A Novel Insight into the Anti-Urolithiatic Effect of Manjistha and Triphala Syrup Formulation
Keywords:
Rubia cordifolia, Triphala, Anti-urolithiatic, Herbal syrup, Calcium oxalate, Litholytic activityAbstract
Urolithiasis is a common, painful renal disorder of calcium oxalate crystal precipitation. Some traditional medicines have been found to have anti-urolithiatic actions, including Manjistha (Rubia cordifolia) and Triphala. This study investigates the litholytic potential of a polyherbal syrup made from these plants. Hydroalcoholic extracts of R. cordifolia roots and Triphala were prepared by Soxhlet extraction. The herbal syrup was then subjected to anti-urolithiatic testing in vitro, through methodologies such as egg membrane assay and sedimentary crystal formation model. Cystone was taken as a reference standard. TLC was adopted for phytochemical profiling of the extracts. TLC helped identify the main phytoconstituents such as gallic and ellagic acids. Using the egg membrane assay method, the dissolution of calcium oxalate crystals was found to be 48% by the herbal syrup, while for Cystone this value was 54%. The sedimentary crystal model revealed a significant reduction in calcium oxalate crystals under treatment as compared with the control. The preparation showed promising effects of anti-urolithiatic activity in vitro, which could be due to synergistic phytoconstituent effects. These findings support its traditional usage and its potential for the natural treatment of urolithiasis. It is further suggested that investigating the activity in vivo, followed by clinical experiments, would be recommended for its confirmation.
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Vithursha S, Paheerathan V and Senevirathne AAI. In-vitro Evaluation of Anti Urolithiatic Activity of Cuminum Cyminum Seed Extract on Calcium Oxalate Stone. Journal of Natural & Ayurvedic Medicine. 2023;7(1):1-5.
Stamatelou, K., & Goldfarb, D. S. Epidemiology of Kidney Stones. Healthcare (Basel, Switzerland). 2023;11(3):424.
Glazer K, Brea I, J Leslie, et al. Ureterolithiasis, StatPearls Publishing, 2024.
Dawson, C. H., & Tomson, C.R. Kidney stone disease: pathophysiology, investigation and medical treatment, Clinical medicine (London, England), 2012, 12(5), 467–471.
Thakore P, Liang TH. Urolithiasis. [Updated 2023 Jun 5]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan.
Shafi, H., Moazzami, B., Pourghasem, M., & Kasaeian, A. An overview of treatment options for urinary stones. Caspian journal of internal medicine.2016; 7(1):1–6.
Prasad K, Sujatha D, Bharathi K. Herbal drugs in urolithiasis - a review. Pharmacognosy Res. 2007;1(1):175-9.
Sharvari Kakad, Nibha Bajpai, Manjistha (Rubia Cordifolia): A Herbal Treasure of India, International Journal for Multidisciplinary Research, 2024, 6(4).
Tarasiuk A, Mosińska, P., & Fichna, J, Triphala: current applications and new perspectives on the treatment of functional gastrointestinal disorders. Chinese medicine.2018;13:39.
Dr. BhanuPratap Singh, Dr. Om Prakash Dadhich, Dr. Deepa; A Review Study Of Medicinal Uses of Manjistha (Rubia Cordifolia).2017;5(8):1394-1401.
Bhagat, Madhulika, Indian Gooseberry (Emblica officinalis): Pharmacognosy Review, 2014, 2:471.
Sharma, Panshul & Verma, Kumar & Raj, Hans et al. A review on ethnobotany, phytochemistry and pharmacology on Terminalia belerica (Bibhitaki). Journal of Drug Delivery and Therapeutics, 2021, 11(1):173-181.
Meher, Sudhanshu & Bhuyan, G.C. & Das, Banamali & Panda, Purnendu & Ratha, Kshirod. (2018). Pharmacological Profile of Terminalia chebula Retz. and Willd. (Haritaki) in Ayurveda with Evidences. Research Journal of Pharmacology and Pharmacodynamics, 2018, 10(3).
Nyeem, Mohammad & Mannan, Md. Rubia cordifolia-phytochemical and Pharmacological evaluation of indigenous medicinal plant, International Journal of Physiology, Nutrition and Physical Education 2018; 3(1): 766-771.
M., devasena & S., lekshmi & mohanan, arun et al. pharmacology of triphala with special focus on their chemical constituents. International Journal of Applied Pharmaceutics, 2022, 14, 38-43.
Wen M, Chen Q, Chen W, Yang J, et al. A comprehensive review of Rubia cordifolia L.:Traditional uses, phytochemistry, pharmacological activities, and clinical applications. Frontiers in pharmacology, 2022, 13.
Gowda, D. & Muguli, Ganesh & Deshpande, et al. Phytochemical and pharmacological actions of triphala: Ayurvedic formulation - A review. International Journal of Pharmaceutical Sciences Review and Research, 2012, 15(2), 61-65.
Fotsing YS, Bankeu KJJ, El-Saber GB, et al. Extraction of Bioactive Compounds from Medicinal Plants and Herbs. In IntechOpen; 2021.
Reddy P, Gupta RK, Reddy S. Analgesic and anti-inflammatory activity of hydroalcoholic extract of Piper betle leaves in experimental animals. International journal of basic and clinical pharmacology.2016;5(3):979–85.
Gupta R, Gupta B. Phytochemical analysis of Manjishtha (Rubia cordifolia Linn.) and it’s therapeutic relevance. The Pharma Innovation Journal.2017;6(7):164-169.
Ahamed Shaheen Yasar S, Sridevi Kotekar, Nagapati Prabhakar Bhat, Roopa P. Nayak. Anxiolytic effects of stem of Rubia cordifolia ethanol extract on anxiety models in Wistar albino rats. MRIMS Journal of Health Sciences.2024;10:13-24.
Pal S.K, Shukla S.K, Verma S, Pratap S. Formulation and Evaluation of Madhuca Longifolia Extract Syrup For Lithiasis. Int. Journal of Pharmaceutical Sciences and Medicine (IJPSM).2023;8(7):1-20.
S. Raj, M.S. Gnana Soundara Rajan, S. Ramasamy. An in vitro Anti-urolithiasis Activity of a Herbal Formulation: Spinacia oleracea L. and Coriandrum sativum L. Clinical Complementary Medicine and Pharmacology. 2004;4(1):100-124.
Anu V, Akhila S, Kumar IA and Antony S: In-vitro antiurolithiatic activity of macerated aqueous extract of Terminalia chebula by using titrimetry method. Int J Pharmacognosy 2020; 7(6):144-47.
Vithursha S, Paheerathan Vand Senevirathne AAI. In-vitro Evaluation of Anti Urolithiatic Activity of Cuminum Cyminum Seed Extract on Calcium Oxalate Stone. Journal of Natural & Ayurvedic Medicines. 2023;7(1): 1-5.
Hennequin C, Lalanne V, Daudon M, Lacour B, Drueke T. A new approach to studying inhibitors of calcium oxalate crystal growth. Urological research.1993;21(2):101-108.
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