Regenerative Medicine And Stem Cell Applications In Wound Healing And Scar Reduction
DOI:
https://doi.org/10.63682/jns.v14i26S.9524Keywords:
Regenerative Medicine, Stem Cell Therapy, Wound Healing, Scar Reduction, Cronbach's Alpha, Normality Test, Quantitative ResearchAbstract
Background:Stem cell therapies have emerged as promising treatments for wound healing and scar reduction due to their regenerative potential. This study aims to evaluate the perceived effectiveness, safety concerns, and overall impact of stem cell therapies in wound healing and scar reduction.
Objective:The primary objective of this study is to assess the perceptions of individuals who have undergone stem cell treatments or are involved in the healthcare sector regarding the effectiveness, safety, and outcomes of these therapies in the context of wound healing and scar reduction.
Methods:A quantitative, cross-sectional survey was administered to 250 participants, including patients who have received stem cell therapy for chronic wounds, burns, and scars, as well as healthcare professionals familiar with stem cell applications. The survey included Likert-scale and multiple-choice questions to assess participants' familiarity with stem cell therapies, their experiences with treatment, and their perceptions of its effectiveness and safety. Statistical analysis involved normality testing using the Shapiro-Wilk test, reliability analysis using Cronbach's Alpha, and descriptive and inferential statistics.
Results:Shapiro-Wilk normality tests revealed that the variable's Likelihood to Consider Therapy and Concern about Safety significantly deviated from normality (p-value < 0.05). The Cronbach’s Alpha for the reliability of the survey items was 0.652, indicating acceptable internal consistency but suggesting room for improvement in the reliability of the measurement tool. Visual analyses, such as bar and pie charts, were used to present the results of normality tests and reliability outcomes.
Conclusion:The findings suggest that stem cell therapies are perceived as effective in promoting wound healing and reducing scarring, though safety concerns, such as immune rejection and tumor formation, persist. The study highlights the need for non-parametric statistical methods and refined survey instruments to improve the reliability and accuracy of the findings. Future research should focus on larger, longitudinal studies to further evaluate the long-term efficacy and safety of stem cell therapies in clinical settings.
Downloads
References
Ahmed, F. A. M. (2025). Advances in Regenerative Medicine: Stem Cell Therapy and Tissue Engineering. The Open European Journal for Research in Medical and Basic Sciences (OEJRMBS), 39-54.
Akaberi, S. M., Sharma, K., Ahmadi-Ashtiani, H. R., & Hedayati, M. (2025). Polydeoxyribonucleotide in Skincare and Cosmetics: Mechanisms, Therapeutic Applications, and Advancements Beyond Wound Healing and Anti-aging. Journal of Skin and Stem Cell, 12(1).
Alves, J. C. (2025). Laser Therapy in Veterinary Regenerative Medicine. Laser Therapy in Veterinary Medicine: Photobiomodulation, 458-469.
Arcani, R., Velier, M., Sabatier, F., Simoncini, S., Abellan-Lopez, M., Granel, B., Benyamine, A., Gomes de Pinho, Q., Dani, V., & Gentile, P. (2025). Nanofat Use in Regenerative Medicine: A Systematic Literature Review and Consensus Recommendations from Expert Opinions. Facial Plastic Surgery & Aesthetic Medicine.
Bian, D., Wu, Y., Song, G., Azizi, R., & Zamani, A. (2022). The application of mesenchymal stromal cells (MSCs) and their derivative exosome in skin wound healing: a comprehensive review. Stem Cell Research & Therapy, 13(1), 24.
Bojanic, C., To, K., Hatoum, A., Shea, J., Seah, K. M., Khan, W., & Malata, C. M. (2021). Mesenchymal stem cell therapy in hypertrophic and keloid scars. Cell and tissue research, 383, 915-930.
Casado-Díaz, A., Quesada-Gómez, J. M., & Dorado, G. (2020). Extracellular vesicles derived from mesenchymal stem cells (MSC) in regenerative medicine: applications in skin wound healing. Frontiers in Bioengineering and Biotechnology, 8, 146.
Das, S., Thakur, A., Datta, A., Sahoo, A., Bandyopadhyay, S., & Sah, A. K. (2025). Advances in Regenerative Medicine for Orthopedic Injuries: A Comprehensive Review. Cureus, 17(2).
