Design , Development and Characterization of Asiaticoside loaded nanofibers.

Authors

  • Gangotri Yadav
  • Pratidnya Patil
  • Ashish Jain
  • Pratiksha Pukale
  • Shantanu Dusane

DOI:

https://doi.org/10.63682/jns.v14i14S.9749

Keywords:

Asiaticoside, centrifugal spinning, nanofibers, PVA, wound healing, modified release, entrapment efficiency

Abstract

Asiaticoside, a triterpenoid derived from Centella asiatica, has significant antibacterial, collagen-stimulating, and wound-healing effects, its low skin penetration and poor solubility restrict its clinical use. This study used centrifugal spinning to create and assess Asiaticoside-loaded nanofibers as a modified-release wound dressing. Using a cotton-candy-style spinneret (2000–2400 rpm), polyvinyl alcohol (8–12% w/w) was mixed with secondary polymers (sodium alginate or chitosan) and propylene glycol to create nanofibers. TLC, UV, FTIR, and DSC were used to screen nine formulations (F1–F9) for morphology, thickness and weight homogeneity, entrapment efficiency, and physicochemical compatibility. The improved formulation (NF5) showed high drug entrapment (≈89%), sustained release (>85% over 24 h), and smooth, bead-free fibers with diameters of 217–558 nm. Stability tests validated the preservation of physicochemical characteristics over 90 days, and antimicrobial testing against Staphylococcus aureus showed higher effectiveness compared to similar medication gel. These findings suggest that centrifugal-spun Asiaticoside nanofibers are a viable approach for enhanced wound healing applications and provide a feasible, solvent-effective platform for localized, controlled drug administration, improving therapeutic efficacy and antibacterial activity

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References

1. Sayali S, Pandav V, Jaiswal R, Kalam S. Transdermal Drug Delivery System. Int J Pharm Sci. 2024;02(08).

2. Saghazadeh S, Rinoldi C, Schot M, et al. Drug delivery systems and materials for wound healing applications. Adv Drug Deliv Rev. 2018;127:138-166.

3. Wildy M, Lu P. Electrospun Nanofibers: Shaping the Future of Controlled and Responsive Drug Delivery. Materials. 2023;16(22):7062.

4. Jiang Z, Zheng Z, Yu S, et al. Nanofiber Scaffolds as Drug Delivery Systems Promoting Wound Healing. Pharmaceutics. 2023;15(7):1829.

5. Abdulhussain A, Adebisi A, Conway BR, Asare-Addo K. Electrospun nanofibers: Exploring process parameters, polymer selection, and applications in pharmaceuticals. J Drug Deliv Sci Technol. 2023;90:105156.

6. Zhang X, Lu Y. Centrifugal Spinning: An Alternative Approach to Fabricate Nanofibers at High Speed and Low Cost. Polym Rev. 2014;54(4):677-701.

7. Silva A, Kuranage SW, Baji A. Centrifugal Force Spinning for Producing Polymer Fibers and Polymer Yarns. 2021.

8. Palani N, Vijayakumar P, Monisha P, Ayyadurai S, Rajadesingu S. Electrospun nanofibers synthesized from polymers incorporated with bioactive compounds for wound healing. J Nanobiotechnol. 2024;22(1).

9. Xie F, Gao C, Avérous L. Alginate-based materials: Enhancing properties through multiphase formulation design and processing innovation. Mater Sci Eng R. 2024;159:100799.

10. Liu L, Ding Z, Yang Y, Zhang Z, Lu Q, Kaplan DL. Asiaticoside-laden silk nanofiber hydrogels for scarless skin regeneration. Biomater Sci. 2021;9(15):5227-5236.

11. Li K, Zhu Z, Zhai Y, Chen S. Advances in electrospun nanofiber-based strategies for diabetic wound healing. Pharmaceutics. 2023;15(9):2285.

12. Zhu L, Liu X, Du L, Jin Y. Preparation of asiaticoside-loaded coaxially electrospinning nanofibers and their effect on deep partial-thickness burn injury. Biomed Pharmacother. 2016;83:33-40.

13. Liu XY, Zhu LF, Du LN, Jin YG. Preparation of asiaticoside-loaded coaxial and composite nanofibers and their sustained-release effects. J Int Pharm Res. 2016;43(2):330-335.

14. Razbin M, Sohrabi M, Boueini AT, Daryakenari AA, Abbasi M. Intelligent design and fabrication of nanofibrous bilayer air filters. Results Eng. 2025;28:107982.

15. Dragar Č, Roškar R, Kocbek P. Incorporated drug affects the properties of hydrophilic nanofibers. Nanomaterials. 2024;14(11):949.

16. Nourani M, Jafari SM. Electrospun fibers/nanofibers loaded with phase change materials: Characterization, applications and challenges. Polym Test. 2025;150:108879.

17. Khan SA, Khan SB, Latif U, Khan A, Asiri. Fourier Transform Infrared Spectroscopy: Fundamentals and Application in Functional Groups and Nanomaterials Characterization. 2019.

18. Sharma R, Yadav S, Yadav V, et al. Recent advances in lipid-based long-acting injectable depot formulations. Adv Drug Deliv Rev. 2023;199:114901.

19. Heunis TDJ, Smith C, Dicks LMT. Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus skin infections in mice. Antimicrob Agents Chemother. 2013;57(8):3928-3935.

20. International Conference on Harmonisation (ICH). Stability testing of new drug substances and products Q1A(R2). 2003.

21. Moldovan OL, Brihan DN, Todoran N, Rusu A. Thin layer chromatographic compatibility study in preformulation of transdermal systems. Acta Marisiensis Ser Med. 2020;66(1):23-29.

22. Chang W, Skandan G, Hahn H, Danforth SC, Kear B. Chemical Vapor Synthesis of Nanostructured Ceramics. MRS Proc. 1994;351.

23. Suwantong O, Ruktanonchai U, Supaphol P. Electrospun cellulose acetate fiber mats containing asiaticoside and release characteristics. Polymer. 2008;49(19):4239-4247.

24. Laidmäe I, Meos A, Kjærvik IA, et al. Electrospun amphiphilic nanofibers as templates for in situ preparation of chloramphenicol-loaded liposomes. Pharmaceutics. 2021;13(11):1742.

25. Ko E, Park KS, Kim S, Kim T, Kim H. Morphological study of cellulosic hydrogel nanofiber for biomedical application. Cellulose. 2019;26(17):9107-9118.

26. Weng J, Tong HHY, Chow SF. In vitro release study of polymeric drug nanoparticles: Method development and validation. Pharmaceutics. 2020;12(8).

27. Heunis TDJ, Smith C, Dicks LMT. Evaluation of a nisin-eluting nanofiber scaffold to treat Staphylococcus aureus-induced skin infections in mice. Antimicrob Agents Chemother. 2013;57(8):3928-3935.

28. Balu S, Jeyachandran S, Kimura M. Review on electrospinning of polymer nanofibers and biomedical applications. J Compos Sci. 2024;8(1):32.

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Published

2025-04-27

How to Cite

1.
Yadav G, Patil P, Jain A, Pukale P, Dusane S. Design , Development and Characterization of Asiaticoside loaded nanofibers. . J Neonatal Surg [Internet]. 2025 Apr. 27 [cited 2026 Mar. 10];14(14S):941-50. Available from: https://jneonatalsurg.com/index.php/jns/article/view/9749

Issue

Vol. 14 No. 14S (2025): Journal of Neonatal Surgery

Section

Original Article

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