Serological Investigation of Human Brucellosis in Pyrexia of Unknown Origin Patients
DOI:
https://doi.org/10.52783/jns.v14.2015Keywords:
RBPT- Rose Bengal Plate test, SAT-Serum Agglutination Test, ELISA IgM- Enzyme linked immunosorbent assay Immunoglobulin M, ELISA IgG- Enzyme linked immunosorbent assay Immunoglobulin G, PUO- Pyrexia of unknown originAbstract
Introduction: The objective of our study was to assess the seroprevalence of brucellosis though the different serological tests. Brucellosis is the one of the major global zoonoses that affects many nations, including India.
Material and Methods: The study period for this study was two years and samples were collected from the patients attending different wards at a tertiary care hospital King George’s Medical University, Lucknow in Uttar Pradesh, Northern India. The study design for this study was hospital based. Serum samples from 275 distinct cases of Pyrexia of Unknown Origin (PUO) were collected. Rose-Bengal plate test (RBPT), serum agglutination test (SAT), ELISA IgM and ELISA IgG, were used for the serological analysis of the samples.
Results: Brucella antibodies were detected in 70 (25.45%), 72 (26.18%), 31 (11.27%) and 5 (1.8%) of sera by RBPT, SAT for B. abortus, SAT for B. melitensis, ELISA IgM and ELISA IgG, respectively.
Conclusion: Risk factors such as contact with animals and animal products were significantly linked with brucellosis. Further epidemiology studies are warranted in such regions of endemicity to determine accurate estimates of prevalence and risk factors and to study Brucella biovars for appropriate policymaking and advocacy and awareness regarding brucellosis in Northeast India.
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