Influence Of Ultrasonic Waves on The Activity of Above and Nadp - Dependent Liver Isocytedehydrogenase in Rats and Ways of Their Correction

Authors

  • Gafurdjon Mukhamedov
  • Dilnoza Babakhanova
  • Parida Mirkhamidova
  • Rano Alimova
  • Gulnara Shakhmurova
  • Otabek Eshonkulov

Keywords:

hepatocyte, mitochondria, isocitrate dehydrogenase, extract, biosep, ultrasound wave

Abstract

Isocitrate dehydrogenase is a key enzyme in the tyrcarboxylic acid cycle and is involved in the oxidative decarboxylation of isocitrate (IC) to form alpha-ketoglutarate (α-ketoglutarate) and CO2. The goal was to investigate the effect of ultrasonic waves on the activity of lipid peroxidation, isocitrate dehydrogenase in rat liver mitochondria, as well as the effect of mulberry leaf extract and biosep oil extract. The experiment involved white lab rats weighing 180-200 grams each. The ultrasound device used was Mindrey DP-50 Vet. The work is based on the following methods: Schneider differential centrifugation, spectrophotometry, chromatography, pH-metry, photometry. The article presents data on the suppression of the activity of NAD and NADP-dependent isocitrate dehydrogenase (IDH) enzymes in liver mitochondria in rats irradiated with ultrasound for 5 minutes. Deep inhibition of enzymes was observed on the 1st and 3rd days after exposure to ultrasonic waves (ultrasound), which in turn led to disruption of membrane structures and changes in lipid peroxidation processes in rat liver mitochondria. In the groups of rats, in which the correction was carried out with mulberry extract and biosep oil extract, restoration of the activity of NAD and NADP-IDH enzymes in liver mitochondria was observed.

The obtained data show that the correction effects of mulberry extract were more effective than those of biosep oil extract

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References

Мирхамидова П., Бабаханова Д.Б., Умарова Г.Б., Кадырова Д.А. биологическая химия. – Ташкент: Изд-во Навруз. 2018. – 366 с

Лившиц В.М. Биохимические анализы в клинике / В.М Лившиц, В.И.Сидельникова. - Воронеж: Изд-во Воронеж, гос. ун-та, 1996. - 277 с. https://nsau.edu.ru/file/2524/

Попова Т.Н. Изоцитратдегидрогеназы: формы, локализация, свойства и регуляция/Т.Н. Попова//Биохимия. - 1993. - Т . 58, № 126. - С. 1861-1879. https://www.dissercat.com/content/rol-protsessov-svobodnogo-okisleniya-dykhatelnykh-substratov-v-metabolizatsii-zhirnykh-kislo

Cytosolic NADP+ -dependent isocitrate dehydrogenase status modulates oxidative damage to cells / S.M. Lee [et al] // Free Radic. Biol. Med. - 2002. - Vol. 32, № 11.-P. 1185-1196. https://livrepository.liverpool.ac.uk/3004291/1/2016_GBM%20long%20term%20outcome%20(final).docx

Lee S.M. Inactivation NADP+ -dependent isocitrate dehydrogenase by reactive oxygen species / S.M. Lee, T.L. Kil, J.Park // J. Biol. Chem. - 2001. - Vol. 83, № 11-12.-P. 1057-1065.

Lee S.H. et.al. Role of NADP+-dependent isocitrate dehydrogenase (NADP+-ICDH) on cellular defence against oxidative injury by gamma-rays.] // Int. J. Radiat. Biol. - 2004. - Vol. 80, № 9. - P. 635-642. https://pubmed.ncbi.nlm.nih.gov/15586883/

Xiang Xu. et al. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity / // J. Biol. Chem. - 2004. - Vol. 279, № 32. - P. 33946-33957. https://www.jbc.org/article/S0021-9258(20)77462-4/fulltext

Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase / S.H. Jo [et al.] // J. Biol. Chem. - 2001. - Vol. 276, № 19. - P. 16168-16176. https://pubmed.ncbi.nlm.nih.gov/11278619/

Kim H.J. Cellular defense against heat shock-induced oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase / H.J. Kim, B.S. Kang, J.W. Park. // Free Radic. Res. - 2005. - Vol. 39, № 4. - P. 441-448. https://pubmed.ncbi.nlm.nih.gov/16028369/

Lee J.H. Oxalomalate regulates ionizing radiation-induced apoptosis in mice / J.H. Lee , J.W. Park // Free Radic. Biol. Med. - 2007. - Vol. 42, № 1. - P. 44-51. https://europepmc.org/article/med/17350954

