Evaluation of Phytochemical Constituents, Antioxidant & Anti-Diabetic Activity of Seaweed Turbinaria Decurrens Collected from Gulf of Mannar, Tamil Nadu, India
DOI:
https://doi.org/10.52783/jns.v14.2039Abstract
The genus Turbinaria stands out as a prominent and widely distributed member of the brown algae. This species is characterized by distinct morphology, ecological importance, and biochemical properties. In the present study, taxonomical, molecular identification along with antioxidant and anti-diabetic activity of the different extracts (acetone, aqueous & methanol) of T.decurrrens was determined. The preliminary phytochemical components were investigated qualitatively and quantitatively by using standard methods and confirmed the presence of variety of phytoactive components such as coumarins, glycosides, phenols tannins, terpenoids and saponins. The collected seaweed sample was identified by molecular approaches using QIAGEN DNA isolation kit and the BLAST program at the NCBI website that showed se quences similarities with existing seaweed rbcL gene sequences up to 99.21%. "The antioxidant activity of three crude extracts was evaluated by various antioxidant assays viz DPPH and ABTS." The findings revealed potent scavenging activity of ABTS, with a minimum inhibitory concentration (IC50) of 1.06mg/ml for the methanolic extract while the DPPH assay indicated an IC50 value 1.56mg/ml. The investigation into the antidiabetic properties of seaweed extract revealed that the methanolic extract of T.decurrens exhibited the most significant inhibitory activity against α-amylase and α-glucosidase enzymes with IC50 value of 1.8 mg/ml and 1.40mg/ml respectively. The presence of phytochemical metabolites contributes to both antioxidant activity and anti-diabetic activities. These results indicate that the extract of T.decurrens serve as a natural source of antioxidants and anti-diabetics compounds possessing the potential to address a wide array of serious health conditions.
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Menaa, F., Wijesinghe, P. A. U. I., Thiripuranathar, G., Uzair, B., Iqbal, H., Khan, B. A., & Menaa, B. (2020). Ecological and industrial implications of dynamic seaweed-associated microbiota interactions. Marine drugs, 18(12), 641.
Lomartire, S., & Gonçalves, A. M. (2022). An overview of potential seaweed-derived bioactive compounds for pharmaceutical applications. Marine Drugs, 20(2), 141.
(Lobban, C. S., & Harrison, P. J. (1994). Seaweed ecology and physiology. Cambridge University Press.)
El-Beltagi HS, Mohamed AA, Mohamed HI, Ramadan KMA, Barqawi AA, Mansour AT. Phytochemical and Potential Properties of Seaweeds and Their Recent Applications: A Review. Mar Drugs. 2022 May 24;20(6):342.
El Zokm, G.M.; Ismail, M.M. and El-Said, G.F. (2021). Halogen content relative to the chemical and biochemical composition of fifteen marine macro and micro algae: nutritional value, energy supply, antioxidant potency, and health risk assessment. Environmental Science and Pollution Research, 28(12): 14893–14908.
Din NAS, Mohd Alayudin 'S, Sofian-Seng NS, Rahman HA, Mohd Razali NS, Lim SJ, Wan Mustapha WA. Brown Algae as Functional Food Source of Fucoxanthin: A Review. Foods. 2022 Jul 27;11(15):2235.
Zubia, M., Stiger-Pouvreau, V., Mattio, L., Payri, C. E., & Stewart, H. L. (2020). A comprehensive review of the brown macroalgal genus Turbinaria JV Lamouroux (Fucales, Sargassaceae). Journal of Applied Phycology, 32, 2743-2760.
Rushdi MI, Abdel-Rahman IA, Saber H, Attia EZ, Abdelraheem WM, Madkour HA, Abdelmohsen UR. The genus Turbinaria: chemical and pharmacological diversity. Natural Product Research. 2021 Nov 18;35(22):4560-78.
(American Diabetes Association Professional Practice Committee, and American Diabetes Association Professional Practice Committee:. ‘2. Classification and diagnosis of diabetes: Standards of Medical Care in Diabetes—2022.’ Diabetes care 45.Supplement_1 (2022): S17-S38.)
