A Comprehensive Study On Formulation And Standardization Parameters For Polyherbal Intimate Wash
Keywords:
Formulation, Standardization Parameters, Polyherbal Intimate Wash, High- Performance Thin-Layer Chromatography, Medicinal HerbsAbstract
Objectives:
The study aimed to develop and standardize a polyherbal intimate wash as a natural cosmeceutical alternative. Objectives included qualitative and quantitative standardization of herbal extracts, formulation development, stability assessment, user acceptability evaluation, and comparison with commercial products. Additionally, the formulation's anti-inflammatory, antifungal, and antioxidant activities were assessed in vitro.
Methods:
Selected herbs were extracted using suitable techniques and standardized through phytochemical screening and marker compound estimation. A polyherbal intimate wash was formulated using these standardized extracts. The formulation was evaluated for physicochemical properties, stability, and user acceptability. In vitro assays were conducted to determine anti-inflammatory, antifungal, and antioxidant activities. Comparative analysis was performed against commercially available vaginal wash products to assess efficacy.
Results:
The in-house formulation (IHF) demonstrated the highest antimicrobial activity against Candida albicans with a 24 mm zone of inhibition, compared to FFVW (18 mm) and marketed VW (10 mm). Anti-inflammatory activity, evaluated via protein denaturation assay, was found to be 82.72 ± 31.52% for IHF, 85.45 ± 1.09% for FFVW, and 84.77 ± 2.11% for VW. Antioxidant activity, measured using the hydrogen peroxide scavenging assay, was 92.80 ± 0.24% for IHF and 92.75 ± 0.40% for FFVW, indicating strong free radical scavenging potential.
Conclusion:
The study successfully developed and standardized a stable and effective polyherbal intimate wash with significant antimicrobial, anti-inflammatory, and antioxidant properties. High user acceptability and comparable or superior performance to commercial products suggest strong potential for market positioning as a safe and effective herbal cosmeceutical product
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