Fan, D., Zeng, M., Xia, Q., Wu, S., Ye, S., Rao, J., Lin, D., Zhang, H., Ma, H., & Han, Z. (2020). Efficacy and safety of umbilical cord mesenchymal stem cells in treatment of cesarean section skin scars: a randomized clinical trial. Stem Cell Research & Therapy, 11, 1-9.
Farabi, B., Roster, K., Hirani, R., Tepper, K., Atak, M. F., & Safai, B. (2024). The efficacy of stem cells in wound healing: a systematic review. International Journal of Molecular Sciences, 25(5), 3006.
Fukutome, A., Sakamoto, T., Asawa, Y., Riu, D., Kawakami, H., Hoshi, K., & Hikita, A. (2025). Establishment of a mouse organ culture model of fetal cleft lip for the evaluation of adipose-derived stem cell therapy. Regenerative Therapy, 28, 41-50.
Gentile, P., Sterodimas, A., Calabrese, C., & Garcovich, S. (2021). Systematic review: Advances of fat tissue engineering as bioactive scaffold, bioactive material, and source for adipose-derived mesenchymal stem cells in wound and scar treatment. Stem Cell Research & Therapy, 12(1), 318.
Guillamat-Prats, R. (2021). The role of MSC in wound healing, scarring and regeneration. Cells, 10(7), 1729.
Ho, J., Yue, D., Cheema, U., Hsia, H. C., & Dardik, A. (2023). Innovations in stem cell therapy for diabetic wound healing. Advances in Wound Care, 12(11), 626-643.
Hoseini, S. M., Azad, F. S., Zare, E., & Montazeri, F. (2025). Therapeutic potential of amniotic fluid and amniotic fluid-derived stem cells in regenerative medicine. Regenerative Biomedicine, 1(2), 85-105.
Jin, Y., Li, S., Yu, Q., Chen, T., & Liu, D. (2023). Application of stem cells in regeneration medicine. MedComm, 4(4), e291.
Jones, R. E., Foster, D. S., Hu, M. S., & Longaker, M. T. (2019). Wound healing and fibrosis: current stem cell therapies. Transfusion, 59(S1), 884-892.
Khalili, M. R., Ahmadloo, S., Mousavi, S. A., Joghataei, M. T., Milan, P. B., Gharahgheshlagh, S. N., Mohebi, S. L., Haramshahi, S. M. A., & Sarmadi, V. H. (2025). Navigating mesenchymal stem cells doses and delivery routes in heart disease trials: A comprehensive overview. Regenerative Therapy, 29, 117-127.
Khalilzad, M. A., Mohammadi, J., Amirsaadat, S., Najafi, S., Zare, S., Nilforoushzadeh, M. A., Khalilzad, M., Khaghani, A., Soltankouhi, M. R. F., & Hajimohammad, A. (2025). Elevating Dermatology Beyond Aesthetics: Perinatal-Derived Advancements for Rejuvenation, Alopecia Strategies, Scar Therapies, and Progressive Wound Healing. Stem cell reviews and reports, 1-21.
Kucharzewski, M., Rojczyk, E., Wilemska-Kucharzewska, K., Wilk, R., Hudecki, J., & Los, M. J. (2019). Novel trends in application of stem cells in skin wound healing. European journal of pharmacology, 843, 307-315.
Kumar, D., Pandey, S., Shiekmydeen, J., Kumar, M., Chopra, S., & Bhatia, A. (2025). Therapeutic Potential of Microneedle Assisted Drug Delivery for Wound Healing: Current State of the Art, Challenges, and Future Perspective. AAPS PharmSciTech, 26(1), 1-29.
Lopes, B., Sousa, P., Alvites, R., Branquinho, M., Sousa, A., Mendonça, C., Atayde, L. M., & Maurício, A. C. (2021). The application of mesenchymal stem cells on wound repair and regeneration. Applied Sciences, 11(7), 3000.
