Isolation and characterization of Dimethoate- resistant Nitrogen fixing Bacteria from Grapevine Soil

Authors

  • Ashwini V. Jadhav
  • Shraddha Mule
  • Pathade G. R

Keywords:

dimethoate, pesticide resistance, nitrogen fixation, cellulose degradation, pesticide tolerance, etc

Abstract

This study investigates the isolation and characterization of nitrogen fixing bacteria resistant to dimethoate, a commonly used organophosphate pesticide in vineyards. Soil samples from vineyards in Walwa, Sangli, Maharashtra were collected and tested for pesticide resistant bacteria. Six bacterial isolates PN1, PN2, PN3, PN4, PN5 and PN6 were exposed to dimethoate and their morphological, biochemical and enzymatic properties were analyzed. The isolates exhibit varying degrees of resistance to dimethoate with PN1 and PN3 showing the highest tolerance up to 1.2% and 1.0% respectively. Additionally, these isolates demonstrated the ability to fix nitrogen, solubilize phosphate and degrade cellulose, highlighting their potential for improving soil fertility and contributing to sustainable agricultural practices. Further studies are in progress to explore the practical applications of these isolates in bioremediation and crop growth promotion

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References

Baldani, J. I., Reis, V. M., Videira, S. S., Boddey, L. H., & Baldani, V. L. D. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil, 384(1-2), 413-431.

Masuda, Y., Mise, K., Xu, Z., Zhang, Z., Shiratori, Y., Senoo, K., & Itoh, H. (2024). Global soil metagenomics reveals distribution and predominance of Deltaproteobacteria in nitrogen-fixing microbiome. Microbiome, 12, 95.

Zerin, T. (2021). Isolation and characterization of nitrogen-fixing bacteria from soil sample in Dhaka, Bangladesh. ResearchGate.

Cappuccino, J. G., & Sherman, N. (2014). Microbiology: A Laboratory Manual. Pearson.

Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., & Williams, S. T. (1994). Bergey's Manual of Determinative Bacteriology. Williams & Wilkins.

Glick, B. R. (2012). Plant growth-promoting bacteria: mechanisms and applications. Scientifica, 2012.

Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. *Plant and Soil, 255(2), 571-586..

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Published

2025-05-19

How to Cite

1.
Jadhav AV, Mule S, G. R P. Isolation and characterization of Dimethoate- resistant Nitrogen fixing Bacteria from Grapevine Soil. J Neonatal Surg [Internet]. 2025May19 [cited 2025Sep.17];14(24S):871-5. Available from: https://jneonatalsurg.com/index.php/jns/article/view/6067