Development and Evaluation of Timolol Maleate loaded Bilosomes.

Authors

  • Gangotri Yadav
  • Hrishikesh More
  • Ashish Jain
  • Shubham Waghmare
  • Gaurav Pandhare

DOI:

https://doi.org/10.63682/jns.v14i24S.9750

Keywords:

ocular delivery, Timolol maleate, bilosomes, in-situ gel.

Abstract

Objective : This study aimed to enhance anti-glaucoma activity of Timolol maleate by incorporating Timolol maleate into bilosomal pH-responsive in-situ gel for the treatment of Intra-ocular pressure caused by glaucoma.

Material and Method : The thin-film hydration technique was used to create bilosomes with different proportions of cholesterol, bile salts, and edge activators. Since it demonstrated the best drug entrapment efficiency, the thin-film hydration approach was chosen for additional development. At varying concentrations, the optimized bilosomal batch was incorporated to pH-responsive in-situ gels.


Results and Discussion : 0.1% Carbopol(934) gel demonstrated the highest drug content and was used for further analysis. The bilosomes were characterized for particle size (150–400 nm), polydispersity index, and entrapment efficiency (72%–95%). Transmission electron microscopy confirmed spherical morphology. The optimized bilosomal pH-responsive in-situ gel formulation exhibited favorable pH, viscosity, spreadability, and sustained in vitro drug release. Antimicrobial efficacy evaluated via the cup plate diffusion method revealed a larger zone of inhibition for the bilosomal in-situ gel, indicating enhanced therapeutic potential and antimicrobial effectiveness. These findings suggest its potential as an effective formulation for the treatment of Intra-ocular pressure caused by glaucoma .

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Published

2025-05-05

How to Cite

1.
Yadav G, More H, Jain A, Waghmare S, Pandhare G. Development and Evaluation of Timolol Maleate loaded Bilosomes. . J Neonatal Surg [Internet]. 2025 May 5 [cited 2026 Feb. 9];14(24S):1059-68. Available from: https://jneonatalsurg.com/index.php/jns/article/view/9750

Issue

Vol. 14 No. 24S (2025): Journal of Neonatal Surgery

Section

Original Article

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