Ma, C., Li, Y., Liu, B., Deng, J., Gao, X., Zhang, H., Zhang, B., Zhou, Q., Peng, X., & Zhang, H. (2025). Exosomes derived from adipose mesenchymal stem cells promote corneal injury repair and inhibit the formation of scars by anti-apoptosis. Colloids and Surfaces B: Biointerfaces, 247, 114454.
Majewska, L., Dorosz, K., & Kijowski, J. (2025). Efficacy of Rose Stem Cell‐Derived Exosomes (RSCEs) in Skin Treatment: From Healing to Hyperpigmentation Management: Case Series and Review. Journal of Cosmetic Dermatology, 24(1), e16776.
Margiana, R., Markov, A., Zekiy, A. O., Hamza, M. U., Al-Dabbagh, K. A., Al-Zubaidi, S. H., Hameed, N. M., Ahmad, I., Sivaraman, R., & Kzar, H. H. (2022). Clinical application of mesenchymal stem cell in regenerative medicine: a narrative review. Stem Cell Research & Therapy, 13(1), 366.
Minhas, W. R., Saleemi, H., Raheem, M. F., Khalid, S. S., Aslam, H., & Ahmad, W. (2024). Crispr-Engineered Microbes For Enhanced Biodegradation Of Recalcitrant Pollutants A Genomic Approach To Environmental Remediation. Frontiers in Health Informatics, 13(3).
Monavarian, M., Kader, S., Moeinzadeh, S., & Jabbari, E. (2019). Regenerative scar-free skin wound healing. Tissue Engineering Part B: Reviews, 25(4), 294-311.
Nasiri, N., Haghdoost, F., Habibi, M., Fateh, M., Gholami, S., & Tabaie, S. M. (2024). Stem Cell Perspective for Regenerative Wound Healing: from Biology toward Future Clinical Directions: A Review. Cell Journal (Yakhteh), 26(10).
Nourian Dehkordi, A., Mirahmadi Babaheydari, F., Chehelgerdi, M., & Raeisi Dehkordi, S. (2019). Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies. Stem Cell Research & Therapy, 10, 1-20.
Shelke, G. S., Saleem, A., Shukla, D., & Arjumand, B. (2025). Advances In Bioactive Dental Composites. Metallurgical and Materials Engineering, 972-981.
Shimizu, Y., Ntege, E. H., & Sunami, H. (2022). Current regenerative medicine-based approaches for skin regeneration: A review of literature and a report on clinical applications in Japan. Regenerative Therapy, 21, 73-80.
Tashiro, S. (2025). Further treatment strategies to enhance regenerative rehabilitation for cell therapy in chronic spinal cord injury: a concise review of preclinical studies. Regenerative Medicine Reports, 2(2), 67-71.
Tottoli, E. M., Dorati, R., Genta, I., Chiesa, E., Pisani, S., & Conti, B. (2020). Skin wound healing process and new emerging technologies for skin wound care and regeneration. Pharmaceutics, 12(8), 735.
Wang, A. Y. L., Kao, H.-K., Liu, Y.-Y., & Loh, C. Y. Y. (2025). Engineered Extracellular Vesicles Derived from Pluripotent Stem Cells: A Cell-Free Approach to Regenerative Medicine. Burns & Trauma, tkaf013.
Wu, K. Y., Kearn, N., Truong, D., Choulakian, M. Y., & Tran, S. D. (2025). Advances in Regenerative Medicine, Cell Therapy, and 3D Bioprinting for Corneal, Oculoplastic, and Orbital Surgery. In. Springer.
Yang, R., Liu, F., Wang, J., Chen, X., Xie, J., & Xiong, K. (2019). Epidermal stem cells in wound healing and their clinical applications. Stem Cell Research & Therapy, 10, 1-14.
Zahedi, P., Ghaderi, G., Shekarriz, H., Rahimi, P., Panahian, A., Salmani, M., Movahed, N. Y., Parsaei, H., & Jafari, D. (2025). Designer Engineered Exosomes: a New Regenerative Medicine Platform for Skin Wound Healing. Regenerative Engineering and Translational Medicine, 1-18.
Zare, S., Jafarzadeh, A., Zare, S., & Shamloo, A. (2025). Exploring the dermatological applications of human mesenchymal stem cell secretome: a comprehensive review. Stem Cell Research & Therapy, 16(1), 177
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.