Green Synthesis of Silver Nanoparticles Using Persea Americana Fruit Extract Prevents Adipogenesis of 3t3-L1 Cells Via Down Regulating Ppar-γ

Abstract

Obesity is one of the major public health concerns. In the last two decades, Obesity and its comorbidities were witnessed among the people worldwide which making fifth main cause of human mortality. Excessive weight gain occurs due to sedentary life style, imbalance in utilization and consumption of calories, and intake of high energy dense food rich in high sugar and fat content, that leads to onset of several health issues such as Type II diabetes, cancer, cardio vascular diseases, respiratory diseases, and Musculo skeletal disorders. Various synthetic drugs are available in the market for the treatment of obesity, but on the other side these are accompanied with inimical effects. To overcome this problem, and in the view of current scenario, emphasizing more on the development of natural bioactive products for the obesity management. However, experimental evidence is required to support obesity management mechanisms or unveil new ones. Hence silver nanoparticles were prepared from Persea americana and its anti-obesity mechanisms were tested with nuclear receptors called PPARY gamma which plays a significant role on adipogenesis and fat metabolism.

The silver nanoparticles were prepared from the extract of Persea americana and it is encapsulated with Orlistat which is a standard drug used as positive control. The nanoparticles were characterized physically and spectroscopically. The viability of nanoparticles was assessed with 3T3L1 cell line by MTT assay. The lipid content was estimated in 3T3L1 cell line by oil-O-red staining method. The level of triglycerides was estimated on 3T3L1 cell line. The impact of the Persea americana silver nanoparticle and Orlistat encapsulated silver nanoparticles on anti-adipogenic effect by using epigenetic markers with 3T3L1 cell lines.