Phytochemical Characterization and Anti-Acne Potential of Cassia fistula and Plumeria obtusa Extracts Against Cutibacterium acnes
DOI:
https://doi.org/10.52783/jns.v14.2767Keywords:
Acne vulgaris, Phytochemicals, Antibacterial activity, Antioxidant properties, Cassia fistula, Plumeria obtusaAbstract
The Cassia fistula and Plumeria obtusa extracts were evaluated for their anti-acne efficacy against the key pathogen in acne vulgaris, Cutibacterium acnes, with respect to their phytochemical composition. This research explores plant derived, antimicrobial and antioxidant properties with the increasing antibiotic resistance and toxic effects of conventional treatments. Flavonoids, alkaloids, terpenoids, polyphenols, steroids, glycosides were chemical compounds identified in phytochemical analysis of the extracts already known to be bioactive. The bioactive constituent’s resveratrol, cinnamic acid and plumericin along with derivatives from andrographolide received validation through Nano Liquid Chromatography coupled Tandem Mass Spectrometry (Nano LC/MS/MS) analysis. Higher concentration of resveratrol was seen in Plumeria obtusa, which have higher potential for pharmacological activity seen as compared to the Cassia fistula. Plumeria obtusa showed better free radical neutralization (IC50 = 1050.91 µg/mL) than by the DPPH radical scavenging assay. Both extracts have a biocompatibility demonstrated by their cytotoxicity evaluation using Vero cells and then used in dermatological application. Microdilution assays showed that C. acneswas significantly inhibited with Plumeria obtusa having superior activity over Cassia fistula, which were attributed at higher polyphenol and flavonoid content only. These findings lend support for therapeutic use of these plant extracts in acne. Plumeria obtusa, showing better antimicrobial and antioxidant activity than other species, becomes a promising candidate of phyto therapeutic formulation. Additional investigations utilizing in vivo evaluations and development of formulation is then necessary to achieve clinical applicability and to confirm effectively.Cassia fistula and Plumeria obtusa extracts were evaluatedwhich has higher potential. These findings support the therapeutic use of these plant extracts in acne.
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Konstantinou E, Longange E, Kaya G. Mechanisms of Senescence and Anti-Senescence Strategies in the Skin. Biology. 2024;13(9):647.
Firlej E, Kowalska W, Szymaszek K, Roliński J, Bartosińska J. The role of skin immune system in acne. Journal of Clinical Medicine. 2022;11(6):1579.
Qi L, Zhao Q, Guo L, Zhao B, Zhang M. Prevention and Care for Moisture-Associated Skin Damage: A Scoping Review. Journal of Tissue Viability. 2024.
Skroza N, Tolino E, Mambrin A, Zuber S, Balduzzi V, Marchesiello A, et al. Adult acne versus adolescent acne: a retrospective study of 1,167 patients. The Journal of clinical and aesthetic dermatology. 2018;11(1):21.
Xu W, Xu J, Huang D, Wang C, Song J, Chen X, et al. Acne vulgaris: advances in pathogenesis and prevention strategies. European Journal of Clinical Microbiology & Infectious Diseases. 2025:1-18.
McDowell A. Cutibacterium (previously Propionibacterium) acnes and disease. Molecular Medical Microbiology: Elsevier; 2024. p. 881-903.
De La Hoz-Romo MC, Díaz L, Gómez-León J, Quintero M, Villamil L. Marine actinobacteria metabolites: unlocking new treatments for acne vulgaris. Frontiers in Microbiology. 2025;15:1501951.
Leung AK, Barankin B, Lam JM, Leong KF, Hon KL. Dermatology: how to manage acne vulgaris. Drugs in context. 2021;10.
Roller L, Gowan J. Acne: Pimples, zits and spots. AJP: The Australian Journal of Pharmacy. 2024;105(1237):80-7.
Reynolds RV, Yeung H, Cheng CE, Cook-Bolden F, Desai SR, Druby KM, et al. Guidelines of care for the management of acne vulgaris. Journal of the American Academy of Dermatology. 2024;90(5):1006. e1-. e30.
Abubakar AB, Abduljalal AS. The contemporary issues related to conventional treatment of acne and the way forward. Public Health Risk Assesment Journal. 2024;2(1):17-27.
Cros MP, Mir-Pedrol J, Toloza L, Knödlseder N, Maruotti J, Zouboulis CC, et al. New insights into the role of Cutibacterium acnes-derived extracellular vesicles in inflammatory skin disorders. Scientific Reports. 2023;13(1):16058.
