Biochemical, Histopathological, and Functional Evaluation of the Antiurolithiatic Efficacy of Tribulus terrestris in Ethylene Glycol-Induced Nephrolithiasis in Rodent Models

Authors

  • Swati S. Gaikwad
  • Rizwan A Bhaijamal
  • M. Sudha
  • Namrata Shailesh Khadake
  • Runashree Borah
  • Manoj Kashyap
  • Pooja Arora
  • Gourab Biswas

Keywords:

Tribulus terrestris, Gokshura, urolithiasis, nephroprotective activity, antiurolithiatic effect, ethylene glycol, renal stones, calcium oxalate crystals, medicinal plants

Abstract

The present study investigated the antiurolithiatic potential of methanolic extract of Tribulus terrestris (Gokshura) in ethylene glycol-induced urolithiasis in Sprague Dawley rats. Urolithiasis was experimentally induced by administering 0.75% ethylene glycol in drinking water for 28 days. The study design included curative and preventive groups treated with T. terrestris extract at 200 mg/kg and 400 mg/kg doses, along with a standard drug-treated group. Acute toxicity studies confirmed the extract's safety up to 2000 mg/kg as per OECD guideline 423. Biochemical estimations of urinary and serum parameters such as calcium, phosphorus, creatinine, uric acid, and blood urea nitrogen were performed. Histopathological evaluation and physical parameters like urinary volume and kidney weight were also assessed. Results indicated a significant reduction in lithogenic factors in both treatment models, with more pronounced effects in the preventive group. The extract significantly decreased calcium and phosphate excretion, normalized serum biochemical markers, improved urine output, and prevented kidney enlargement. Histological analysis supported the protective effect by showing reduced tubular damage and crystal deposition. Overall, the findings validate the traditional use of Tribulus terrestris in urolithiasis and suggest its potential as a safe and effective phytotherapeutic agent for preventing and managing renal stone formation.

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References

Abufaraj, M., Al Karmi, J., & Yang, L. (2022). Prevalence and trends of urolithiasis among adults. Curr Opin Urol, 32(4), 425-432. https://doi.org/10.1097/mou.0000000000000994

Assimos, D. G. (2021). Urolithiasis/Endourology. J Urol, 205(1), 298-300. https://doi.org/10.1097/ju.0000000000001446

Barros, A. P. A., Silva, I. S., Correa, L. C., & Biasoto, A. C. T. (2022). Effect of the cold pre-fermentative maceration and aging on lees times on the phenolic compound profile, antioxidant capacity and color of red sparkling wines. J Food Sci Technol, 59(8), 3245-3255. https://doi.org/10.1007/s13197-022-05531-z

Bervinova, A. V., Palikov, V. A., Mikhailov, E. S., Palikova, Y. A., Borozdina, N. A., Kazakov, V. A., Rudenko, P. A., Tukhovskaya, E. A., Dyachenko, I. A., Slashcheva, G. A., Goryacheva, N. A., Sadovnikova, E. S., Kravchenko, I. N., Kalabina, E. A., Shinelev, M. V., Wu, P., & Murashev, A. N. (2022). Efficacy of Ficus tikoua Bur. extract in ethylene glycol-induced urolithiasis model in SD rats. Front Pharmacol, 13, 974947. https://doi.org/10.3389/fphar.2022.974947

Fernández-Lázaro, D., Fernandez-Lazaro, C. I., Seco-Calvo, J., Garrosa, E., Adams, D. P., & Mielgo-Ayuso, J. (2022). Effects of Tribulus terrestris L. on Sport and Health Biomarkers in Physically Active Adult Males: A Systematic Review. Int J Environ Res Public Health, 19(15). https://doi.org/10.3390/ijerph19159533

Fernández-Lázaro, D., Mielgo-Ayuso, J., Del Valle Soto, M., Adams, D. P., González-Bernal, J. J., & Seco-Calvo, J. (2021). The Effects of 6 Weeks of Tribulus terrestris L. Supplementation on Body Composition, Hormonal Response, Perceived Exertion, and CrossFit(®) Performance: A Randomized, Single-Blind, Placebo-Controlled Study. Nutrients, 13(11). https://doi.org/10.3390/nu13113969

Ghanbari, A., Akhshi, N., Nedaei, S. E., Mollica, A., Aneva, I. Y., Qi, Y., Liao, P., Darakhshan, S., Farzaei, M. H., Xiao, J., & Echeverría, J. (2021). Tribulus terrestris and female reproductive system health: A comprehensive review. Phytomedicine, 84, 153462. https://doi.org/10.1016/j.phymed.2021.153462

Hinojosa-Gonzalez, D. E., & Eisner, B. H. (2023). Biomarkers in Urolithiasis. Urol Clin North Am, 50(1), 19-29. https://doi.org/10.1016/j.ucl.2022.09.004

Kachkoul, R., Touimi, G. B., El Mouhri, G., El Habbani, R., Mohim, M., & Lahrichi, A. (2023). Urolithiasis: History, epidemiology, aetiologic factors and management. Malays J Pathol, 45(3), 333-352.

