In-vitro antioxidant potential of plectranthus amboinicus (Lour) against phenyl hydrazine induced hemolysis
Keywords:
Plectranthus amboinicus (Lour.), Phenyl hydrazine, Na+/K+ ATPase, flavonoidsAbstract
The intracellular free radical-generating probe phenyl hydrazine (PHZ) is one of the most studied substances that causes oxidative damage in erythrocytes. Plectranthus amboinicus (Lour.) is a significant medicinal plant that is utilised in traditional Indian medical systems to treat a wide range of illnesses. Determining the PHZ-induced oxidative stress-mediated changes in goat red blood cells' (RBCs') membrane-bound enzymes, such as Na+/K+ ATPase, and how Plectranthus amboinicus (lour.) leaf juice can improve them is the prime aspect of this project work. The protective effect of FLJPA against PHZ-induced oxidative stress-mediated damages in goat RBCs in vitro was clearly demonstrated by the dose-dependent increase in the liberation of inorganic phosphate, i.e., 22.65, 30.44, 38.71, 48.11 and 57.41 µg of Pi liberated/min/mg of protein by various FLJPA treated groups at concentrations of 20, 40, 60, 80 and 100 µg/ml, respectively. This effect may have been attributed to the antioxidant principles of the FLJPA, such as tannins and flavonoids.
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[1] Jen-Yin Goh, Richard J. Weaver. Development and use of in vitro alternatives to animal testing by the pharmaceutical industry 1980–2013.Toxicol. Res, 2015;4: 1297–1307.
[2] Jose V Castell, In Vitro Methods in Pharmaceutical Research ,1., Academic Press
[3] ,1997,28-30
[4] De Oliveira S, Saldanha C, An overview about erythrocyte mem-brane.Clin Hemorheol Microcirc, 44 (2010) 63.
[5] Nicotera P, Moore M, Mirabelli F, Bellomo G, Orrenius S, Inhibition of hepatocyte plasma membrane Ca2+ - ATP ase activity by menadione metabolism and its restoration by thiols. FEBS Lett, 181 (1985) 149.
[6] Schrier SL, Mohandas N, Globin-chain specificity of oxidation- induced changes in red blood cell membrane properties. Blood, 79 ((1992) 1586.
[7] Widad NM, Al-Naama L, Meaad L, Trace element in patients with beta thalassemia major. Haem, 6 (2003) 376.
[8] Goldberg B, Stern A, The generation of O2-by the interaction of the hemolytic agent, phenylhydrazine, with human hemoglobin. J Biol Chem, 250 (1975) 2401.
[9] Goldberg B, Stern A, Peisach J, The mechanism of superoxide anion generation by the interaction of phenylhydrazine with hemoglobin. J Biol Chem, 251(1976) 3045.
[10] Haber F, Weiss J, The catalytic decomposition of hydrogen peroxide by iron salts. (Proc. R. Soc. London, Ser. A) ,147 (1934) 332.
[11] 10 Wijk RV, Wouter W, Solinge V, The energy-less red blood cell is lost: erythrocyte enzyme abnormalities of glycolysis. Blood, 106 (2015) 4034.
[12] Chauhan VP, Tsiouris JA, Chauhan A, Sheikh AM, Brown WT, Vaughan M. Increased oxidative stress and decreased activities of Ca(2+)/Mg(2+)-ATPase and Na(+)/K(+)-ATPase in the red blood cells of the hibernating black bear. Life Sci , 71(2002 ) 153.
[13] Banerjee M, Ray AK, The role of thyroid hormone on phenylhydrazine hydrochloride mediated inhibitory effects on blood acetylcholinesterase: an in vivo and in vitro study. J Biochem Mol Toxicol, 16 (2002) 162.
[14] Zavodnik IB, Lapshina EA , Rekawiecka K, Zavodnik LB, Bartosz G, Bryszewska M. Membrane effects of nitrite-induced oxidation of human red blood cells. Biochimica et Biophysica Acta, 1421 (1999) 306.
[15] WHO Technical Report Series 916, Diet, Nutrition and The Prevention of Chronic Diseases.2002.
[16] Tulp M and Bohlin L. Unconventional natural sources for future drug discovery. Drug Discov. Today,2004; 9: 450–458.