(Baskaran, P., & Anuradha, C. V. (2011). Oxidative stress and diabetes mellitus: Role of antioxidants in its management. Free Radical Research, 45(7), 739-749. doi: 10.3109/10715762.2011.589643)
Papachristoforou, Eleftheria, et al. ‘Association of glycemic indices (hyperglycemia, glucose variability, and hypoglycemia) with oxidative stress and diabetic complications.’ Journal of diabetes research 2020.1 (2020): 7489795.
(Williams, G. M., Iatropoulos, M. J., & Whysner, J. (1999). ‘Safety Assessment of Butylated Hydroxyanisole and Butylated Hydroxytoluene as Antioxidants in Foods.’ Food and Chemical Toxicology, 37(9–10), 1027-1038 and Gharavi, N., El-Kadi, A. O., & Moore, M. M. (2007). ‘Tert-Butylhydroquinone Modulates Gene Expression and Induces Cytotoxicity in HepG2 Cells.’ Toxicology in Vitro, 21(5), 663-670.)
(Kumar, Yogesh, Ayon Tarafdar, and Prarabdh C. Badgujar. ‘Seaweed as a source of natural antioxidants: Therapeutic activity and food applications.’ Journal of Food Quality 2021.1 (2021): 5753391).
Sami, F. J., N. H. Soekamto, and J. Latip. ‘Total phenolic, antioxidant activity and toxicity effect of Turbinaria decurrens extracts from South Sulawesi.’ Journal of Physics: Conference Series. Vol. 1341. No. 3. IOP Publishing, 2019.
(Lordan S, Smyth TJ, Soler-Vila A, Stanton C, Ross RP.The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chem. 2013 Dec 1; 141(3):2170-6).
(Cheong, S. H., & Kim, J. H. (2015). Inhibition of α-amylase and α-glucosidase by seaweed extracts: Implications for the management of postprandial hyperglycemia. Journal of Food Science, 80(5), H1089-H1095. doi: 10.1111/1750-3841.12818)
(Ryu, J. W., Lee, M. S., Yim, M. J., Lee, D. S., Kim, Y. M., & Eom, S. H. (2023). α-amylase and α-glucosidase inhibition effects of Korean edible brown, green, and red seaweed extracts. Fisheries and Aquatic Sciences, 26(3), 181-187).
(Van de Laar, Floris A., et al. ‘α-Glucosidase inhibitors for patients with type 2 diabetes: results from a Cochrane systematic review and meta-analysis.’ Diabetes care 28.1 (2005): 154-163.)
(Zhong, L., Yang, J., Syed, J. N., Zhang, Y., Tian, Y., & Fu, X. (2025). Alpha-Glucosidase Inhibitors in Aging and Aging-Related Diseases: Clinical Applications and Relevant Mechanisms. Aging and disease.)
(Benzie, I. F., & Wachtel-Galor, S. (Eds.). (2011). Herbal medicine: biomolecular and clinical aspects).
Arif M., Li Y., El-Dalatony M.M., Zhang C., Li X., Salama E.-S. A complete characterization of microalgal biomass through FTIR/TGA/CHNS analysis: An approach for biofuel generation and nutrients removalmRenew. Energy, 163 (2021), pp. 1973-1982.
Nei M. and Kumar S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35:1547-1549.
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410.
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680.
Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39(4), 783–791. https://doi.org/10.2307/2408678
Sofowora, A. (1993). Screening Plants for Bioactive Agents. In: Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Spectrum Books Ltd, Sunshine House, Ibadan, Nigeria, pp. 134-156.
Trease, G.E. and Evans, W.C. (2002). Pharmacognosy. 15th Ed. Saunders Publishers, London. pp. 42-44, 221-229, 246-249, 304-306, 331-332, 391-393.
Vianna, Spectrophotometric determination of coumarins incorporated into nanoemulsions containg Pterocaulon balansae extract, Lat. Am. J. Pharm., № 30, с. 1487.
Solich P, Sedliakova V, Karlicek R. Spectrophotometric determination of cardiac glycosides by flow-injection analysis. Anal Chim Acta. 1992; 269(2): 199-203.
