Histomorphological And Histochemical Study Of Pituitary Gland In Adults Male New Zealand Rabbits
DOI:
https://doi.org/10.52783/jns.v14.3730Keywords:
pituitary, morphology, Rabbit, AdenohypophysisAbstract
The present study aimed to examine the morphology, histology, and histochemistry of the pituitary gland in adult male rabbits. Ten adult male rabbits were used, and samples from their pituitary glands were collected. The tissue specimens were processed and stained with Hematoxylin and Eosin (H&E), with an additional special stain (Orange G) applied. Morphological observations were made regarding the shape and location of the pituitary gland. Histologically, the pituitary gland was encased in a thin capsule derived from the dura mater, tightly surrounding the gland. The results revealed that the pituitary gland(hypophysis) was primarily composed of the adenohypophysis and neurohypophysis, with the adenohypophysis being the main component. The adenohypophysis included the pars distalis, pars intermedia, and pars tuberalis. The pars distalis was located laterally, while the pars nervosa was situated at the center of the gland. Additionally, the pars distalis contained three main cell types: acidophils, basophils, and chromophobes. The H&E staining results highlighted that acidophils were the most prominent cells. The cellular structure of the pars distalis consisted of irregularly arranged clusters and cords of chromophilic and chromophobic cells, mainly concentrated at the periphery of the pars distalis
Downloads
Metrics
References
Adams C, Swettenham K (1958). The histochemical identification of two types of basophil cell in the normal human adenohypophysis. J. Pathol. Bacteriol., 75: 95–103. https://doi.org/10.1002/path.1700750111
Al-Dbag TA (1998). Vascular pathways of the pituitary gland in donkeys. The Iraqi J. Vet. Med., 22(1): 185–191. https://doi.org/10.30539/ijvm.v22i1.1217
AL-Mahdawi RIK, Yakoob AY, abd Alsattar R (2015). Study of histopathological changes in brain of mice infected with Toxoplasma gondii isolated from domestic rabbit. The Iraqi J. Vet. Med., 39(1): 90–93.
Alnoor S, Al-Jibbori N, Mehdi AWR (2024). Structural-functional aspects of the pineal, pituitary, ovary and uterus of Trypanosoma evansi inoculated rabbit. The Iraqi J. Vet. Med., 12(1): 255–274. https://doi.org/10.30539/cgrh7h29
AL-Okaily BN, Al-Haddad AA, Salman AD (2013). Effect of different doses of doxorubicin on pituitary gland and some testicular function in adult male rabbits.The Iraqi J. Vet. Med., 37(1): 121–128. https://doi.org/10.30539/iraqijvm.v37i1.344
Al-Zubaidiy KAK (2012). Anatomical and histological study of pituitary gland and testis in fetal, newborn and adult guinea pig (Cavia porcellus). Ph.D. Thesis, Coll. Vet. Med., Univ. Baghdad, Iraq.
Delmann HD (1993). Endocrine system. In Textbook of veterinary histology, 4th ed., (HD Dellmann, ed.), Lea & Febiger, Philadelphia, pp. 270–284.
Dyce KM, Sack WO, Wensing CJG (1996). Veterinary anatomy. 2nd ed. W.B. Saunders Co., Philadelphia.
El-Sakhawy M, Ghareeb N, ElBargeesy G, Rahman A (2012). Post-hatching development of the pars distalis of the adenohypophysis of the quail (Coturnix coturnix). J. Vet. Anat., 5: 1–15.
Flesh M (1884). Ueber den Bau der Hypophyse des Pferdes. Ges. Dtsch. Naturf. Aerzte, 57: 195.
Gómez-Domínguez EG, Toriz CG, González-Pozos S, González-del-Pliego M, Aguirre-Benítez EL, Pérez-Torres A, Flores-Martinez YM, Solano-Agama C, Rodríguez-Mata V, García-Godínez A, Martínez-Fong D, Mendoza-Garrido ME (2023). Characterization of the rat pituitary capsule: evidence that the cerebrospinal fluid filled the pituitary cleft and the inner side of the capsule. PLoS ONE, 18(5): e0286399.
Greaves P (2007). Endocrine glands. In Histopathology of preclinical toxicity studies (pp. 782–795). Academic Press.
Herring PT (1908). The histological appearances of the mammalian pituitary body. Q. J. Exp. Physiol., 1: 149–153.
Hussein AA, Dahl MO, Mustafa NG (2024). Biochemical parameters of laboratory animals in Iraq: a meta-analysis study. The Iraqi J. Vet. Med., 48(2): 8–14. https://doi.org/10.30539/te4r3w32
Hussein BM (2015). Histological study of pars tuberalis of the pituitary gland of rats in Iraq. J. Kerbala Univ., 13(1): [page-range].
