutic significance. There was a very little evidences are found of anticholinergic activity towards the different concentration of Ach on chicken ileum and its modifications in response to water extract of Allium sativum. This research aims to study how the water extract of Allium sativum (garlic) affects muscle contractions caused by acetylcholine (ACh) on isolated chicken intestine. Here Atropine was applied as model standard drug to compare the ant cholinergic effect of garlic water extract.
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