The Formulation of Combination Nanochitosan Black Soldier Fly (Hermetica Illucens) Pupae and Demineralized Dentin Matrix (DDM) from Human Teeth Promotes Accelerated Bone Remodeling

Authors

  • Renie Kumala Dewi
  • Sri Oktawati
  • Asdar Gani
  • Eko Suhartono
  • Nurlinda Hamrun
  • Rasmidar Samad
  • Nurhayaty Natsir
  • Maharani Lallyza Apriasari

Keywords:

Nanochitosan, Demineralized Dentin Matrix (DDM), Bone Remodeling

Abstract

Background. Tooth extraction is a procedure to remove teeth from inside the socket which can cause damage to the tooth socket area. Black Soldier Fly (BSF) (Hermetia illucent) contains as much as 35% chitin found in shedding and cocoons. Chitin can be converted to chitosan. Nanochitosan is the smallest part of chitosan. Chitosan has high osteoconductivity properties that promote bone regeneration. Demineralized Dentin Matrix (DDM) came from human teeth that have been extracted is an organic material obtained from dentin that has osteogenic capabilities. Objective. To determine the potential of the combination of nanochitosan BSF pupae and DDM in forming RANKL, and OPG after tooth extraction. Metode. The left mandibular incisive tooth guinea pig is extracted, then the socket is filled with a Polyethylene glycol gel as a placebo in the control group (n=9), and the treatment group (n=9) is filled with a combination of nanochitosan BSF pupae and DDM from human teeth gel. The gel is inserted into the socket until it is full then suture with non-absorbable silk. The guinea pig in euthanasia saw the expression of RANKL, and OPG on the 7th, 14th, and 21st days. The RANKL, OPG, and Osteocalcin research results using Oneway ANOVA (p<0.05).
Result. There is a significant difference between the treatment and control group with the significance value in the expression RANKL, OPG (0.000) (p<0.05). Conclusion: Combination of nanochitosan BSF pupae and DDM from human teeth has an effect in increasing the expression of Osteoprotegerin (OPG) and decreasing RANKL on the -7th, -14th, and -21st days and might help increase bone remodeling.

Downloads

Download data is not yet available.

References

Kim YK, Ku JK. Extraction socket preservation. Journal of the Korean Association of Oral and Maxillofacial Surgeons. Korean Association of Oral and Maxillofacial Surgeons. 2020;46: 9-439. DOI: https://doi.org/10.5125/jkaoms.

Dewi RK, Oktawati S, Gani A, Suhartono E, Hamrun N, Qomariyah L. Potention Black Soldier Fly’s (Hermetia illucens) Live for Wound Healing and Bone Remodeling: A Systematic Review. AMJ. 2023;63(6): 9815-9833.

Sulistiawaty L, Puspita F. Isolasi dan Karakterisasi Kitin dan Kitosan dari Pupa Black Soldier Fly (BSF). Warta Akab. 2022;46(1): 56-63.

Sularsih. Penggunaan scaffold kitosan-aloe vera terhadap proliferasi sel fibroblas pada penyembuhan luka pasca pencabutan gigi cavia cobaya. Jurnal Material Kedokteran Gigi. 2018;7(2):24-32.

Bashir SM, Ahmed Rather G, Patrício A, Haq Z, Sheikh AA, Shah MZ ul H, Singh H, Khan AA, Imtiyaz S, Ahmad SB, Nabi S, Rakhshan R, Hassan S, Fonte P. Chitosan Nanoparticles: A Versatile Platform for Biomedical Applications. Materials. MDPI; 2022; 15(6521): 1-28. DOI: https://doi.org/10.3390/ma15196521.

Gao X, Qin W, Wang P, Wang L, Weir MD, Reynolds MA, Zhao L, Lin Z, Xu HKH. Nano-structured demineralized human dentin matrix to enhance bone and dental repair and regeneration. Applied Sciences (Switzerland). MDPI AG; 2019;9(1013): 1-14. DOI: 10.3390/app9051013.

Nuraeni N, Sulistijowati R. Aktivitas Antioksidan Dan Antibakteri Sedian Edible Kompleks Kitosan-Ekstrak Buah Mangrove Sonneratia alba. Jambura Fish Processing Journal. 2021 Jul 6;3(2):51–9. DOI: https://doi.org/10.37905/jfpj.v3i2.10649.

Kabir MdA, Murata M, Kusano K, Akazawa T, Shibata T. Autogenous Demineralized Dentin Graft for Third Molar Socket Regeneration-A Case Report. Dentistry. 2015;5(11): 1-4. DOI: 10.4172/2161-1122.1000343.

