Formulation Development and Evaluation of Nanoemulsion of Hippaestrum vittatum (Lily) flowers extract
Keywords:
Lily, nanoemulsion, spontaneous emulsification method, drug release...Abstract
Due to their capacity for regulated distribution and optimal dispersion of active substances into the targeted layers of skin, cosmeceutical nanoemulsions for skin care are in higher demand. The current study was based on the formulation development and evaluation of nanoemulsion of Hippaestrum vittatum. The flowers of H. vittatum were collected from the Unnao region, Uttar Pradesh. These were identified and authenticated by a botanist. These were washed, dried under shade and sieved for making dust-free and kept at room temperature. The powder of Hippaestrum vittatum were weighed and extracted through maceration using ethyl alcohol. The flowers of Hippaestrum vittatum were soaked into a beaker containing ethyl alcohol solvent for 15 days with frequent stirrings. The beaker was mounted with aluminium foil. Preliminary phytochemical screening was done for the herbal extract. After preformulation study, nanoemulsion was prepared using spontaneous emulsification method and characterized for parameters i.e., physical appearance, physical appearance, droplet size, polydispersity index (PDI), % drug content, viscosity, pH, in-vitro drug release and stability. In results, the H. vittatum flowers were extracted through cold maceration process using ethyl alcohol and percentage yield was found to be 11.29 %. Herbal extract showed the maximum absorption wavelength (λmax) at 290nm when determined in the conc. range of 2 µg/ml to 12 µg/ml. The maximum in-vitro drug release was observed in F3 formulation i.e., 95.26±0.4 (12 hours). After 1 month, the % drug content, pH and in-vitro drug release were re-checked and found significant unchanged. It concluded that the nanoemulsion development of H. vittatum flowers extract enhanced the solubility with the utilization of surfactant Pluronic F127. The minimum droplet size and larger surface area improved the solubility and dispersion of the active bioactive molecules. In suggests, the researchers to determine the mode of action for diverse pharmacological properties thus H. vittatum flowers extract-based nanoemulsion can be utilized in management of various diseases.
Downloads
References
[1] Singh R, Lillard JW. Nanoparticle-based targeted drug delivery. Exp Mol Pathol. 2009;86:215–223.
[2] Mason TG, Wilking JN, Meleson K, et al. Nanoemulsions: formation, structure, and physical properties. J Phys: Condens Matter. 2006;18:R635–R666.
[3] Tan SF, Masoumi HR, Karjiban RA, et al. Ultrasonic emulsification of parenteral valproic acid-loaded nanoemulsion with response surface methodology and evaluation of its stability. Ultrason Sonochem. 2016;29:299–308.
[4] Silva Ana Flavia Schurmann da, Jean Paulo de Andrade, Lia R M Bevilaqua, Márcia Maria de Souza, Ivan Izquierdo, Amelia Teresinha Henriques, Jose Angelo Silveira Zuanazzi. Anxiolytic-, antidepressant- and anticonvulsant-like effects of the alkaloid montanine isolated from Hippeastrum vittatum. Pharmacol Biochem Behav. 2006;85(1):148-54.
[5] Poggio L, Gonzalez G, Naranjo CA. Chromosome studies in Hippeastrum (Amaryllidaceae): variation in genome size. Bot J Linn Soc. 2007; 155(2):171–178.
[6] Robert B, Jordheim M, Kiremire B, Namukobe J, Andersen Om. Anthocyanins from flowers of Hippeastrum cultivars. Sci Hortic-Amsterdam. 2007; 109(3): 262–266.
[7] Hui Ma, Wang Qi, Yuan Yanbo. Germplasm Resources of Hippeastrums spp. and Their Application to Landscaping. World Forestry Research, 2012, 04: 28-33.
[8] Chen Wanqing, Zheng Rongshou, Zeng Hongmei. Report of Cancer Incidence and Mortality in China 2012. China Cancer, 2015, 01: 1-10.