Kim HJ, Kim YH. Exploring Acne Treatments: From Pathophysiological Mechanisms to Emerging Therapies. International Journal of Molecular Sciences. 2024;25(10):5302.
Li Y, Hu X, Dong G, Wang X, Liu T. Acne treatment: research progress and new perspectives. Frontiers in Medicine. 2024;11:1425675.
Singh N, Singh A, Pandey K, Nimisha. Current Insights for the Management of Acne in the Modern era. Recent Patents on Anti-Infective Drug Discovery. 2020;15(1):3-29.
Nurzyńska-Wierdak R, Pietrasik D, Walasek-Janusz M. Essential oils in the treatment of various types of acne—a review. Plants. 2022;12(1):90.
Chauhan PN, Sharma A, Rasheed H, Mathur H, Sharma P. Treatment opportunities and technological progress prospective for acne vulgaris. Current drug delivery. 2023;20(8):1037-48.
Li MK, Liu C, Hsu JT. The use of lasers and light devices in acne management: an update. American Journal of Clinical Dermatology. 2021;22:785-800.
Patra JK, Das G, Lee S, Kang S-S, Shin H-S. Selected commercial plants: A review of extraction and isolation of bioactive compounds and their pharmacological market value. Trends in Food Science & Technology. 2018;82:89-109.
Majolo F, Delwing LKdOB, Marmitt DJ, Bustamante-Filho IC, Goettert MI. Medicinal plants and bioactive natural compounds for cancer treatment: Important advances for drug discovery. Phytochemistry Letters. 2019;31:196-207.
Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, et al. Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. BioMed Research International. 2022;2022(1):5445291.
Bungau AF, Radu A-F, Bungau SG, Vesa CM, Tit DM, Purza AL, et al. Emerging insights into the applicability of essential oils in the management of acne vulgaris. Molecules. 2023;28(17):6395.
Uhlířová R, Langová D, Bendová A, Gross M, Skoumalová P, Márová I. Antimicrobial activity of gelatin nanofibers enriched by essential oils against Cutibacterium acnes and Staphylococcus epidermidis. Nanomaterials. 2023;13(5):844.
Li L, He Y, Zou Q, Chen W, Liu Y, He H, et al. In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts. Frontiers in Microbiology. 2024;15:1456240.
Devi P, Kumar P. Challenges in the Storage of Herbal Medicine Products and Strategies for Sustainable Management. Herbal Medicine Phytochemistry: Applications and Trends: Springer; 2024. p. 1739-67.
Salau A, Shehu M, Bakare-Odunola M. Navigating the Challenges of Integrating African Herbal Medicines: A Path to Universal Acceptance: Herbal Medicines. Fountain Journal of Natural and Applied Sciences. 2024;13(1).
Chugh NA, Bali S, Koul A. Integration of botanicals in contemporary medicine: road blocks, checkpoints and go-ahead signals. Integrative medicine research. 2018;7(2):109-25.
Ferreira I, Lopes CM, Amaral MH. Treatment advances for acne vulgaris: the scientific role of cannabinoids. Cosmetics. 2024;11(1):22.
Villa SM, Heckman J, Bandyopadhyay D. Medicinally privileged natural chalcones: Abundance, mechanisms of action, and clinical trials. International journal of molecular sciences. 2024;25(17):9623.
Praveen N, Preetha RST, Pagare V, Devasia J, Nizam A, Mukherjee E, et al. Plant-based Metabolites as Source of Antimicrobial Therapeutics: Prospects and Challenges. Antimicrobials in Pharmaceutical and Medicinal Research. 2023:165-201.
Chudasama R, Dhanani N, Amrutiya R, Chandni R, Jayanthi G, Karthikeyan K. Screening of selected plants from semi-arid region for its phytochemical constituents and antimicrobial activity. Journal of Pharmacognosy and Phytochemistry. 2018;7(2):2983-8.
Rabadia JP, Desai TR, Thite VS. Plumeria acuminata: A Systematic In Vivo Evaluation for its Antiovulatory and Anti-implantation Features. Current Drug Therapy. 2022;17(3):186-207.
Mwangi RW, Macharia JM, Wagara IN, Bence RL. The medicinal properties of Cassia fistula L: A review. Biomedicine & Pharmacotherapy. 2021;144:112240.
Arunachalam N, Jayasankar S, Rajendran J, Thamilarasan R. Phytochemicals, Anti-microbial and Anti-oxidant Investigations on Cassia Fistula Fruit Pulp Extracts. Biomedical and Pharmacology Journal. 2024;17(4):2515-25.