Sularsih, Soeprijanto. Perbandingan jumlah sel osteoblas pada penyembuhan luka antara penggunaan kitosan gel 1% dan 2%. Jurnal Material Kedokteran Gigi. 2012;1(2):145–52.

Hikmah N, Shita ADP. Peran Rankl pada Proses Resorpsi Tulang Alveolar Kondisi Diabetes. Stomatognatic Jurnal Kedokteran Gigi. 2013;10(3):105109.

Herniyati H, Narmada IB, Soetjipto S. The Role of Rankl and Opg in Alveolar Bone Remodeling and Improvement of Orthodontic Tooth Movement Post Coffee Brew Administration. Journal of International Dental and Medical Research. 2017;10(1):84-8.

Thomas SDC. Abnormal laboratory results: Bone turnover markers. Aust Prescr. 2012;35(5):156–8.

Adventa Y, Zubaidah N. The Role Of Hydroxyapatite Materials On Collagen Synthesis In Alveolar Bone Defects Healing. Conservative Dentistry Journal. 2021;11(1):24.

Zhang C, Liao Q, Ming JH, Hu GL, Chen Q, Liu SQ, Li YM. The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model. Acta Cir Bras. 2017 Jun 1;32(6):418–28. DOI: http://dx.doi.org/10.1590/s0102-865020170060000002.

Kazimierczak P, Koziol M, Przekora A. The Chitosan/Agarose/Nanoha Bone Scaffold-Induced M2 Macrophage Polarization and Its Effect On Osteogenic Differentiation In Vitro. Int J Mol Sci. 2021;22(3):1–14. DOI: https://doi.org/10.3390/ijms22031109.

Hartomo BT, Firdaus FG. Pemanfaatan Biomaterial Kitosan Dalam Bidang Bedah Mulut. B-Dent: Jurnal Kedokteran Gigi Universitas Baiturrahmah. 2015;6(1):63-70.

Dewi RK, Oktawati S, Gani A, Suhartono E, Hamrun N, Rohmanna NA, Kaswati NMN, Analita R. Synthesis, Characterization, and Insilico Nanochitosan of Pupa Black Soldier Fly (Hermetia Illucens) As Bone Graft Material for Bone Remodeling Post Tooth Extraction. Journal of Chemical Health Risks. 2023;13(4):2370-2377.

Nadia LMH, Suptijah P, Ibrahim B. Produksi Dan Karakterisasi Nano Kitosan Dari Cangkang Udang Windu Dengan Metode Gelasi Ionik. JPHPI. 2014;17(2):119-127.

Koga T, Minamizato T, Kawai Y, Miura KI, Takashi I, Nakatani Y, Sumita Y, Ashina I. Bone Regeneration Using Dentin Matrix Depends On The Degree Of Demineralization And Particle Size. PLoS One. 2016;11(1):1-12. DOI: 10.1371/journal.pone.0147235.

Ding M, Xue L, Zeng Y, Fang C, Cheng W, Li Y. The Progress in the Repair of Bone Defect with Demineralized Dentin Matrix. J Oral Health Dent Sci. 2020;4(102): 1-4.

Gao X, Qin W, Wang P, Wang L, Weir MD, Reynolds MA, Zhao L, Lin Z, XU HHK. Nano-Structured Demineralized Human Dentin Matrix To Enhance Bone And Dental Repair And Regeneration. Applied Sciences (Switzerland). 2019;9(1013):1-14. DOI: 10.3390/app9051013.

Nagy V, Penninger JM. The RANKL-RANK Story. Gerontology. 2015;61: 534–42. DOI: 10.1159/000371845.

Downloads

Published

2025-05-10

How to Cite

1.
Kumala Dewi R, Oktawati S, Gani A, Suhartono E, Hamrun N, Samad R, et al. The Formulation of Combination Nanochitosan Black Soldier Fly (Hermetica Illucens) Pupae and Demineralized Dentin Matrix (DDM) from Human Teeth Promotes Accelerated Bone Remodeling. J Neonatal Surg [Internet]. 2025 May 10 [cited 2026 Apr. 1];14(21S):1136-42. Available from: https://jneonatalsurg.com/index.php/jns/article/view/5556

Issue

Vol. 14 No. 21S (2025): Journal of Neonatal Surgery

Section

Original Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

You are free to:

  • Adapt — remix, transform, and build upon the material for any purpose, even commercially.

Terms:

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.