Isolation and Characterization of Bacteria that Promote Plant Growth (PGPB) from the Rhizosphere and Endosphere of Ficus Carica
Keywords:
Ficus carica, PGPB, IAA, GA, Rhizospheric and Endospheric microorganismsAbstract
In order to promote sustainable agriculture, environmental preservation, and biotechnological developments, it is crucial to isolate and characterize rhizospheric and endospheric bacteria. The fruit of Ficus carica, commonly known as fig, belongs to the Moraceae family. The Ficus genus contains a diverse range of phytochemicals, including phenolics, polyphenols, flavonoids, tannins, anthocyanins, coumarins, volatile compounds, glycosides, saponins, carotenoids, alkaloids, triterpenoids, and vitamins. Due to this rich phytochemical profile, fig fruit holds significant value in the medical field. The goal of the study was to separate, screen, and describe (PGPB) bacteria that promote plant growth from the rhizosphere and endosphere of Ficus carica. These bacteria included those that produce phosphate solubilizers, gibberellic acid (GA), and indole acetic acid (IAA). Samples of rhizospheric soil and Ficus carica roots were gathered in order to isolate microorganisms. The study's goal is to determine whether bacterial isolates can create gibberellic acid (GA), indole acetic acid (IAA), and solubilize phosphate. We also characterized the isolates based on their cultural, morphological, and biochemical traits. Among the 15 isolates collected, five in particular showed a significant capacity for gibberellic acid (GA) synthesis, making them potential candidates for further study in agricultural applications to boost crop growth. Five isolates (IS1, IS7, IS8, IS9, and IS15) showed phosphate solubilization, suggesting their ability to transform insoluble forms of phosphate into plant-bioavailable forms. Interestingly, five of these phosphate-solubilizing isolates (IS1, IS7, IS8, IS9, and IS15) may also generate Indole Acetic Acid (IAA) and Gibberellic Acid (GA), making them extremely intriguing. While all isolates show promise, IS1 and IS15 appear to be the most adaptable in terms of promoting overall plant growth due to their high amounts of Indole Acetic Acid (IAA), Gibberellic Acid (GA), and phosphate solubility. The study's findings have the potential to assist a wide range of sectors by promoting more ecological and economically viable methods
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