Singleton VL, Rossi JA. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture,16: 144-158.
Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem 2007; 105(3): 950-958.
Ghorai N., Chakraborty S., Gucchait S., Saha, S.K. and Biswas, S. 2012. Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent. Nature Protocol Exchange doi:10.1038/protex.2012.055.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26: 1231-1237.
Von Gadow A, Joubert E, Hansmann CF. 1997. Comparison of antioxidant activity of aspalathin with that of other plant phenols of Rooibosed tea (Aspalathon linearis), α-tocopherol, BHT and BHA. Journal of Agricultural and Food Chemistry, 45: 632-638.
McCue P.P and Shetty, K. ‘Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro,’ Asia Pacific Journal of Clinical Nutrition, vol. 13, no. 1, pp. 101–106, 2004.
Watanabe J, Kawabata J, Kurihara H, Niki R. Isolation and identification of alpha-glucosidase inhibitors from tochu-cha (Eucommia ulmoides). Biosci Biotechnol Biochem. 1997 Jan;61(1):177-8. doi: 10.1271/bbb.61.177. PMID: 9028049.
(Rao, P. S., Periyasamy, C., Kumar, K. S., & Rao, A. S. (2024). A Role of Algae in an Aquatic Ecosystem. In Algal Biotechnology (pp. 3-15). CRC Press.)
Ismail, G.A.; Gheda, S.F.; Abo-Shady, A.M. and Abdel-Karim, O.H. (2020). In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes. Food Science and Technology, 40(3): 681–691. https://doi.org/10.1590 /fst.15619
Ponnan, A., Ramu, K., Marudhamuthu, M., Marimuthu, R., Siva, K., & Kadarkarai, M. (2017). Antibacterial, antioxidant and anticancer properties of Turbinaria conoides (J. Agardh) Kuetz. Clinical Phytoscience, 3, 1-10.
(Afrin, F., Ahsan, T., Mondal, M. N., Rasul, M. G., Afrin, M., Silva, A. A., ... & Shah, A. K. M. A. (2023). Evaluation of antioxidant and antibacterial activities of some selected seaweeds from Saint Martin's Island of Bangladesh. Food Chemistry Advances, 3, 100393.
Dhanraj, Vijayraja, Manivasagam, Tamilarasan., & Karuppaiah, Jeyaprakash (2017). Myricetin isolated from Turbinaria ornata ameliorates rotenone induced parkinsonism in Drosophila melanogaster. International Journal of Pharmacy and Pharmaceutical Sciences, 9(10), 39-44.
Gupta S, Abu-Ghannam N. Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods. Innov Food Sci Emerg Technol 2011; 12: 600-609.
Ananthi, S., Raghavendran, H. R. B., Sunil, A. G., Gayathri, V., Ramakrishnan, G., & Vasanthi, H. R. (2010). In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Turbinaria ornata (Marine Brown Alga). Food and chemical toxicology, 48(1), 187-192.
(Hasani-Ranjbar S, Larijani B, Abdollah M. A systematic review of Iranian medicinalplants useful in diabetes mellitus. Arch Med Sci. 2008; 4:285–92. )
Azad, S. B., Ansari, P., Azam, S., Hossain, S. M., Shahid, M. I. B., Hasan, M., & Hannan, J. M. A. (2017). Anti-hyperglycaemic activity of Moringa oleifera is partly mediated by carbohydrase inhibition and glucose-fibre binding. Bioscience reports, 37(3), BSR20170059.
Arguelles, E. D., & Sapin, A. B. (2020). In vitro antioxidant, alpha-glucosidase inhibition and antibacterial properties of Turbinaria decurrens Bory (Sargassaceae, Ochrophyta). Asia-Pacific Journal of Science and Technology, 25(3).
Gunathilaka, T.; Rangee Keertihirathna, L. and Peiris, D. (2021). Advanced Pharmacological Uses of Marine Algae as an Anti-Diabetic Therapy. In Pharmacognosy - Medicinal Plants. IntechOpen. https://doi.org/10.5772/intechopen.96807.
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