Laddha, A. P., Murugesan, S., & Kulkarni, Y. A. (2022). In-vivo and in-silico toxicity studies of daidzein: an isoflavone from soy. Drug Chem Toxicol, 45(3), 1408-1416. https://doi.org/10.1080/01480545.2020.1833906

Martimbianco, A. L. C., Pacheco, R. L., Vilarino, F. L., Latorraca, C. O. C., Torloni, M. R., & Riera, R. (2020). Tribulus Terrestris for Female Sexual Dysfunction: A Systematic Review. Rev Bras Ginecol Obstet, 42(7), 427-435. https://doi.org/10.1055/s-0040-1712123 (Tribulus terrestris para disfunção sexual feminina: Uma Revisão Sistemática.)

Nerlekar, N., Patil, P., Khot, S., Kulkarni, A., Dandge, P., Berde, A., Kamane, S., Ghatage, P., & Dandge, P. (2024). Cold maceration extraction of wild fruit Terminalia bellirica (Gaertn.) Roxb.: exploring its bioactives for biomedical applications. Prep Biochem Biotechnol, 54(7), 982-1000. https://doi.org/10.1080/10826068.2024.2313632

Niyomchan, A., Chatgat, W., Chatawatee, B., Keereekoch, T., Issuriya, A., Jaisamut, P., Chusri, S., & Kunworarath, N. (2023). Safety Evaluation of the Polyherbal Formulation NawaTab: Acute and Subacute Oral Toxicity Studies in Rats. Evid Based Complement Alternat Med, 2023, 9413458. https://doi.org/10.1155/2023/9413458

Patel, V. B., & Acharya, N. (2020). Effect of Macrotyloma uniflorum in ethylene glycol induced urolithiasis in rats. Heliyon, 6(6), e04253. https://doi.org/10.1016/j.heliyon.2020.e04253

Quhal, F., & Seitz, C. (2021). Guideline of the guidelines: urolithiasis. Curr Opin Urol, 31(2), 125-129. https://doi.org/10.1097/mou.0000000000000855

Salehi-Pourmehr, H., Tayebi, S., DalirAkbari, N., Ghabousian, A., Tahmasbi, F., Rahmati, F., Naseri, A., Hajebrahimi, R., Mehdipour, R., Hemmati-Ghavshough, M., Mostafaei, A., & Hajebrahimi, S. (2023). Management of urolithiasis in pregnancy: A systematic review and meta-analysis. Scand J Surg, 112(2), 105-116. https://doi.org/10.1177/14574969221145774

Ștefănescu, R., Tero-Vescan, A., Negroiu, A., Aurică, E., & Vari, C. E. (2020). A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules, 10(5). https://doi.org/10.3390/biom10050752

Tzelves, L., Türk, C., & Skolarikos, A. (2021). European Association of Urology Urolithiasis Guidelines: Where Are We Going? Eur Urol Focus, 7(1), 34-38. https://doi.org/10.1016/j.euf.2020.09.011

Zhao, J., Tian, X. C., Zhang, J. Q., Li, T. T., Qiao, S., & Jiang, S. L. (2023). Tribulus terrestris L. induces cell apoptosis of breast cancer by regulating sphingolipid metabolism signaling pathways. Phytomedicine, 120, 155014. https://doi.org/10.1016/j.phymed.2023.155014

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Published

2025-05-27

How to Cite

1.
S. Gaikwad S, A Bhaijamal R, Sudha M, Khadake NS, Borah R, Kashyap M, Arora P, Biswas G. Biochemical, Histopathological, and Functional Evaluation of the Antiurolithiatic Efficacy of Tribulus terrestris in Ethylene Glycol-Induced Nephrolithiasis in Rodent Models. J Neonatal Surg [Internet]. 2025May27 [cited 2025Sep.21];14(28S):505-14. Available from: https://jneonatalsurg.com/index.php/jns/article/view/6640

Issue

Vol. 14 No. 28S (2025): Journal of Neonatal Surgery

Section

Original Article

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