Preliminary Phytochemical Screening, Quantitative Phytochemical Estimation & Crocetin Characterization From Nyctanthes Arbor-Tristis Flower Extract.
DOI:
https://doi.org/10.63682/jns.v14i32S.9911Keywords:
Nyctanthes arbor-tristis, Phytochemical Quantification, In-vitro Methods, Antioxidant activity, Spectroscopic Characterization, NMR & MASSAbstract
Nyctanthes arbor-tristis Linn., a traditionally important medicinal plant, was investigated for its phytochemical composition and in vitro pharmacological potential. The plant extract was prepared using different solvents and subjected to preliminary qualitative and quantitative phytochemical analysis. Key bioactive constituents such as phenolics, flavonoids, and terpenoids were quantified using standard spectrophotometry methods. The extract exhibited a high content of these phytoconstituents, which are known for their therapeutic significance. In vitro studies demonstrated notable antioxidant, anti-inflammatory, and antimicrobial activities, supporting the correlation between phytochemical composition and biological efficacy. Furthermore, crocetin, an important carotenoid compound, was successfully identified and characterized using techniques such as UV–Visible spectroscopy, FT-IR, NMR, and mass spectrometry. The results scientifically validate the traditional uses of Nyctanthes arbor-tristis and suggest its potential as a natural source for the development of effective therapeutic agents. The findings of this study scientifically validate the traditional medicinal claims of Nyctanthes arbor-tristis and suggest its potential as a natural source of therapeutic agents for the management of oxidative stress-related and inflammatory conditions
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