Phytochemical Investigation and HPTLC Screening of Tinospora Cordifolia leaf Extract
Keywords:
Gallic Acid, HPTLC analysis, Phytochemical screening, Quercetin, Tinospora cordifoliaAbstract
Background: Tinospora cordifolia, a vital medicinal plant in traditional Indian medicine, possesses immunomodulatory, antioxidant, anti-inflammatory, hepatoprotective, and antidiabetic properties. This study aimed to analyze the phytochemical composition and quantify key bioactive compounds in T. cordifolia leaf extract using High-Performance Thin-Layer Chromatography (HPTLC).
Methods: Fresh leaves of T. cordifolia were collected, authenticated, and subjected to Soxhlet extraction using 70% ethanol. Preliminary phytochemical screening identified carbohydrates, alkaloids, flavonoids, phenols, proteins, and amino acids. HPTLC was performed with a mobile phase of Toluene: Ethyl Acetate: Methanol: Formic Acid (5:6:2:1) and detected at 270 nm. Quantification of Rutin, Gallic Acid, and Quercetin was conducted using calibration curves, with validation as per ICH guidelines.
Results: The ethanolic extract had a 26% yield. HPTLC analysis demonstrated distinct bands for Gallic Acid and Quercetin with Rf values of 0.59 and 0.73, respectively, while Rutin (Rf 0.18) was not detected. Quantitative analysis revealed Gallic Acid content between 1.234% and 1.282%, and Quercetin content from 0.62% to 0.655%. The method showed high linearity (r² > 0.99), precision, specificity, and robustness.
Conclusion: T. cordifolia leaf extract is a valuable source of bioactive compounds, particularly Gallic Acid and Quercetin. The HPTLC method developed is simple, precise, and reliable, suitable for routine analysis of phytoconstituents in herbal formulations. These findings support the therapeutic potential of T. cordifolia and its use in developing standardized herbal medicines.
Downloads
Metrics
References
Dhama K, Sachan S, Khandia R, Munjal A, Iqbal HMN, Latheef SK, et al. Medicinal and Beneficial Health Applications of Tinospora cordifolia (Guduchi): A Miraculous Herb Countering Various Diseases/Disorders and its Immunomodulatory Effects. Recent Pat. Endocr. Metab. Immune Drug Discov. 2017;10:96–111.
Sam DS. Importance and effectiveness of herbal medicines. J. Pharmacogn. Phytochem. 2019;8:354–7.
Sharma P, Dwivedee BP, Bisht D, Dash AK, Kumar D. The chemical constituents and diverse pharmacological importance of Tinospora cordifolia. Heliyon 2019;5:e02437.
Saha S, Ghosh S. Tinospora cordifolia: One plant, many roles. Anc. Sci. Life 2012;31:151–9.
Ganaie, Agnihotri R, Sharma R. Free Radical Scavenging Activity of Tinospora cordifolia (Willd.) Miers. J. Pharmacogn. Phytochem. 2014;3:63–9.
Balkrishna A, Kumar MH, Gupta AK, Coloroma PN. COMPARATIVE ANALYSIS OF HPTLC, SECONDARY METABOLITES AND ANTIOXIDANT ACTIVITIES OF TINOSPORA CORDIFOLIA STEM POWDERS. . E 7.
Thorat N, Dodiya T, Prajapati D. HPTLC Method Development and Validation for Simultaneous Estimation of Berberine, Ellagic Acid and Ferulic Acid in Amrtadi Churna. J. Nat. Remedies 2022;649–57.
Girme A, Parmar V, Jagtap S, Saste G, Modi S, Hingorani L. Development and validation of UHPLC-ESI-MS/MS bioanalytical method, ADMET profiling, and pharmacokinetic study of bioactive phytoconstituents from Ayurvedic botanical Guduchi (Tinospora cordifolia). J. Pharm. Biomed. Anal. Open 2023;2:100018.
Rai P, Garain T, Gupta D. Phytochemical analysis of Tinospora cordifolia and Withania somnifera and their therapeutic activities with special reference to COVID-19. World J. Exp. Med. 2024;14:95512.
Shervani ZA, Mishra PK. Phytochemical Study of Tinospora Cordifolia Grown on Three Different Soil Conditions. Res. J. Life Sci. Bioinforma. Pharm. Chem. Sci. 05.
Patel U, Girme A, Patel K, Ghule C, Hingorani L, Gandhi T. A validated HPTLC method for quantification of cordifolioside A, 20-β-hydroxyecdysone and columbin with HPTLC–ESI–MS/MS characterization in stems of Tinospora cordifolia. J. Planar Chromatogr.--Mod. TLC 2021;34:217–28.
Kumar D, Verma N, Raj V. Quantitative Analysis of Gallic Acid and Quercetin by HPTLC and In Vitro Antioxidant Activity of Averrhoa carambola Linn. Orient. J. Chem. 2023;39:727–35.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
