Evaluation of primary cytokines and lactate dehydrogenase from nasopharyngeal secretion of Acute Otitis Media (AOM)

Authors

  • Prerna Singh
  • Prashanth Kumar Madoori
  • Pawan Toshniwal
  • Seema Toshniwal

Keywords:

Acute otitis Media, Nasopharyngeal secretions, Lactate dehydrogenase, Cytokines

Abstract

Background: Acute otitis media (AOM) is a common side effect of a viral upper respiratory tract infection (URI). The severity of nasopharyngeal cellular injury during URI, as evaluated by lactate dehydrogenase (LDH) concentrations in nasopharyngeal secretions (NPSs), was hypothesized to be associated with AOM complications.

Methodology: LDH concentrations were measured in NPS samples (n = 110) obtained during the initial URI followed by the development of AOM. Interleukin-6 (IL-6), and tumor necrosis factor- α (TNF-α)- concentrations were also measured. Lactate Dehydrogenase (LDH) and cytokines were measured by Immunoassay methods. The ELISA-based immunoassay Limit of Detection (LOD) for LDH is 0.1-1.0 mU/ml and the conventional Enzyme-Linked Immunosorbent assay (ELISA) LOD for IL-6 and TNF-α is from 0.5 to 5.0 pg/ml and 0–15 pg/ml, respectively.

Results: LDH concentrations independently predicted AOM complications. LDH concentrations were higher in URIs (P≤0.05). There was a positive correlation (P<0.001) between LDH concentrations and all cytokines.

Conclusion: LDH concentrations in NPS are related to an increased risk of AOM, implying that the severity of nasopharyngeal inflammatory injury during URI contributes to the development of AOM and that reducing inflammatory injury may lessen the risk of AOM.

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Published

2025-06-09

How to Cite

1.
Singh P, Kumar Madoori P, Toshniwal P, Toshniwal S. Evaluation of primary cytokines and lactate dehydrogenase from nasopharyngeal secretion of Acute Otitis Media (AOM). J Neonatal Surg [Internet]. 2025Jun.9 [cited 2025Jun.20];14(31S):531-5. Available from: https://jneonatalsurg.com/index.php/jns/article/view/7208

Issue

Vol. 14 No. 31S (2025): Journal of Neonatal Surgery

Section

Original Article

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