Extraction And Characterization Of Collagen And Development Of Value - Added Food Product By Blending With Fresh Cheese

Authors

  • Aurobinda Das
  • Yashaswi Nayak
  • Supriya Dash

Keywords:

Fish viscera, hydrolysate, Labeorohita, protease enzymes, bioactive compounds

Abstract

Despite its reputation as a useless byproduct, fish viscera has been shown to be rich in useful proteins, peptides, and amino acids. In this research, we explore the potential of Labeorohita fish visceral hydrolysate in the food, pharmaceutical, and other product manufacturing sectors. Enzymatic hydrolysis with alcalase and protease enzymes was used to produce the hydrolysate. Protein and antioxidant activity were both elevated in the resultant hydrolysate. The hydrolysate was used into food products as a functional component to improve their nutritional profile. It was also discovered that the hydrolysate possessed antibacterial qualities, meaning it might be used as a natural preservative in foods. Hydrolysate was evaluated for its potential as a wound healer and anticoagulant by the pharmaceutical sector. The findings suggested that the hydrolysate might be utilised as a natural anticoagulant and also had a substantial impact on wound healing. Hydrolysate of Labeorohita fish viscera has promising uses in the food, pharmaceutical, and other industries due to its high concentration of bioactive chemicals.

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References

Abdelhedi O, Nasri R, Mora L, Jridi M, Toldrá F, Nasri M (2018) In silico analysis and molecular docking study of angiotensin I-converting enzyme inhibitory peptides from smooth-hound viscera protein hydrolysates fractionated by ultrafiltration. Food Chem 239:453–463. https://doi.org/10.1016/j.foodchem.2017.06.112

Abdul-Hamid A, Bakar J, Bee GH (2002) Nutritional quality of spray dried protein hydrolysate from Black Tilapia (Oreochromis mossambicus). Food Chem 78(1):69–74. https://doi.org/10.1016/S0308-8146(01)00380-6

Abejón R, Abejón A, Belleville MP, Sánchez-Marcano J, Garea A, Irabien Á (2016) Multiobjective Optimization of Membrane Networks for Fractionation of Protein Hydrolysate from Fish By-Products. In: Kravanja Z, Bogataj M (eds) Computer Aided Chemical Engineering. Elsevier, Amsterdam

Abejón R, Belleville MP, Sanchez-Marcano J, Garea A, Irabien A (2018) Optimal design of industrial scale continuous process for fractionation by membrane technologies of protein hydrolysate derived from fish wastes. Sep Purif Technol 197:137–146. https://doi.org/10.1016/j.seppur.2017.12.057

Aliani M, Eskin MNA (2017) Bitterness: perception, chemistry and food processing. Wiley, New Jersey

Al-Mansour HE, Al-Busairi BH, Baker CGJ (2011) Energy consumption of a pilot-scale spray dryer. Dry Technol 29(16):1901–1910. https://doi.org/10.1080/07373937.2011.595563

Alvares TS, Conte-Junior CA, Pierucci AP, de Oliveira GV, Cordeiro EM (2018) Acute effect of fish protein hydrolysate supplementation on vascular function in healthy individuals. Journal of Functional Foods 46:250–255. https://doi.org/10.1016/j.jff.2018.04.066

ASHRAE (1999) ASHRAE handbook. American Society of Heating, Refrigerating and Air Conditioning Engineers, Atlanta

Aubes-Dufau I, Capdevielle J, Seris JL, Combes D (1995) Bitter peptide from hemoglobin hydrolysate: isolation and characterization. FEBS Lett 364(2):115–119

Baker CGJ, McKenzie KA (2005) Energy consumption of industrial spray dryers. Dryi Technol 23(1–2):365–386. https://doi.org/10.1081/DRT-200047665

Bikov VP, Ionas GP, Golovkova GN, Didenko AP, Akulin VN, Perova LI, Odincov AB, Konstantinova LL, Dvinin UF, Christoferzen GS, Borisova LP (1998) Book of chemical content and technological properties of seawater and oceanic fishes. VNIRO Press, Moscow

Blenford DE (1994) Protein hyddrolysates: functionalities and uses in nutritional products. Int Food Ingr 3:45–49

Bourseau P, Vandanjon L, Jaouen P, Chaplain-Derouiniot M, Massé A, Guérard F, Chabeaud A, Fouchereau-Pérond M, Le Gal Y, Ravallec-Plé L, Bergé J-P, Picot L, Piot J-M, Batista I, Thorkelsson G, Delannoy C, Jakobsen G, Johansson I (2009) Fractionation of fish protein hydrolysates by ultrafiltration and nanofiltration: impact on peptidic populations. Desalination 244(1):303–320. https://doi.org/10.1016/j.desal.2008.05.026

Chae HJ, In MJ, Kim MH (1998) Process development for the enzymatic hydrolysis of food protein: effects of pre-treatment and post-treatments on degree of hydrolysis and other product characteristics. BiotechnolBioproc E 3(1):35–39. https://doi.org/10.1007/BF02932481

Chakraborty PK, Madhavan P (1977) A pilot scale set up for the manufacture of fish hydrolysate. Fish Technol 14(2):159

