Physicochemical Characterization and Preparation of Bacteriological Media Using Coconut Water and Milk

Authors

  • Shilpa S. Ruikar
  • Avadhut Thorat
  • Avadhut Thorat
  • G. R. Pathade

Keywords:

Coconut water, Coconut Milk, Bacteriological Media, Nutrient Media, Physicochemical analysis

Abstract

Coconut water and coconut milk have gained attention as promising alternatives for culture media preparation due to their nutrient-rich composition, offering a sustainable and cost-effective substitute for traditional microbial culture media ingredients. These natural substances support the growth of a variety of microorganisms, including bacteria, fungi, and yeast. Their ability to enhance microbial growth makes them valuable in microbiology and biotechnology. This study aimed to explore the physicochemical properties of coconut water and coconut milk, crucial for their application in culture media.The study also included the formulation of nutrient media using coconut water and coconut milk, which was subsequently evaluated for bacterial growth.  The analysis covered parameters such as pH, temperature, total dissolved solids, and nutrient content. Coconut water exhibited a pH range between 4.5 and 5.5, while coconut milk had a higher pH range of 6.5 to 7.5, both suitable for microbial growth. Their nutrient content, including sugars, protein content , and  fat content , also indicated strong potential for supporting microbial growth. The results of this study highlight coconut water and coconut milk as viable candidates for use in microbiological culture media. Their physicochemical properties not only facilitate microbial proliferation but also offer flexibility in media formulation. This research opens the door for further exploration of coconut-based culture media, offering a sustainable solution in microbiology with potential applications across different microorganisms

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Published

2025-05-16

How to Cite

1.
Ruikar SS, Thorat A, Thorat A, Pathade GR. Physicochemical Characterization and Preparation of Bacteriological Media Using Coconut Water and Milk. J Neonatal Surg [Internet]. 2025May16 [cited 2025Nov.25];14(24S):686-92. Available from: https://jneonatalsurg.com/index.php/jns/article/view/6028

Issue

Vol. 14 No. 24S (2025): Journal of Neonatal Surgery

Section

Original Article

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