Investigation the effect of isotretinoin on ovary tissues and reproduction in female rats
DOI:
https://doi.org/10.52783/jns.v14.3732Keywords:
Isotretinoin, acne vulgaris, ovaries, teratogenic agentAbstract
Background: Oral isotretinoin, a potent synthetic retinoid, derived from vitamin A, uniquely eradicates acne vulgaris. Its multifaceted action on skin health is attributed to nuclear and non-nuclear receptor transactivation. However, isotretinoin's teratogenicity and associated serious adverse effects necessitate careful consideration, particularly regarding its use during pregnancy, where a notable incidence of congenital anomalies and spontaneous abortion has been documented. Isotretinoin is regarded as a highly teratogenic agent. may adversely affect ovarian reserve and female fertility, evidenced by decreased anti-mullerian hormone (AMH) levels. .
Objective: This study aims to elucidate the detrimental effects of toxic isotretinoin doses on reproductive efficacy by investigating its influence on reproductive organs tissue and reproduction.
Materials and methods: forty rats were used in this study, The current study consists of two parts each part used 20 rats which lasted for month. each group contain 5 rats. The first part: Consists of four groups, The first group was the control group, while the second group was given the drug 15 mg/kg isotretinoin and the third group was given 30 mg/kg isotretinoin. The fourth group was given 30 mg/kg isotretinoin and omega-3 for 30 days. the blood samples of all rats were obtained by cardiac puncture under the anesthesia for Assessment of histopathological ovaries for Rat. Then, the rats were sacrificed, and bilateral ovariectomy was performed ovarian tissues were fixed in a 10% formalin solution for histological evaluation. It was noticed after a histological exam in groups 3, and 4 that the ovarian atrophied mucosal with degeneration of epithelial cells cortex contained atrophied ovarian follicles with degenerated Oocytes, the medulla of the ovary contained congested blood vessels hemorrhage in the interstitial connective tissue of the medulla also hemolysis blood of blood capillaries While the second part, the second part was to evaluate the effect of the drug on reproductive efficiency. Four groups were divided, each group containing five rats, and were given the same dose for a month. After treatment, a male was introduced to each group and left to mate with the females. The result was pregnancy and births only in the control group, while no pregnancy occurred in the other group, The administration of isotretinoin, particularly in elevated doses, poses substantial risks to female reproductive efficacy, notably affecting ovarian reserve, a key fertility determinant.
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