[17] Sato N . Roles of the acidic lipids sulfo-quinovosyldiacylglycerol and phosphatidyl- glycerol in photosynthesis: their specificity and evolution. J. PlantRes, 2004;11: 495–505.
[18] Willet WC. Diet and health: What should we eat? Science,1994;264:32-37. 18.Holland, B, Unwin, I.D. & Buss, D.H. Vegetables, herbs and spices.Royal Society of Chemistry, 1991;4(1): 163-164.
[19] Kelly LS, Dobson EL. Evidence concerning the origin of liver macrophages. Br J Exp Pathol. 1971 Feb;52(1):88–99.
[20] De Oliveira S, Saldanha C, An overview about erythrocyte mem-brane. Clin Hemorheol Microcirc, 44 (2010) 63.
[21] Opden Kamp JAF, Lipid asymmetry in membranes. Annu Rev Biochem, 48 (1979) 47.
[22] Asey JR, Reithmeier RAF, Analysis of the oligomeric state of band 3, the anion transport protein of the human erythrocyte membrane, by size exclusion high performance liquid chromatography, Oligomeric stability and origin of heterogeneity. J Biol Chem, 266 (1991) 15726.
[23] Bennett V, Baines AJ, Spectrin and ankyrin-based pathways: metazoan inventions for integrating cells into tissues. Physiol Rev, 81 (2001) 1353.
[24] Gonzales R, Auclair C, Voisin E, Gautero H, Dhermy D, Boivin P, Superoxide dismutase, catalase, and glutathione peroxidase in red blood cells from patients with malignant diseases. Cancer Res, 44 (1984) 4137.
[25] Nagababu E, Chrest FJ, Rifkind JM, Hydrogen-peroxide-induced heme degradation in red blood cells: the protective roles of catalase and glutathione peroxidase. Biochim. Biophys Acta, 1620 (2003) 211-276
[26] Bandyopadhyay U, Das D, Banerjee RK, Reactive oxygen species: Oxidative damage and pathogenesis. Current science, 77 ( 1999) 10.
[27] Ranvers , Über Pyrodin. Arb. A.d. erst. Med. Klin. Zu Berl. ii, 471(1891).
[28] Falconer E,Treatment of polycythemia: the reticulocyte response to venesection, phenylhydrazin and radiation. Ann Intern Med, 7 (1933) 172.
[29] Spivak JL, Polycythemia vera: myths, mechanisms, and management. Blood ,100 (2002) 4272.
[30] Giffin H, Allen E, The control and complete remission of polycythemia vera following the prolonged administration of phenyl hydrazine hydrochloride. Am J Med Sci, 185 (1933)1.
[31] Giffin H, Allen E, Experiments with phenyl hydrazine. I. Studies on the blood. Ann Intern Med, 1 (1928) 655.
[32] Giffin H, Conner H, The untoward effects of treatment by phenyl hydrazine hydrochloride. J Am Med Assoc, 92 (1929) 1505.
[33] Hara H, Ogawa M, Erythropoietic precursors in mice with phenyl hydrazine-induced anemia. Am J Hematol, 1 (1975) 453.
[34] Berger J, Screening of toxic-haemolytic anaemia in laboratory rats: a model of phenyl hydrazine induced haemolysis. Haematologia, 18 (1985)193.77
[35] Stern A, Drug-induced oxidative denaturation in red blood cells. Semin Hematol, 26 (1989) 301.
[36] Rifkind, Heinz body anemia: an ultrastructural study. II. Red cell sequestration and destruction. Blood, 26 (1965) 433.
[37] Rifkind RA, Danon D, Heinz Body anemia- An ultrastructural study. I. Heinz Body formation. Blood, 25 (1965) 885.
[38] McMillan DC, Jensen CB, Jollow DJ, Role of lipid peroxidation in dapsone-induced hemolityc anemia. J Pharmacol Exp Ther, 287 (1998) 868.
[39] Goldberg B, Stern A, The mechanism of oxidative hemolysis produced by phenyl hydrazine, Mol. Pharmacol, 13 (1977) 832.
