Aloe Vera and Pear Extract Serum: A Herbal Approach to Intertrigo Management
DOI:
https://doi.org/10.63682/jns.v14i11S.3123Keywords:
Intertrigo, Aloe vera, Pear extract, Herbal serum, HPTLC, Analytical validation, Oleanolic acid, Aloe emodinAbstract
Background: Intertrigo is an inflammatory skin condition that occurs in newborns, infants and adults, typically affecting skin folds (flexural areas) such as the neck, armpits, groin, and under the chin. It results from a combination of moisture, friction, and microbial overgrowth, leading to redness, irritation, and maceration of the skin. Conventional treatments primarily focus on symptom management, highlighting the need for safer, plant-based alternatives. This study aimed to develop and validate a novel herbal serum containing Aloe vera (Aloe barbadensis) and pear (Pyrus communis) extracts for intertrigo treatment, with an emphasis on formulation optimization and analytical method validation.
Methods: The formulation was developed using Carbopol as a gelling agent to ensure optimal viscosity and stability. High-Performance Thin-Layer Chromatography (HPTLC) was employed for qualitative and quantitative analysis of Aloe emodin (Aloe vera) and Oleanolic acid (pear extract). Analytical method validation was performed following ICH Q2(R1) guidelines, evaluating parameters such as linearity, precision, accuracy, robustness, limit of detection (LOD), and limit of quantification (LOQ).
Results: The formulated serum exhibited excellent spreadability, pH compatibility, and homogeneity. HPTLC analysis demonstrated well-defined bands for Aloe emodin and Oleanolic acid at optimized Rf values. The method showed linearity (500–1500 ng/spot, R² > 0.999), precision (%RSD < 2%), and accuracy (98–102% recovery). The established LOD and LOQ values confirmed high sensitivity, ensuring reliable quantification of the bioactive markers. Robustness studies demonstrated the stability of the analytical method under minor variations.
Conclusion: The developed herbal serum demonstrated promising physicochemical properties and stability, while the validated HPTLC method provided accurate and reproducible quantification of Aloe emodin and Oleanolic acid. These findings support the potential of this novel herbal formulation as a natural therapeutic option for intertrigo.
Downloads
Metrics
References
Vakharia P, William J, Krusinski P, Vinson R, Franklin F, Selden S. Intertrigo. 2020 Nov 13.
Budiasih S, Masyitah I, Jiyauddin K, Kaleemullah M, Samer AD, Fadli AM, Eddy Y. Formulation and Characterization of Cosmetic Serum Containing Argan Oil as Moisturizing Agent. In Proceedings of the BROMO Conference, Surabaya, Indonesia 2018 Jul (pp. 11-12).
Ojha S, Chadha H, Aggarwal B. Formulation and Evaluation of Face Serum Containing Bee. World J Pharm Res. 2019 Feb; 8:1100-5.
Aggnihotri S. Formulation and development of botanicals-based herbal serum. Pharmaspire. 2021 Oct; 13:211-7.
Zagorska-Dziok M, Furman-Toczek D, Dudra-Jastrzebska M, Zygo K, Stanisławek A, Kapka-Skrzypczak L. Evaluation of clinical effectiveness of Aloe vera -a review. Journal of Pre-Clinical and Clinical Research. 2017;11(1).
Sharma P, Kharkwal AC, Kharkwal H, Abdin MZ, Varma A. a review on pharmacological properties of Aloe vera. Int J Pharm Sci Rev Res. 2014 Nov;29(2):31-7.
Pandey A., Shweta S., Aloe Vera: A Systematic Review of its Industrial and Ethno-Medicinal Efficacy, International Journal of Pharmaceutical Research & Allied Sciences, 2016, 5(1): 21-33
Ibrahim RM, Hammoudi ZM. Phytochemistry and pharmacological activity of pear (Pyrus communis linn): a review. Plant Archives, 20, 2, 2020;7820-7828.
Li X, Wang T, Zhou B, Gao W, Cao J, Huang L. Chemical composition and antioxidant and anti-inflammatory potential of peels and flesh from 10 different pear varieties (Pyrus spp.). Food chemistry. 2014 Jun 1; 152:531-8.
Ayeleso TB, Matumba MG, Mukwevho E. Oleanolic acid and its derivatives: biological activities and therapeutic potential in chronic diseases. Molecules. 2017 Nov 13;22(11):1915
Wolska KI, Grudniak AM, Fiecek B, Kraczkiewicz-Dowjat A, Kurek A. Antibacterial activity of oleanolic and ursolic acids and their derivatives. Central European Journal of Biology. 2010 Oct;5(5):543-53.
Blanco-Cabra N, Vega-Granados K, Moya-Andérico L, Vukomanovic M, Parra A, Alvarez de Cienfuegos L, Torrents E. Novel oleanolic and maslinic acid derivatives as a promising treatment against bacterial biofilm in nosocomial infections: An in vitro and in vivo study. ACS Infectious Diseases. 2019 Jul 3;5(9):1581
Jesus JA, Lago JH, Laurenti MD, Yamamoto ES, Passero LF. Antimicrobial activity of oleanolic and ursolic acids: an update. Evidence-Based Complementary and Alternative Medicine. 2015 Oct;2015.
Dong X, Zeng Y, Liu Y, You L, Yin X, Fu J, Ni J. Aloe-emodin: A review of its pharmacology, toxicity, and pharmacokinetics. Phytother Res. 2020 Feb;34(2):270-281. doi: 10.1002/ptr.6532. Epub 2019 Nov 3. PMID: 31680350.
ICH. International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceutical for Human Use (ICH) guidelines Q2 (R2). Validation of analytical procedure. Geneva, Switzerland. 2022.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