Ibrahim S, El-Shawarby A, Soliman A (2002). Age related changes in thyrotrophs of the anterior pituitary gland and the thyroid gland of male albino rats: histological, immunocytochemical and biochemical study. Egypt. J. Histol., 25: 41–56.
Ismail A, Atyia MA (2013). Morphological changes of descending testes during postnatal developmental stages in the rabbit (Oryctolagus cuniculus). The Iraqi J. Vet. Med., 37(2): 237–243. https://doi.org/10.30539/ijvm.v37i2.1386
Karunasri V, Nagamalleswari Y, Kishore PVS, Raghunath M (2015). Gross and histological study on the pars distalis of pituitary gland of adult male Sprague Dawley rat. Indian J. Vet. Anat., 27(2): 67–69.
Kelberman D, Rizzoti K, Lovell-Badge R, Robinson ICAF, Dattani MT (2009). Genetic regulation of pituitary gland development in human and mouse. Endocrinol. Dev., 30(7): 790–829.
Khon A (1910). Ueber die Hypophyse. Munch. Med. Wochenschr., 57: 1485.
Kovacs TEH, Hovarth E, Ryan N (1981). Immunocytology of human pituitary. In Diellis RA, ed. Diagnostic immunocytochemistry. Masson Publishing, New York.
Luay OH (2016). Some macroscopic and microscopic observation on the pituitary gland of guinea-pig (Cavia culteri). Bas. J. Vet. Res., 15(4): [page-range].
Malallah HB, Hussin AM (2010). Histological study of pars tuberalis of the pituitary gland in local male buffaloes (Bubalus bubalis). The Iraqi J. Vet. Med., 34(2): 99–102. https://doi.org/10.30539/iraqijvm.v34i2.637
Manojlović-Stojanoski M, Nestorović N, Negić N, Filipović B, Sosić-Jurjević B, Sekulić M, Milosević V (2007). Influence of maternal dexamethasone treatment on morphometric characteristics of pituitary GH cells and body weight in near-term rat fetuses. Folia Histochem. Cytobiol., 45(1): 51–56.
Mapara M, Thomas BS, Bhat KM (2012). Rabbit as an animal model for experimental research. Dent. Res. J. (Isfahan), 9(1): 111–118.
Murakami M, Yoshida T, Nakayama Y, Hashimoto J, Hirata S (1968). The fine structure of the pars intermedia of the pituitary in the human fetus. Arch. Histol. Jpn., 30: 61–73.
Oishi Y, Matsumoto M, Yoshizawa K, Tsubura A, Morii S (1992). Spontaneous pituitary adenomas of the pars intermedia in mice and rats: histopathological and immunocytochemical studies. J. Toxicol. Pathol., 5: 223–231.
Osamura RY (1983). Pituitary gland, rat. In Jones TC, Mohr U, Hunt RD, Capen CC (eds). Endocrine system. Monographs on pathology of laboratory animals. Springer, Berlin.
Pearse AG (1968). Histochemistry, theoretical and applied. Vol. I. J. & A. Churchill Ltd., London.
Rasmussen AT (1924). A quantitative study of the human hypophysis cerebri, or pituitary body. Endocrinology, 8: 509–524.
Ross MH, Reith EJ, Romrell LJ (1989). Histology: a text and atlas. 2nd ed. Williams & Wilkins, Baltimore.
Scott IS, Chattopadhyay A, Ansorge O (2024). Development and microscopic anatomy of the pituitary gland. In Feingold KR, Anawalt B, Blackman MR, et al., eds. Endotext [Internet]. MDText.com, Inc., South Dartmouth (MA). Sep 28, 2024.
Trautman A (1909a). Die makroskopischen Verhaltnisse der Hypophyse einiger Sauger. Arch. Wiss. Prakt. Tierheilkd., 35: 614–637.
Trautman A (1909b). Anatomie und Histologie der Hypophysis Cerebrieiniger Sauger. Arch. Mikrosk. Anat., 74: 311–367.
Tucker MJ (1999). Pituitary toxicology: Direct toxicity, secondary changes and effects on distal target tissues. In Harvey PW, Rush KR, Cockburn A, eds. Endocrine and hormonal toxicology (pp. 15–32). Wiley, Chichester.
Turner CD, Bagnara JT (1976). General endocrinology. 6th ed. W.B. Saunders, Philadelphia.
Vinson GP, Whitehouse BJ, Bateman A, Hrudy VJ, Sawyer TK, Darman PS (1984). α-MSH analogues and adrenal zona glomerulosa function. Life Sci., 35: 603–610
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.