[40] RoshanDhruv Patel. Antioxidant potential of leaves of Plectranthus amboinicus Lour.Der Pharmacia Lettre, 2010;2(4):240-245.
[41] Praveena Bhatt, Gilbert Stanely. Chemical composition and nutraceutical potential of Indian borage stem extract.Journal of Chemistry,2013;7(2):7-13.
[42] Gurgel AP, da Silva JG, GrangeiroAR .In vivo study of the anti-inflammatory and antitumor activities of leaves of Plectranthusamboinicus (Lour.). J Ethnopharmacol ,2009 ;125:361–363.
[43] K K Hullatti, Prasenjit Bhattacharjee. Pharmacognostical Evaluation of Different Parts of Coleus amboinicuslour. Lamiaceae . International Journal of Pharmacy and Pharmaceutical Sciences, 2011; 3(24):24-26.
[44] Suja S,Indumathi T.Protective role of Plectranthus amboinicus in aluminium induced neurotoxicity. international journal of Medicine and Pharmaceutical Sciences,2013;3:41-
[45] Pradeep S Negi, Praveen Bhatt. Antioxidant and anti-bacterial activities in the leaf extracts of Indian borage (Plectranthus amboinicus). Food and Nutrition Sciences,2012;3:146-152.
[46] Malini T.Antifertility effectbeta-sitosterol from Plectranthus amboinicus Lour. in male albino rats.Journal of Ethnopharmacol,1991;35(2):149-153.
[47] Chiu, Y.J. Analgesic and anti-inflammatory activities of the aqueous extracts from Plectarnthus amboinicus Lour, both In -vitro and In –vivo.2012;10(2): 25-36.
[48] Singh G, Singh OP, Prasad YR, Catalan C. Studies on essential oils: chemical and insecticidal investigations on leaf oil of Coleus amboinicus Lour, Flavour Fragr J, 2002; 17(6): 440–442.
[49] Satish Rao BS, Shanbhoge R, Upadhya D, Jagetia GC, Adiga SK, Kumar P, Guruprasad K &Gayathri P. Antioxidant, anticlastogenic and radio protective effect of Coleus aromaticus on Chinese hamster fibroblast cells (V79) exposed to gamma radiation, Mutagenesis, 2006; 21(4): 237–242.
[50] Pritima RA &Pandian RS. Antimicrobial activity of Coleus aromaticus (benth) against microbes of reproductive tract infections among women, Afr J Infect Dis, 2007; 1(1): 18–24.
[51] PonnusamyRevathi and Thangaraj Parimelazhagan. Anti-diabetic analysis and insulin expression study of a wild leafy vegetable Acalyphaalnifolia Klein ex wild. On streptozotocin-induced diabetic rats.Bangladesh J Pharmacol, 2015; 10: 461-466.
[52] Botelho MA, Nogueira NA, Bastos GM. Antimicrobial activity of the essential oil from Lippiasidoides, against oral pathogens. Braz J Med Biol Res,2007; 40(3):349–356.
[53] Subhaschandrappa M, Harsha R, Dinesha R, Thammannagowda SS. Antibacterial activity of coleus aromaticus leaves. Int J Pharm Pharm Sci.2010; 2(3):63–66.
[54] Wadikar DD &Premavalli KS, Karpurvalli beverage. A shelf stable ready-to-drink appetizer and process for preparation there of Indian patent, Application No. 1271;2011: 122-125.
[55] RoshanP,Naveen M, Anita S.Plectranthusamboinicus (Lour.) Spreng:Anoverview.The Pharma Research,2010;4:1-15.
[56] Gurgel AP, da Silva JG, Grangeiro AR . In vivo study of the anti-inflammatory and antitumor activities of leaves of Plectranthusamboinicus (Lour.). J Ethnopharmacol ,2009 ;125:361–363.
[57] SavinaAsiimwe, Anna-Karin Borg- Karlsson, Muhammad Azeem. Chemical composition and Toxicological evaluation of the aqueous leaf extracts of Plectranthus amboinicus Lour. Spreng, International Journal of Pharmaceutical Science Invention, 2014;3 (2):19-27.
