Polystyrene biodegradation by Pseudomonas sp. strain PVGSPCDU isolated from Superworm gut
DOI:
https://doi.org/10.63682/jns.v14i32S.9751Keywords:
polystyrene, plastic, degradation, superworm, Pseudomonas spAbstract
Massive amounts of plastic garbage are a serious social problem. There have been several initiatives to address the issue of plastic trash, including techniques for the natural decomposition of plastic. Since polystyrene (PS) is currently one of the plastics most often utilized in numerous industries, its degradation is a serious worldwide concern. In this study, we identified a Pseudomonas sp. Strain PVGSPCDU bacterium that breaks down PS from the superworms' guts. The breakdown of PS by Pseudomonas sp. has hardly been investigated up to this point. Using electronic microscopy, we investigated PS degradation and recorded atomic distribution and contact angle changes with water droplets on the PS surface, which indicate a chemical transition from hydrophobicity to hydrophilicity. Using Fourier transform-infrared spectroscopy (FT-IR), we investigated chemical structural changes during PS degradation by Pseudomonas sp. to look for the formation of C=O bonds and shifts towards hydrophilicity. According to our research, Pseudomonas, which is found in the superworms' guts, contributes to the breakdown of plastics after consumption. Therefore, the results of this study are important because they show a possible remedy for PS degradation in addition to revealing a new role for Pseudomonas in superworms' guts
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1. Alvarez‐Ortega, C., & Harwood, C. S. (2007). Responses of Pseudomonas aeruginosa to low oxygen indicate that growth in the cystic fibrosis lung is by aerobic respiration. Molecular microbiology, 65(1), 153-165.
2. Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., ... & Beckham, G. T. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences, 115(19), E4350-E4357.
3. Bandyopadhyay, A., & Basak, G. C. (2007). Studies on photocatalytic degradation of polystyrene. Materials Science and Technology, 23(3), 307-314.
4. Bode, H. B., Zeeck, A., Plückhahn, K., & Jendrossek, D. (2000). Physiological and chemical investigations into microbial degradation of synthetic poly (cis-1, 4-isoprene). Applied and Environmental Microbiology, 66(9), 3680-3685.
5. Brandon, A. M., Gao, S. H., Tian, R., Ning, D., Yang, S. S., Zhou, J., ... & Criddle, C. S. (2018). Biodegradation of polyethylene and plastic mixtures in mealworms (larvae of Tenebrio molitor) and effects on the gut microbiome. Environmental science & technology, 52(11), 6526-6533.
6. Brandon, A. M., Garcia, A. M., Khlystov, N. A., Wu, W. M., & Criddle, C. S. (2021). Enhanced bioavailability and microbial biodegradation of polystyrene in an enrichment derived from the gut microbiome of Tenebrio molitor (mealworm larvae). Environmental science & technology, 55(3), 2027-2036.
7. Chaudhary, A. K., & Vijayakumar, R. P. (2020). Studies on biological degradation of polystyrene by pure fungal cultures. Environment, Development and Sustainability, 22(5), 4495-4508.
8. Hardalo, C., & Edberg, S. C. (1997). Pseudomonas aeruginosa: assessment of risk from drinking water. Critical reviews in microbiology, 23(1), 47-75.
9. Ho, B.T., Roberts, T.K. & Lucas, S. (2018) An overview on bio degradation of polystyrene and modified polystyrene: the microbial approach. Critical Reviews in Biotechnology, 38, 308– 320.
10. Kim, H. R., Lee, H. M., Yu, H. C., Jeon, E., Lee, S., Li, J., & Kim, D. H. (2020). Biodegradation of polystyrene by Pseudomonas sp. isolated from the gut of superworms (larvae of Zophobas atratus). Environmental science & technology, 54(11), 6987-6996.
11. Kim, H. R., Lee, H. M., Yu, H. C., Jeon, E., Lee, S., Li, J., & Kim, D. H. (2020). Biodegradation of polystyrene by Pseudomonas sp. isolated from the gut of superworms (larvae of Zophobas atratus). Environmental science & technology, 54(11), 6987-6996.
12. Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of molecular evolution, 16, 111-120.
13. Krieg N. R., Holt J. G. Bergey’s manual of systematic bacteriology 1: The Williams & Wilkins Co.; Baltimore.
14. Li, J., Kim, H. R., Lee, H. M., Yu, H. C., Jeon, E., Lee, S., & Kim, D. H. (2020). Rapid biodegradation of polyphenylene sulfide plastic beads by Pseudomonas sp. Science of the Total Environment, 720, 137616.
15. Lou, Y., Ekaterina, P., Yang, S. S., Lu, B., Liu, B., Ren, N., ... & Xing, D. (2020). Biodegradation of polyethylene and polystyrene by greater wax moth larvae (Galleria mellonella L.) and the effect of co-diet supplementation on the core gut microbiome. Environmental science & technology, 54(5), 2821-2831.
16. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.
17. Matjašič, T., Simčič, T., Medvešček, N., Bajt, O., Dreo, T., & Mori, N. (2021). Critical evaluation of biodegradation studies on synthetic plastics through a systematic literature review. Science of the Total Environment, 752, 141959.
18. Mihreteab, M., Stubblefield, B. A., & Gilbert, E. S. (2019). Microbial bioconversion of thermally depolymerized polypropylene by Yarrowia lipolytica for fatty acid production. Applied Microbiology and Biotechnology, 103, 7729-7740.
19. Mooney, A., Ward, P. G., & O’Connor, K. E. (2006). Microbial degradation of styrene: biochemistry, molecular genetics, and perspectives for biotechnological applications. Applied microbiology and biotechnology, 72, 1-10.
20. Palm, G. J., Reisky, L., Böttcher, D., Müller, H., Michels, E. A., Walczak, M. C., ... & Weber, G. (2019). Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate. Nature communications, 10(1), 1717.
21. Peng, B. Y., Chen, Z., Chen, J., Yu, H., Zhou, X., Criddle, C. S., ... & Zhang, Y. (2020). Biodegradation of polyvinyl chloride (PVC) in Tenebrio molitor (Coleoptera: Tenebrionidae) larvae. Environment International, 145, 106106.
22. Ru, J., Huo, Y., & Yang, Y. (2020). Microbial degradation and valorization of plastic wastes. Frontiers in Microbiology, 11, 442.
23. Santo, M., Weitsman, R., & Sivan, A. (2013). The role of the copper-binding enzyme–laccase–in the biodegradation of polyethylene by the actinomycete Rhodococcus ruber. International Biodeterioration & Biodegradation, 84, 204-210.
24. Shimao, M. (2001). Biodegradation of plastics. Current opinion in biotechnology, 12(3), 242-247.
25. Singh, B., & Sharma, N. (2008). Mechanistic implications of plastic degradation. Polymer degradation and stability, 93(3), 561-584.
26. Sneath, P. H. A. (1989). Analysis and interpretation of sequence data for bacterial systematics: the view of a numerical taxonomist. Systematic and Applied Microbiology, 12(1), 15-31.
27. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution, 30(12), 2725-2729.
28. Urbanek, A. K., Rybak, J., Wróbel, M., Leluk, K., & Mirończuk, A. M. (2020). A comprehensive assessment of microbiome diversity in Tenebrio molitor fed with polystyrene waste. Environmental Pollution, 262, 114281.
29. Weaver, D. K., & McFarlane, J. E. (1990). The effect of larval density on growth and development of Tenebrio molitor. Journal of Insect Physiology, 36(7), 531-536.
30. Wierckx, N., Prieto, M. A., Pomposiello, P., de Lorenzo, V., O'Connor, K., & Blank, L. M. (2015). Plastic waste as a novel substrate for industrial biotechnology. Microbial biotechnology, 8(6), 900.
31. Woo, S., Song, I., & Cha, H. J. (2020). Fast and facile biodegradation of polystyrene by the gut microbial flora of Plesiophthalmus davidis larvae. Applied and Environmental Microbiology, 86(18), e01361-20.
32. Wu, M., Guina, T., Brittnacher, M., Nguyen, H., Eng, J., & Miller, S. I. (2005). The Pseudomonas aeruginosa proteome during anaerobic growth. Journal of bacteriology, 187(23), 8185-8190.
33. Yang, Y., Wang, J., & Xia, M. (2020). Biodegradation and mineralization of polystyrene by plastic-eating superworms Zophobas atratus. Science of the total environment, 708, 135233.
34. Yang, Y., Yang, J., Wu, W. M., Zhao, J., Song, Y., Gao, L., ... & Jiang, L. (2015a). Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 1. Chemical and physical characterization and isotopic tests. Environmental science & technology, 49(20), 12080-12086.
35. Yang, Y., Yang, J., Wu, W. M., Zhao, J., Song, Y., Gao, L., ... & Jiang, L. (2015b). Biodegradation and mineralization of polystyrene by plastic-eating mealworms: part 2. Role of gut microorganisms. Environmental science & technology, 49(20), 12087-12093.
36. Yanto, D. H. Y., Krishanti, N. P. R. A., Ardiati, F. C., Anita, S. H., Nugraha, I. K., Sari, F. P., ... & Watanabe, T. (2019, August). Biodegradation of styrofoam waste by ligninolytic fungi and bacteria. In IOP Conference Series: Earth and Environmental Science (Vol. 308, No. 1, p. 012001). IOP Publishing.
37. Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199
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