Kil I.S. et. al. Mitochondrial NADP+-dependent isocitrate dehydrogenase protects cadmium-induced apoptosis. // Mol. Pharmacol. - 2006. - Vol. 70, №3.-P.1053-1061. https://pubmed.ncbi.nlm.nih.gov/16785314/

Lee J.H. et al. Regulation of ionizing radiation-induced apoptosis by mitochondrial NADP+-dependent isocitrate dehydrogenase. // J. Biol. Chem. — 2007. - Vol. 282, № 18. - P. 13385-13394. https://pubmed.ncbi.nlm.nih.gov/17350954/

Shin S.W. Silencing of mitochondrial NADP+-dependent isocitrate dehydrogenase by small interfering RNA enhances heat shock-induced apoptosis / 171 S.W. Shin, I.S. Kil, J.W. Park // Biochem. Biophys. Res. Commun. - 2008. - Vol. 366, № 4 . - P . 1012-1018. https://pubmed.ncbi.nlm.nih.gov/18096511/

Cox G.F. Nicotinamideadenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria / G.F. Cox, D.D. Davies // Biochem. J. — 1967. - Vol. 105, № 2. - P. 729-734. https://pubmed.ncbi.nlm.nih.gov/4292878/

Прохорова М.И. Методы биохимических исследований / М.И. Прохорова. - СПб.: Изд-во ЛГУ, 1982. - 272 с. https://monographies.ru/en/book/section?id=736

Duggleby R.G. Nicotinamideadenine dinucleotide-specific isocitrate dehydrogenase from higher plant / R.G. Buggleby, D.T. Dennis // J. Biol. Chem. - 1970. - Vol. 245, № 15. - P. 3745-3750. https://www.jbc.org/article/S0021-9258(18)62914-X/abstract

Porpacxy Z. Interection between NAD dependent isocitrate dehydrogenase, a-Ketoglutarate dehydrogenase complex, and NADH: ubiquinion oxidoreductase / Z. Porpacxy, B. Sumegi, I. Alkonyi // J. Biol. Chem. - 1987. - Vol. 262, № 2 0 . - P. 9509-9514

Robinson J.B. Organization of krebs tricarboxylic acid cycle enzymes inmitochondria / J.B. Robinson, P.A. Srere // J. Biol. Chem. - 1985. - Vol. 260, № 19.-P. 10800-10805. https://www.sciencedirect.com/science/article/pii/S0021925819851530

Попова Т.Н. Изоцитратдегидрогеназы высших растений: Формы, локализация, свойства и регуляция : Дис. . д-ра биол. наук: 03.00.04 / Т.Н. Попова Воронеж, 1994. 338с. https://www.dissercat.com/content/regulyatsiya-aktivnosti-nad-i-nadf-izotsitratdegidrogenaz-v-pecheni-krys-pri-induktsii-apopt

Полторак О.М. Физико-имические основы ферментативного катализа / О.М. Полторак, Е.С. Чухрай. - М.: Высшая школа, 1971. - 311 с. https://urss.ru/cgi-bin/db.pl?lang=Ru&blang=ru&page=Book&id=274330

Попова Т.Н. Изоцитратдегидрогеназы: формы, локализация, свойства и регуляция/Т.Н. Попова//Биохимия. - 1993. - Т. 58, № 126. - С. 1861-1879. https://biochemistrymoscow.com/ru/archive/1993/58-12/

Shakhmurova, G.A., Mamadalieva, N.Z., Zhanibekov, A.A. et al. Effect of total ecdysteroid preparation from Silene viridiflora on the immune state of experimental animals under normal and secondary immunodeficiency conditions. Pharm Chem J 46, 222–224 (2012). https://doi.org/10.1007/s11094-012-0766-4

Макеева А.В. Исследование воздействия тиоктовой кислоты на свободнорадикальныи гомеостаз в тканях крыс при патологиях, сопряженных с оксидативным стрессом: автореф. дисс. ... канд. биол. наук / А.В. Макеева. — Воронеж, 2007. - 23 с. http://www.dslib.net/bio-ximia/issledovanie-vozdejstvija-tioktovoj-kisloty-na-svobodnoradikalnyj-gomeostaz-v.html

Shakhmurova, G.A., Syrov, V.N. & Khushbaktova, Z.A. Immunomodulating and antistress activity of ecdysterone and turkesterone under immobilization-induced stress conditions in mice. Pharm Chem J 44, 7–9 (2010). https://doi.org/10.1007/s11094-010-0387-8

