An In Vitro Comparative Study on the Bond Strength of BioRoot RCS and MTA-Based Sealers with Regenerative Endodontic Blood Clot Scaffold

Authors

  • Prashant Ashok Shelar
  • M. Robert Justin
  • Aditi Subhashchandra Shede
  • Himanshu Srivastava
  • Aniket Gulab Pawar

Keywords:

BioRoot RCS, MTA-Based Sealer, Regenerative Endodontics, Blood Clot Scaffold, Push-Out Bond Strength.

Abstract

The success of regenerative endodontic procedures depends on the establishment of a durable seal between the root canal filling material and dentinal walls while preserving the biological environment created by the blood clot scaffold. The present in vitro study was conducted to compare the bond strength of BioRoot RCS and MTA-based sealers when used in conjunction with a regenerative endodontic blood clot scaffold. Forty extracted human single-rooted teeth were standardized, instrumented, and divided into two groups according to the sealer used. A regenerative blood clot scaffold was established in all specimens before obturation. Group I was obturated using BioRoot RCS, whereas Group II was obturated using an MTA-based sealer. After storage under controlled conditions, root sections were subjected to push-out bond strength testing using a universal testing machine. Failure modes were further evaluated microscopically. The findings demonstrated successful scaffold formation and satisfactory obturation quality in all specimens. BioRoot RCS exhibited higher bond strength values and greater resistance to dislodgement than the MTA-based sealer. Mixed failure was the predominant mode observed in the BioRoot RCS group, while adhesive failure occurred more frequently in the MTA-based sealer group. Within the limitations of this in vitro investigation, BioRoot RCS demonstrated superior bonding performance and adaptability in the presence of a regenerative endodontic blood clot scaffold......

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References

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Published

2025-12-12

How to Cite

1.
Ashok Shelar P, Justin MR, Shede AS, Srivastava H, Pawar AG. An In Vitro Comparative Study on the Bond Strength of BioRoot RCS and MTA-Based Sealers with Regenerative Endodontic Blood Clot Scaffold. J Neonatal Surg [Internet]. 2025 Dec. 12 [cited 2026 Sep. 12];14(33S):1474-86. Available from: https://jneonatalsurg.com/index.php/jns/article/view/10553

Issue

Vol. 14 No. 33S (2025): Journal of Neonatal Surgery

Section

Original Article

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