Analytical Method Development And Validation For The Estimation Of Serratiopeptidase Using Spectrophotometry
Keywords:
Serratiopeptidase, Analytical Method Development,, UV-Visible spectrophotometry, Quality Control, HPLC, ELISA, Method ValidationAbstract
This study focuses on develop a simple, reliable, and cost effective analytical method for accurately measuring serratiopeptidase, a clinically significant anti-inflammatory and proteolytic enzyme, using UV-Visible spectrophotometry. Serratiopeptidase is a proteolytic enzyme.and has various therapeutic treatment mostly used as an anti-inflammatory and analgesic agent. And it makes accurate quantification in pharmaceutical formulation crucial for quality control and regulatory compliance. UV spectrophotometry is a affordable , simple, and mostly used for this purpose. Currently, analytical methods such as HPLC and ELISA are commonly employed for the estimation of serratiopeptidase, but these techniques are often costly, require advanced instrumentation, and involve complex sample preparation, which may not be feasible for routine quality control, particularly in small-scale pharmaceutical industries or academic research laboratories.This review summarizes the current strategies for the method development of serratiopeptidase estimation using UV spectrophotometry. Key parameters such as wavelength selection, solvent choice, sample preparation, method validation, and potential interferences are discussed to highlight the advantages and limitations of UV-based methods.
Downloads
Metrics
References
1.Bhosale AV, Pawar AY. “Development and validation of UV spectrophotometric method for estimation of serratiopeptidase.” Int J Pharm Sci Res, 2014.
Sandhya, V. K., Devi Gayathri, S., & Mathew, T. S. (2008). Quantitation of serrapeptase in formulations by UV method in the microplate format. Current Drug Delivery, 5(4).
M. N. Rahman et al., "Analytical method development and validation of serratiopeptidase", J Pharm Anal, 2020.
Singh, D., Saraf, S., Dixit, V. K., & Saraf, S. (2007). Spectrophotometric determination of serratiopeptidase and metronidazole in combined dosage forms. Biosciences Biotechnology Research Asia, 4(1), 305–306.
Yasotha, S., Manivannan, R., Sidheeque, O. K., Gowtham, S., Kathiravan, P. S., & Ramanan, B. (2023). Method development, validation, and forced degradation study of serratiopeptidase by UV spectrometric method. International Journal of Pharmaceutical and Applied Sciences, 12(1), 100–109.
Pandya, E. J., Kapupara, P., & Shah, K. V. (2014). Development and validation of simultaneous estimation of diclofenac potassium, paracetamol, and serratiopeptidaseby first order derivative UV spectroscopy method in pharmaceutical formulation. Journal of Chemical and Pharmaceutical Research, 6(5), 912–924.
Patel, P., & Patel, H. (2013). Spectrophotometric Determination of Serratiopeptidase and Metronidazole in Combined Dosage Forms. Asian Journal of Biochemical and Pharmaceutical Research, 3(2), 186–191.
Majima Y. et al., "Efficacy of Serrapeptase in Reducing Postoperative Swelling," Journal of International Medical Research, 2001.
Bhagat M. et al., "Therapeutic potential of serratiopeptidase: A review," International Journal of Pharmacy and Pharmaceutical Sciences, 2013.
Borse, A. S., & Sabale, S. P. (2014). UV Visible Spectrophotometric Method for the Simultaneous Estimation of Serratiopeptidase and Diclofenac Sodium in Their Bulk and Combined Dosage Form. International Journal of Pharmaceutical Sciences and Research, 5(6), 2504–2509.
Gupte, V., & Luthra, U. (2017). Analytical techniques for serratiopeptidase: A review. Journal of Pharmaceutical Analysis, 7(4), 203–207.
Limbasiya, A. B., & Kapupara, P. P. (2014). Development and validation of analytical method for simultaneous estimation of diclofenac potassium and serratiopeptidase in pharmaceutical formulation. Research Journal of Pharmaceutical Technology, 7(6), 655–659.
Pandya, E. J., & Shah, K. V. (2014). Development and validation of spectrophotometric methods for simultaneous estimation of diclofenac sodium and serratiopeptidase in pharmaceutical dosage form. World Journal of Pharmaceutical and Pharmaceutics, 3(12), 1279–1291.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