[58] Sridhar Prasad Y P , Padmaja Hari , Shajina M , Mirshad P V,. Hematinic and antioxidant potential of aqueous extract of Sesamum indicum seeds against phenylhydrazine-induced hemolytic anemia in albino rats. National Journal of Physiology, Pharmacy and Pharmacology.2018;8(8)1092-1096.
[59] Hye Won Lee, Hyojun Kim, Jin Ah Ryuk, Ki-Jung Kil, Byoung Seob Ko. Hemopoietic effect of extracts from constituent herbal medicines of Samul-tang on phenylhydrazine- induced hemolytic anemia in rats. Int J Clin Exp Pathol ,2014;7(9):6179-6185.
[60] Pressley, T.A., R.S. Haber, J.N. Loeb, I.S. Edelman, and F. Ismail-Beigi (1986) J. Gen. Physiol. 87:591-606
[61] Pradip K. Sarkar . A Quick Assay for Na+-K+-ATPase Specific Activity. Zeitschrift fur Naturforschung C ,2002;2(3) 562-564.
[62] Sudeshna Paul, Shamreen Naaz, Arnab Kumar Ghosh, Sanatan Mishra, Aindrila Chattopadhyay, Debasish Bandyopadhyay .Melatonin chelates iron and binds directly with phenylhydrazine to provide protection against phenylhydrazine induced oxidative damage in red blood cells along with its antioxidant mechanisms: an in vitro study. Melatonin Res 2018; 1 (1): 1-20
[63] Saravana Babu ,Chidambaram s babu , Vijayan Ranju ,periyathambi thalaivani,sathiya sekar.Venthamarai chooranam, a polyherbal Siddha medicine, alleviates hypertension via AT(1)R and eNOS signaling pathway in 2K1C hypertensive rats.Research Gate.2014;239(6):15- 33
[64] .Integrated Taxonomic information system .Taxonomic serial number:504444.(htts://www.itis.gov)
[65] Rout OP, Rout KK, Acharya R, Mishra SK.Preliminarypharmacognostical and phytochemical evaluation of Coleus aromaticusBenth leaf. Int J Pharm World Res,2010;1(4):348–355.
[66] Gupta S, Lakshmi AJ, Manjunath MN, Jamuna P. Analysis of nutrient and antinutrient
[67] content of underutilized green leafy vegetables. Food SciTechnol, 2005; 38:339–345.
[68] Vallikannan B. Carotenoid composition and retinol equivalent in plants of nutritional and medicinal importance: efficacy of b-carotene from Chenopodium album in retinol
[69] deficient rats. Food Chem, 2010;119:1584–1590.
[70] Wadikar DD &Premavalli KS. Beverage from Coleus aromatics reduce leptin levels and improve appetite rating in human volunteers. Nutrition, 2014; 30(6): 702–705.
[71] Prajapati ND, Purohit SS, Sharma AK & Kumar T. A handbook of medicinal plants section-II: medicinal plants A to Z. 2010; Agribios, India
[72] Senthil K .Use of Pashanabheda Coleus aromaticus in ashmari (Urinary calculi). Natn. Med J 1967;9:475–477.
[73] Usmanghani K.,Mustafa SB, Mehmood Z, Akhter N, Kauser A, Hussain I, Rashid A, Akram M, Tahir IM, Munir N, Riaz M, Niazi SG, Ali A, Ashraf MM, Naz U, Ahmed H, Shah SMA.Review-Medicinal plants and management of Diabetes Mellitus: Pak J Pharm Sci.2016 ;29(5): 1885-1891
[74] Stephanie Richards. Calculating final, or working, concentrations for journal‐style scientific papers. Dept. of Biology, Bates College .
[75] John M. Walker ,The Protein Protocols Handbook, 2 , Humana press 2002, 15-20 .
[76] Arduini A, Storto S, Belfiglio M, Scurti R, Mancinelli G, Federici G, Mechanism of spectrin degradation induced by phenylhydrazine in intact human erythrocytes. Biochim Biophys Acta, 979 ((1989) 1.
[77] Paul S, Ghosh D, Ghosh AK, Bhowmick D, Bandyopadhyay D, Chattopadhyay A (2016) Aqueous bark extract of Terminalia arjuna protects against phenylhydrazine induced oxidative
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