Bihani T, Tandel P, Wadekar J. Plumeria obtusa L.: A systematic review of its traditional uses, morphology, phytochemistry and pharmacology. Phytomedicine Plus. 2021;1(2):100052.
Souza BdS, Sales ACS, Moita LA, Oliveira NVdM, Silva FDSd, Barbosa MS, et al. Plumeria species: a review of morphology, traditional uses, phytochemicals, and pharmacological activities. 2024.
Singh S, Saha P, Rai N, Kumari S, Pandey-Rai S. Unravelling triterpenoid biosynthesis in plants for applications in bioengineering and large-scale sustainable production. Industrial Crops and Products. 2023;199:116789.
Anggoro B, Istyastono E, Hariono M. Future molecular medicine from white frangipani (Plumeria alba L.): A review. Journal of Medicinal Plants Research. 2020;14(10):544-54.
Tahami SR, Nemati NH, Keshvari H, Khorasani MT. In vitro and in vivo evaluation of nanofibre mats containing Calendula officinalis extract as a wound dressing. Journal of Wound Care. 2022;31(7):598-611.
Kumari D, Singh D, Meena M, Janmeda P, Siddiqui MH. Qualitative, Quantitative, In Vitro Antioxidant Activity and Chemical Profiling of Leptadenia pyrotechnica (Forssk.) Decne Using Advanced Analytical Techniques. Antioxidants. 2024;13(7):794.
Ali M, Wahab M, Ahmad L, Ahmad I, Semotiuk AJ, Jan HA. Ethnopharmacological evaluation of medicinal plants used to treat diabetes mellitus in Maidan valley, Dir Lower, Pakistan. Natural and Applied Sciences International Journal (NASIJ). 2022;3(1):45-60.
Sultana P, Sarker AK. Phytochemical and antioxidant potential of the crude extracts of Ipomoea quamoclit. development. 2023;10:11.
Sharma RK, Sharma N, Kumar U, Samant SS. Antioxidant properties, phenolics and flavonoids content of some economically important plants from North-west Indian Himalaya. Natural Product Research. 2022;36(6):1565-9.
Patil-Patankar V. Comparative pharmacognostic exploration of medicinal plants and their adulterants. Herbal Formulations, Phytochemistry and Pharmacognosy: Elsevier; 2024. p. 199-217.
Lewis I, James S. Performance standards for antimicrobial susceptibility testing. (No Title). 2022.
Cordeiro M, Nunes T, Bezerra F, Damasco P, Silva W, Ferreira M, et al. Phytochemical characterization and biological activities of Plectranthus barbatus Andrews. Brazilian Journal of Biology. 2021;82:e236297.
Baquer F, Ali Sawan A, Auzou M, Grillon A, Jaulhac B, Join-Lambert O, et al. Broth microdilution and gradient diffusion strips vs. reference agar dilution method: first evaluation for Clostridiales species antimicrobial susceptibility testing. Antibiotics. 2021;10(8):975.
Zafar S, Faisal S, Jan H, Ullah R, Rizwan M, Abdullah, et al. Development of iron nanoparticles (FeNPs) using biomass of enterobacter: Its characterization, antimicrobial, anti-Alzheimer’s, and enzyme inhibition potential. Micromachines. 2022;13(8):1259.
Abdullah ATM, Rahman MM, Sharif M, Khan TA, Islam SN. Bioactive polyphenolic compounds and antioxidant potentials of two leafy vegetables in Bangladesh: the Momordica charantia and the Ipomoea aquatica. Food Production, Processing and Nutrition. 2024;6(1):35.
Vishwakarma A, Shrivastava K. Comparative Study Of Phyllanthus Niruri, Sphaeranthus Indicus And Tridax Procumbens With Respect To Qualitative & Quantitative Estimation Of Phytochemicals. Journal of Advanced Zoology. 2024;45(2).
Arora M, Arora K, Kaur R. Pharmacognostic, physicochemical, phytochemical, nutraceutical evaluation and in vitro antioxidant potency of Habenaria intermedia D. Don—A rare orchid. South African Journal of Botany. 2023;152:278-87.
Sadalage NA, Baidya M, Kondrapu P, Ghiware NB, Shaha AP, Mayur S, et al. PHYTOCHEMICAL EVALUATION, IN VITRO ANTIOXIDANT ACTIVITY AND IN-VIVO ANTIDIABETIC ACTIVITY OF ACACIA NILOTICA.
Mishra Y, Yadav RR, Singh AK, Verma K, Yadav N. A Comparative Analysis for Phytochemical Screening and Antioxidant Potential of Two Different Formulations of Triphala Extract. Journal of Ecophysiology and Occupational Health. 2023:343-50.
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