Anti- Proliferative Activity Of Hylocereus Undatus Against Human Skin Melanoma (Skmel Cell Lines)
Keywords:
Hylocereus undatus, antioxidant property, anticancer property, cytotoxic activityAbstract
Hylocereus undatus (Dragon fruit) is a tropical fruit of cactus species (Cactaceae) which has red peel with green fins. It contains rich antioxidants (vitamins and minerals that helps to prevent cancer, cardiovascular, diabetics, gastrointestinal, respiratory, and urinary disease), and low calorie dietary fibres. Every year, millions of people die from cancer, and most anticancer medications have a low specificity of action and significant toxicity, resultant in severe side effects. In the present research, the antioxidant and anticancer property of ethanol fruit pulp extract of Hylocereus undatus was studied and the result revealed that the ethanol fruit pulp extract of H. undatus showed 35.33% of inhibition at the 100 mcg/ ml concentration and the standard ascorbic acid showed 96.61% of inhibition at the 100 mcg/ ml concentration. The result represented thatethanol fruit pulp extract of H. undatushave moderate antioxidant activity when compared to the standard ascorbic acid.The L929 cell lines (murine fibroblast) were treated with ethanol fruit pulp extract of H. undatus. The percentage of cell viability of normal L929 cell lines and SKMEL cell lines (human skin melanoma) were treated with different concentrations ofethanol fruit pulp extract of H. undatus (6.25µg/ml, 12.5µg/ml, 50 µg/ml, 100 µg/ml) and their cytotoxic activity against Normal and Human skin melanoma was analysed. The results showed that higher the concentration of ethanol fruit pulp extract of H. undatus lower the viability of cell lines followed by higher the cytotoxic activity on cell lines. Dose dependant reduction in cell viability was observed in SK-MEL cells administered with different concentrations of the ethanol fruit pulp extract of H. undatus. The IC50 value of ethanol fruit pulp extract of H. undatus in SKMEL cell line was 74.32 µg/mL of the sample. The present research reported that the ethanol fruit pulp extract of H. undatus have a good anticancer property against the skin melanoma cell line thus, the fruit of H. undatus can be used as a natural remedy for cancer without any side effects..
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Elmarzugi, N. A. (2016). Phytochemical properties and health benefits of Hylocereus undatus. Nanomed Nanotechnol, 1, 1–10.
[2] ● Guimarães, D. A. B., De Castro, D. S. B., Oliveira, F. L. D., Nogueira, E. M., Silva, M. A. M. D., & Teodoro, A. J. (2017). Pitaya extracts induce growth inhibition and proapoptotic effects on human cell lines of breast cancer via downregulation of estrogen receptor gene expression. Oxidative Medicine and Cellular Longevity, 2017(1), 7865073.
[3] ● Hossain, F. M., Numan, S. M., & Akhtar, S. (2021). Cultivation, nutritional value, and health benefits of dragon fruit (Hylocereus spp.): A review. International Journal of Horticultural Science and Technology 8, 239–249.
[4] ● Joseph, M.M., Aravind, S.R., Varghese, S., Mini, S. and Sreelekha, T.T., 2012. Evaluation of antioxidant, antitumor and immunomodulatory properties of polysaccharide isolated from fruit rind of Punicagranatum. Molecular medicine reports, 5(2), pp.489-496.
[5] ● Kevin P. Anthony and Mahmoud A. Saleh,Free Radical Scavenging and Antioxidant Activities of Silymarin Components. Antioxidants (Basel). 2013 Dec; 2(4): 398–407.
[6] ● Le Bellec F., Vaillant F., Imbert E. Pitahaya (Hylocereus spp.): A new fruit crop, a market with a future. Fruits. 2006; 61: 237–250. doi: 10.1051/fruits:2006021.
[7] ● Padmavathy, K., Kanakarajan, S., Karthika, S., Selvaraj, R., & Kamalanathan, A. (2021). Phytochemical profiling and anticancer activity of dragon fruit Hylocereus undatus extracts against human hepatocellular carcinoma cancer (HepG-2) cells. International Journal of Pharmaceutical Sciences and Research, 12, 2770–2778.
[8] ● Paśko, P., Galanty, A., Zagrodzki, P., Ku, Y. G., Luksirikul, P., Weisz, M., & Gorinstein, S. (2021). Bioactivity and cytotoxicity of different species of pitaya fruits–A comparative study with advanced chemometric analysis. Food Bioscience, 40, 100888.
[9] ● Singh, S., & Kumar, S. (2023). A review on nutritional, medicinal and bio-active compound of dragon fruit Hylocereus polyrhizus (FAC Weber) britton & rose. International Journal of Biochemistry Research & Review, 32, 57–67.
[10] ● Tang W., Li W., Yang Y., Lin X., Wang L., Li C., Yang R. Phenolic compounds profile and antioxidant capacity of pitahaya fruit peel from two red-skinned species (Hylocereus polyrhizus and Hylocereus undatus) Foods. 2021;10:1183. doi: 10.3390/foods10061183.
[11] ● Wu, L. C., Hsu, H. W., Chen, Y. C., Chiu, C. C., Lin, Y. I., & Ho, J. A. (2006). Antioxidant and antiproliferative activities of red pitaya. Food Chemistry, 95, 319–327.
[12] ● Wu, S. J., & Ng, L. T. (2008). Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia) Linn. LWT - Food Science and Technology, 41(2), 323–330.
[13] ● Wybraniec S., Mizrahi Y. Fruit flesh betacyanin pigments in Hylocereus cacti. J. Agric. Food Chem. 2002; 50: 6086–6089. doi: 10.1021/jf020145k.
[14] ● Yien Ong, Y., Siang Tan, W., Rosfarizan, M., Chan, E. S., & Ti Tey, B. (2012). Isolation and identification of lactic acid bacteria from fermented red dragon fruit juices. Journal of Food Science, 77, M560–M564
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