[9] Wang Yong xiang, Xin Guosong, Li Yinglin. Study on the Chemical Composition and Pharmacological Effects of Hippeastrum Vittatum. Heilongjiang Medical Journal, 2015, 02:231-235.
[10] Liu Caiping, Hu Hao, Zhang Yanping. Analysis of malignant tumor death in Yinchuan city in 2012. Journal of Medical Pest Control, 2015, 01: 35-38.
[11] Khan Mohammad Asif, S B Tiwari, H Gupta, Huma Noor, 2020. Evaluation of anxiolytic and antidepressant potential of hydro-alcoholic leaves extract of Azadirachta indica in albino rats. PharmacologyOnline, 3, 207-213.
[12] Khandelwal K R. (2002). Practical Pharnacognosy, Nirali Prakashan, Pune, 9th Edition, 157-158.
[13] Bhatt J. S. and S. Dhyani, (2012). Preliminary Phytochemical Screening of Ailanthus Excelsa Roxb., Int. J. Curr. Pharmaceut. Res., 4(1), 87-89.
[14] Reddy Yella Thirupathi, Rohit Saraswat, Reddy Sunil, 2019. Design and Characterization of Buccal Patches of Atomoxetine. International Journal of Pharmaceutical Sciences Review and Research, 56(1), 96-102.
[15] Salman Akram, Nicolas Anton, Ziad Omran, Thierry Vandamme. Water-in-Oil Nano-Emulsions Prepared by Spontaneous Emulsification: New Insights on the Formulation Process. Pharmaceutics, 2021, 13(7), 1030.
[16] Samadhan K. Deore, Rajendra K Surawase, Avish Maru. Formulation and Evaluation of O/W Nanoemulsion of Ketoconazole. Res. J. Pharma. Dosage Forms and Tech.2019; 11(4):269- 274.
[17] Makhmalzadeh BS, Torabi S, Azarpanah A. Optimization of ibuprofen delivery through rat skin from traditional and novel nanoemulsion formulations. Iran J Pharm Res, 2012;11:47-58.
[18] Derie DV, Burade KB, Kotwal RS, Galkwad VB. Formulation and evaluation of microemulsion based gel for topical delivery of ketoconazole. Indian Drugs, 2008;45:138-40.
[19] Jufri M, Natalia M. Physical stability and antibacterial activity of black cumin oil (Nigella sativa L.) nanoemulsion gel. Int J Pharmtech Res, 2014;6:1162-9.
[20] Dhanshree Panchabudhe, Jitendra Patel, Rukmani Patel. Nanoemulsion Formulation and Evaluation of Terminalia chebula Fruit to Improve Bioavailability. Asian Journal of Pharmaceutics 2025; 19(1): 195.
[21] Verma S, Chauhan S, Dubey M, Verma S, Agrahari D. Formulation and Evaluation of Herbal Nanoemulgel for Anti-inflammatory Activity. Int J Drug Deliv Technol. 2026;16(51s): 1987-2000.
[22] Sumon Giri, Anhic Chakraborty, Chiranjit Mandal, Tushar Kanti Rajwar, Jitu Halder, Zainab Irfan, Mostafa M Gouda. Formulation and Evaluation of Turmeric- and Neem-Based Topical Nanoemulgel against Microbial Infection. Gels. 2024;10(9):578.
[23] Alam M. S, Ali M. S, Alam N, Alam M. I, Anwer T, Imam F, &ShamimM. (2012). Design and characterization of nanostructure topical gel of betamethasone dipropionate for psoriasis. Journal of Applied Pharmaceutical Science, 2(10): Pages 148.
[24] Tatiya A, Bhavsar S, Mahajan H, Surana S. Experimental Design and Characterization of Nanoemulsion Based Topical Herbal Gel Developed for Site-Specific Activity of Glycyrrhiza Glabra Extract: in Vitro And Ex- Vivo Studies. Biosc. Biotech. Res. Comm. 2019;12(2).
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