Chalamaiah M, Dinesh Kumar B, Hemalatha R, Jyothirmayi T (2012) Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: A review. Food Chem 135(4):3020–3038. https://doi.org/10.1016/j.foodchem.2012.06.100

Cui H (1996) The influence of degree of hydrolysis on the structural and functional properties of fish protein peptic hydrolysates. Masters thesis. University of Washington, Seattle

Damodaran S, Parkin KL, Fennema OR (2008) Fennema’s Food Chemistry. CRC Press, Boca Raton

Danilov OI, Leontchik BI (1986) Energy Economic in Thermal Drying. Energoatomizdat, Moscow

Di Pasquale MG (2008) Amino acids and proteins for the athlete: the anabolic edge. CRC Press, Boca Raton

Elavarasan K, Shamasundar BA, Badii F, Howell N (2016) Angiotensin I-converting enzyme (ACE) inhibitory activity and structural properties of oven- and freeze-dried protein hydrolysate from fresh water fish (Cirrhinusmrigala). Food Chem 206:210–216. https://doi.org/10.1016/j.foodchem.2016.03.047

European Comission (2012) https://ec.europa.eu/fisheries/cfp/aquaculture/species_en. Accessed 26 Sept 2018

Gonzalez-Tello P, Camacho F, Jurado E, Paez MP, Guadix EM (1994) Enzymatic hydrolysis of whey proteins: i. Kinetic models. BiotechnolBioeng 44(4):523–528. https://doi.org/10.1002/bit.260440415

Halim NRA, Azlan A, Yusof HM, Sarbon NM (2018) Antioxidant and anticancer activities of enzymatic eel (monopterussp) protein hydrolysate as influenced by different molecular weight. Biocatal Agric Biotechnol 16:10–16. https://doi.org/10.1016/j.bcab.2018.06.006

He S, Franco C, Zhang W (2013) Functions, applications and production of protein hydrolysates from fish processing co-products (FPCP). Food Res Int 50(1):289–297. https://doi.org/10.1016/j.foodres.2012.10.031

Himonides AT, Taylor AKD, Morris AJ (2011) Enzymatic Hydrolysis of Fish Frames Using Pilot Plant Scale Systems. Food Nutr Sci 2:586–593

Hoeling A, Volkov V (2015) Proteins from by-products - innovative components in sustainable industrial production. Proceedings of KGTU 38:83–92

Huang S, Vignolles ML, Chen XD, Le LY, Jan G, Schuck P, Jeantet R (2017) Spray drying of probiotics and other food-grade bacteria: a review. Trends Food Sci Tech 63:1–17. https://doi.org/10.1016/j.tifs.2017.02.007

Jaswal AS (1990) Amino Acid Hydrolysate from Crab Processing Waste. J Food Sci 55(2):379–380. https://doi.org/10.1111/j.1365-2621.1990.tb06768.x

Jenkelunas PJ, Li-Chan ECY (2018) Production and assessment of Pacific hake (Merluccius productus) hydrolysates as cryoprotectants for frozen fish mince. Food Chem 239:535–543. https://doi.org/10.1016/j.foodchem.2017.06.148

John TG (1993) Non-bitter protein hydrolyzates. US Patent 5266685, USA

Juming THF, Shen GQ (2003) Drum Drying. In: Encyclopedia of agricultural, food, and biological engineering. Marcel Dekker, Inc., New York, pp 211–214. https://doi.org/10.1081/e-eafe 120007091

Kinsella JE, Melachouris N (1976) Functional properties of proteins in foods: a survey. Crit Rev Food Sci 7(3):219–280. https://doi.org/10.1080/10408397609527208

Kristinsson HG (1998) Reaction kinetics, biochemical and functional properties of salmon muscle proteins hydrolyzed by different alkaline proteases. Masters thesis. University of Washington, Seattle

Kristinsson HG, Rasco BA (2000) Fish Protein Hydrolysates: production, Biochemical, and Functional Properties. Critical Rev Food Sci 40(1):43–81. https://doi.org/10.1080/10408690091189266

Kuehler CA, Stine CM (1974) Effect of enzymatic hydrolysis on some functional properties of whey protein. J Food Sci 39(2):379–382. https://doi.org/10.1111/j.1365-2621.1974.tb02899.x

Lahl WJ, Braun SD (1994) Enzymatic production of protein hydrolysates for food use. Food Sci 48:68–71

Linder M, Fanni J, Parmentier M, Sergent M, Phan-Tan-Luu R (1995) Protein recovery from veal bones by enzymatic hydrolysis. J Food Sci 60(5):949–952. https://doi.org/10.1111/j.1365-2621.1995.tb06268.x

Loffler A (1986) Proteolytic enzymes: sources and applications. Food Technol-Chicago 40(1):63

Loosen P, Bressollier P, Julien R, Pejoan CH, Verneuil BG (1994) Method for preparing an enzymatic hydrolysate. United States Patent 5356637

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Published

2025-07-05

How to Cite

1.
Das A, Nayak Y, Dash S. Extraction And Characterization Of Collagen And Development Of Value - Added Food Product By Blending With Fresh Cheese. J Neonatal Surg [Internet]. 2025Jul.5 [cited 2025Jul.19];14(32S):3774-86. Available from: https://jneonatalsurg.com/index.php/jns/article/view/8011