Detection of Metallo-Beta-Lactamase (MBL) Producing Pseudomonas aeruginosa in a Tertiary Care Hospital, Udaipur, Rajasthan
Keywords:
Pseudomonas aeruginosa, antimicrobial resistance, Carbapenem, Metallo-β-LactamaseAbstract
Introduction: P. aeruginosa is a prevalent pathogenic agent in neonatal critical care units due to its widespread distribution, strong preference for wet environments, and capacity to thrive in a variety of settings. Predisposing factor with considerable morbidity and fatality rates in Pseudomonas septicemia are invasive diagnostic and therapeutic procedures, and indiscriminate antibiotic usage Pseudomonas aeruginosa has been found to cause a mixture of infections in clinical practice: like chronic Cystic fibrosis (CF) lung infection, septicemia in burn patients, surgical wound infection, urinary tract infection, corneal ulceration and Infrequently, P. aeruginosa can colonize human body sites, with a partiality for moist areas, such as the perineum, axilla, ear, nasal mucosa, throat, as well as stools.
Aims & objectives: Isolation and identification of Pseudomonas species. To find out prevalence of Pseudomonas aeruginosa from various clinical samples. To detect Metallo beta lactamase in Pseudomonas species isolated from various clinical samples.
Materials and methods: Various clinical samples like Urine, Endotracheal, Tracheostomy, Blood, Pus, Sputum, and CSF were collected by aseptic technique in sterile container except blood which is collected in blood culture bottle. Antimicrobial susceptibility testing will be done on Mueller Hinton agar (MHA) according to CLSI guidelines for Kirby Bauer disc diffusion test. After 24 hours of incubation zone of inhibition were measured. Phenotypic metallo-beta-lactamase also detected as per standard guidelines.
Results and Observation: In our research study we have analyzed 1000 various clinical samples i.e Urine, ET, TT Blood, Pus, Sputum, and CSF and isolate 400 samples of pseudomonas species. Out of 400 Pseudomonas species 300 were identified as Pseudomonas aeruginosa. The prevalence of Pseudomonas aeruginosa was 30 percent. Detection of Metalloid-β-lactamases (MBLs) producing Pseudomonas aeruginosa. In our study we have noted that maximum resistance was found in Imipenem 112 (37.33%). Out of 112 imipinem resistant Pseudomonas aeruginosa, 40 (35.71%) were detected as a MBL producer. Phonotypical detection of Metallo-β-lactamases.
Discussion and Conclusion: The emergence of carbapenem resistance reflects a threat limiting treatment choices and suggests the need for on-going epidemiological and antimicrobial susceptibility studies and longitudinal surveillance of antibiotic prescription. Increase in antibacterial resistance in P. aeruginosa is a cause of concern. So, continuous monitoring of bacterial resistance trends should be done and therapy should be based on antibacterial susceptibility results...
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