Андреещева Е. М. Свойства и регуляция активности аконитатгидратазы и НАДФ-изоцитратдегидрогеназы в условиях активации свободнорадикального окисления а печени крыс при токсическом гепатите: дис. на соискание уч. степ. к. б. н. / Е. М. Андреещева. - Воронеж, 2003.- 273 с. https://www.dissercat.com/content/regulyatsiya-aktivnosti-nad-i-nadf-izotsitratdegidrogenaz-v-pecheni-krys-pri-induktsii-apopt

Левенкова М.В. Глюкозо-6-фосфатдегидрогеназа, свойства и регуляция активности глюкозо-6-фосфатдегидрогеназы в условиях оксидативного стресса при токсическом поражении печени крыс: автореф. дисс. ... канд. биол. наук / М.В. Левенкова. - Воронеж, 2006. - 22 с. https://www.dissercat.com/content/svoistva-i-regulyatsiya-aktivnosti-6-fosfoglyukonatdegidrogenazy-v-usloviyakh-oksidativnogo-

IDH1 gene transcription is sterol regulated and activated by SREBP-la and SREBP-2 in human hepatoma HepG2 cells: evidence that IDH1 may regulate lipogenesis in hepatic cells / I. Shechter [et. al.] // J. Lipid Research. - 2003. - Vol. 44.-P. 2169-2180. https://pubmed.ncbi.nlm.nih.gov/12923220/

N.N. Karkishenko, D.B. Chayvanov, A.A. Vartanov. On the effectiveness and safety of ultrasonic transcranial stimulation of the human brain. [Ob jeffektivnosti i bezopasnosti ul'trazvukovoj transkranial'noj stimuljacii golovnogo mozga cheloveka] // Biomedical Journal. 2011. No. 2, pp.4-17. https://journal.scbmt.ru/jour/article/view/635

Tuychieva, D., Mirkhamidova, P., Alimova, R., Shakhmurova, G. Influence of drop on the ultrastructure lipid composition of liver hepatocytes of pregnant rats. //BIO Web of Conferences, 2024, 113, 02021. https://www.bio-conferences.org/articles/bioconf/abs/2024/32/bioconf_interagromash2024_02021/bioconf_interagromash2024_02021.html

Babakhanova, D., Mirkhamidova, P., Alimova, R., Shakhmurova, G., Mukhamedov, G. Effects of ultrasound waves on rat liver mitochondria //BIO Web of Conferences, 2024, 100, 01005. https://doi.org/10.1051/bioconf/202410001005

Mirkhamidova, P., Shakhmurova, G. Determination of the activity of some antioxidant enzymes during the germination periods of cereals and legumes //E3S Web of Conferences 452, 01018(2023). https://doi.org/10.1051/e3sconf/202345201018

Tuychieva, D., Mirkhamidova, P., Shakhmurova, G.A., Zhumagulova, K.A. The effect of some pesticides on lipid peroxidation and on the content of cytochrome p-450 of mitochondrial membranes and liver microsomes of pregnant rats and their embryos // E3S Web of Conferences 452, 01017 (2023). https://doi.org/10.1051/e3sconf/202345201017

Schneider W.C., Hageboom G.H., Pallade G.E. Cytochemical studies of mammalian tissues; isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate material // J. Biol. Chem. – 1948. – V. 172 (2). – P.619-635

Кузмина С.Н., Карандашвиле Ф.А, Бульдеяева Т.В./ Цитохромоксидаза ядерных оболочек печени крыс и её взаимоотношение в митохондриальной цитохромоксидазей// Биохимия. – 1976. – Т.41.-С.1684-1697

Рогожин В.В. Практикум по биохимии. Учебное пособие. Санкт-Петербург. Изд.: «Лань» 2013. С. 351-353

Lowry H., Rosenbrouch H.G., Farr A.L., Randall R. Protein measurement with the folin phenol reagent // J. Biol. Chem. – 1975. – V.193(№1) – P. 265-275.

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2025-05-02

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1.
Mukhamedov G, Babakhanova D, Mirkhamidova P, Alimova R, Shakhmurova G, Eshonkulov O. Influence Of Ultrasonic Waves on The Activity of Above and Nadp - Dependent Liver Isocytedehydrogenase in Rats and Ways of Their Correction. J Neonatal Surg [Internet]. 2025 May 2 [cited 2026 Apr. 1];14(20S):443-9. Available from: https://jneonatalsurg.com/index.php/jns/article/view/5039

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Vol. 14 No. 20S (2025): Journal of Neonatal Surgery

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Original Article

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