Age estimation based on tooth cementum annulations using light and phase contrast microscope
DOI:
https://doi.org/10.52783/jns.v14.3292Keywords:
light microscope, phase contrast microscope, cementum annulations, age estimationAbstract
Background: This study aimed to compare the estimated age with the actual age by analysing cementum incremental lines using light and phase contrast microscopy
Methodology: Conducted in the Department of Oral and Maxillofacial Pathology and Oral Microbiology at Maharishi Markandeshwar (Deemed to be University) Mullana, Ambala, the study utilized 40 extracted teeth (20 males and 20 females), that were stored 10% formalin. Longitudinal ground sections were prepared by using a lathe - cutting machine followed by hand-trimming on the Arkansas stone. These ground sections were mounted with DPX and examined under both light and phase contrast microscopes simultaneously then these slides were transferred to the computer monitor from microscopes under 40x magnification by using NIS Element software. Cementum displayed alternating dark and bright bands, each pair representing a single annulation was counted as one and added at the end with tooth eruption age to estimate the actual age of an individual.
Results: The connection between the actual age and phase-contrast microscopy readings was stronger (r = 0.938, p = 0.0001) compared to light microscopy (r = 0.677, p = 0.0001). In male subjects the phase contrast microscopy showed slight underestimation of age (actual: 25.20, estimated: 23.20, p = 0.001), whereas light microscopy understated age even more (actual: 25.20, estimated: 20.75, p = 0.005). Phase contrast microscopy revealed no variation of age in females (actual: 18.80, estimated: 18.85, p = 0.942), but light microscopy revealed understated age (actual: 18.80, estimated: 17.00, p = 0.181). These results suggest that while phase contrast microscopy was more accurate in predicting the actual age, its dependability is impacted by gender variations as we can see more disparities in male samples.
Conclusion: The study confirms the stronger association between the cementum annulations and the chronological age, with phase contrast microscopy showing better accuracy as compared to light microscopy
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Geetha HL, Baghisath VP, Vinay HB, Sudheer B, Kumar VJ, Gayathri CH. Age estimation using tooth cementum annulations method by different types of microscopes: A comparative study. Int J Oral Health Sci 2018; 8:73-80.
Pradeep L, Kokila G, Gopinathan PA, Guruswamy S, Nazir SH, Chatterjee A. Age Estimation with Cemental Annulation Using Light, Phase Contrast and Polarized Microscopy. J Microsc Ultrastruct. 2021; 9:55-60.
Kaur P, Astekar M, Singh J, Arora KS, Bhalla G. Estimation of age based on tooth cementum annulations: A comparative study using light, polarized, and phase contrast microscopy. J Forensic Dent Sci. 2015;7(3):215-21.
Patil A, Satish B N, Kondody RT, Wali V. Cemental annulation: An approach to age estimation in transverse and longitudinal sections using three different microscopes. J Oral Maxillofac Pathol 2022; 26:192-98.
Kulkarni N, Mahesh S, Sridharan G. Dental age estimation using incremental lines of cementum. International Journal of Oral Health Dentistry. 2018;4(3):202-04.
Gupta P, Kaur H, Shankari GSM, Jawanda MK, Sahi N. Human age estimation from tooth cementum and dentin. J Clin Diagn Res. 2014;8(4):ZC07-10.
Hinrichs, C., Nicklisch, N., Mardare, C. C., Orechovski, B., Hassel, A. W., Kleber, C., & Alt, K. W. (2022). Incremental lines in human acellular tooth cementum – New insights by SEM analysis. Anatomischer Anzeiger [Annals of Anatomy], 243(151933), 151933.
Mohan N, Gokulraj S, Thomas M. Age estimation by cemental annulation rings. J Forensic Dent Sci. 2018;10:79-83.
Kagerer P, Grupe G. Age-at-death diagnosis and determination of life-history parameters by incremental lines in human dental cementum as an identification aid. Forensic science international. 2001 Apr 15;118(1):75-82.
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