Formulation And Evaluation of plant-based Hydrogel for Antimicrobial Activity
DOI:
https://doi.org/10.52783/jns.v14.3619Keywords:
Hydrogel, Chitosan, Phytochemical screening, Anti-microbial activityAbstract
The study was aimed to develop and evaluate a plant-based hydrogel incorporating chitosan polymer for its in-vitro antibacterial activity. The hydrogel was prepared by using chitosan polymer, methyl paraben, propyl paraben, glycerin, acetic acid, triethanolamine, moringa oleifera leaf extract and distilled water. Triethanolamine was gradually added drop by drop to maintain the pH of skin. The hydrogel formulations were assessed based on various parameters, including physical appearance, homogeneity, viscosity, extrudability, pH, spreadability, moisture content and antimicrobial efficacy. The formulated gel was dark brown, homogenous and pH ranges from 6.24 to 6.29 which is close to the skin pH. Formulation F4 showed acceptable rheological property with applicable spreadability, extrudability, moisture content and in- vitro antibacterial activity. The phytochemical screening of methanolic extract revealed the presence of various secondary metabolites such as flavonoids, alkaloids, glycosides, terpenoids, saponins, phenols and tannins. Antimicrobial activity was tested using the agar well diffusion method. The m. oleifera plant was recognized as a rich source of natural antimicrobial agents due to its phytochemical properties. The antimicrobial activity of the test sample was quantified by measuring the diameter of the inhibition zone in millimeters. The formulation F4 shows highest inhibition zone of 17mm at the highest concentration (100mg/ml). Antimicrobial screening revealed that the crude methanolic extract exhibited antimicrobial activity against Staphylococcus epidermidis
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Mosihuzzaman M, Choudhary M. Protocols on safety, efficacy, standardization, and documentation of herbal medicine (IUPAC Technical Report). Pure and Applied Chemistry. 2008;80(10): 2195-2230. https://doi.org/10.1351/pac200880102195
Modak, Manisha, et al. "Indian herbs and herbal drugs used for the treatment of diabetes." Journal of clinical biochemistry and nutrition 40.3 (2007): 163-173.
Muyumba, N. W., et al. "Quality control of herbal drugs and preparations: The methods of analysis, their relevance and applications." Talanta Open 4 (2021): 100070.
Wani MS, Parakh SR, Dehghan MH, Polshettiwar SA, Chopade VV, Chepurwar S. Herbal medicine and its standardization. Pharmaceutical Reviews. 2007;5(6):1-9.
Fu J, Yang F, Guo Z. The chitosan hydrogels: From structure to function. New Journal of Chemistry.2018;42(21):17162-80.
Singla, A. K., and M. Chawla. "Chitosan: Some pharmaceutical and biological aspects‐an update." Journal of pharmacy and pharmacology 53, no. 8 (2001): 1047-1067.
Öztürk B. Preparation and characterization of crosslinked chitosan based hydrogels (Master's thesis, Dokuz Eylul Universitesi (Turkey)).
Berger J, Reist M, Mayer JM, Felt O, Peppas NA, Gurny RJ. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. European journal of pharmaceutics and biopharmaceutics. 2004 Jan 1;57(1):19-34.
De Azevedo EP. Chitosan hydrogels for drug delivery and tissue engineering applications. International Journal of Pharmacy and Pharmaceutical Sciences. 2015;7:8-14.
Venkatesan J, Jayakumar R, Mohandas A, Bhatnagar I, Kim SK. Antimicrobial activity of chitosan-carbon nanotube hydrogels. Materials. 2014 May 19;7(5):3946-55.
Ullah F, Othman MB, Javed F, Ahmad Z, Akil HM. Classification, processing and application of hydrogels: A review. Materials Science and Engineering: C. 2015 Dec 1;57:414-33.
Thirupathi K, Raorane CJ, Ramkumar V, Ulagesan S, Santhamoorthy M, Raj V, Krishnakumar GS, Phan TT, Kim SC. Update on chitosan-based hydrogels: Preparation, characterization, and its antimicrobial and antibiofilm applications. Gels. 2022 Dec 30;9(1):35.
Khan S, Maryam L, Gulzar A, Mansoor MA, Iqbal M. smart and active hydrogels in biotechnology—synthetic techniques and applications. Journal of Materials Science. 2024 Sep 11:1-23.
Khandan A, Jazayeri H, Fahmy MD, Razavi M. Hydrogels: Types, structure, properties, and applications. Biomat Tiss Eng. 2017 Oct 3;4(27):143-69.
Islam Z, Islam SR, Hossen F, Mahtab-ul-Islam K, Hasan MR, Karim R. Moringa oleifera is a prominent source of nutrients with potential health benefits. International Journal of Food Science. 2021;2021(1):6627265.
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