Isolation Of Microbes From Dairy Effluent And Treated With Sewage

Authors

  • K. Sivaraman
  • S. Antony
  • S. Antony
  • R. C. Ramya

Keywords:

Dairy effluent; Bacterial isolation; Sewage bioremediation; Physicochemical analysis; HPLC..

Abstract

Dairy industries generate large quantities of wastewater containing high concentrations of organic matter and nutrients, which contribute to environmental pollution if discharged without proper treatment. The present study was carried out to isolate and identify bacterial species from dairy effluent and evaluate their potential in the treatment of sewage. Dairy effluent samples were collected from Milma Industry, Thiruvananthapuram, and bacterial isolates were obtained by serial dilution and culture techniques. The isolates were identified using morphological, Gram staining, biochemical, and physiological characteristics. Among fifty bacterial colonies, twelve isolates were selected and tentatively identified as Lactobacillus, Pseudomonas, Arthrobacter, Escherichia, Bacillus, Microbacterium, Micrococcus, Azotobacter, Vibrio, Pasteurella, Staphylococcus, and Bifidobacterium. Sewage samples were analyzed for physicochemical parameters before and after microbial treatment. The treatment reduced turbidity, salinity, electrical conductivity, total solids, total dissolved solids, and total suspended solids, indicating the biodegradation potential of the selected isolates. HPLC analysis further confirmed the presence of degradation-associated compounds after treatment. The findings demonstrate that indigenous bacterial isolates from dairy effluent can effectively improve sewage quality and may serve as promising candidates for environmentally friendly and cost-effective wastewater bioremediation..

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References

[1] Bharati, S., Shete, A., & Shinkar, N. P. (2013). Dairy industry wastewater: Sources, characteristics and its effects on environment. 3(5).

[2] Briao, V. B., & Tavares, C. R. G. (2007). Effluent generation by the dairy industry: Preventive attitudes and opportunities. Brazilian Journal of Chemical Engineering, 24, 487–497.

[3] Cosa, S., & Okoh, A. I. (2014). Bioflocculant production by a consortium of two bacterial species and its potential application in industrial wastewater and river water treatment. Polish Journal of Environmental Studies, 23, 689–696.

[4] Danalewich, J. R., Papagiannis, T. G., Belyea, R. L., Tumbleson, M. E., & Raskin, L. (1998). Characterization of water treatment of industrial wastewater.

[5] Farizoglu, B., & Uzuner, U. (2011). The investigation of dairy industry wastewater treatment in a biological high-performance membrane system. Biochemical Engineering Journal, 57, 46–54.

[6] Gaikwad, G. L., Wate, S. R., Ramteke, D. S., & Roychoudhury, K. (2014). Development of microbial consortia for the effective treatment of complex wastewater. Journal of Bioremediation & Biodegradation, 5(4).

[7] Irina Schneider, & Topalova, Y. (2010). Bioaugmentative approaches for dairy wastewater treatment. American Journal of Agricultural and Biological Sciences, 5(4), 459–467.

[8] Kelly, P. T., & Zhen, H. (2014). Understanding the application niche of microbial fuel cells in a cheese wastewater treatment process. Bioresource Technology, 157, 154–160.

[9] Kushwaha, J. P., & Srivastava, V. C. (2011). An overview of various technologies for the treatment of dairy wastewaters. Critical Reviews in Food Science and Nutrition, 51, 442–452.

[10] Manh, L. D. (2008). Bioremediation of vegetable oil and grease from polluted wastewater in dairy factory. Journal of Science, Natural Science and Technology, 24, 56–62.

[11] Nasr, M., Elreedy, A., Abdel-Kader, A., Elbarki, W., & Moustafa, M. (2014). Environmental consideration of dairy wastewater treatment using a hybrid sequencing batch reactor. Sustainable Environment Research, 24.

[12] Prakashveni, R., & Jagadeesan, M. (2012). Isolation, identification and distribution of bacteria in dairy effluent. Advances in Applied Science Research, 3(3), 1316–1318.

[13] Porwal, J., Mane, A. V., & Velhal, S. G. (2015). Biodegradation of dairy effluent by using microbial isolates obtained from activated sludge. Water Resources and Industry, 9, 1–15.

[14] Verheijen, L. A. H. M., Wiersema, D., Hulshoff Pol, L. W., & De Wit, J. (1996). Management of waste from animal product processing. International Agricultural Centre.

[15] Vida, M., Akbar, S., & Zahra, G. (2007). Biodegradation of effluents from dairy plant by bacterial isolates. Iranian Journal of Chemical Engineering, 26, 55–59.

[16] Vishakha, S. S., Minal, W., & Khetmalas, M. B. (2013). Isolation of microorganisms from dairy effluent. British Microbiology Research Journal, 3(3), 346–354..

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Published

2025-12-12

How to Cite

1.
K. Sivaraman KS, S. Antony SA, S. Antony SA, Ramya RC. Isolation Of Microbes From Dairy Effluent And Treated With Sewage. J Neonatal Surg [Internet]. 2025 Dec. 12 [cited 2026 Sep. 12];14(33S):1375-84. Available from: https://jneonatalsurg.com/index.php/jns/article/view/10513

Issue

Vol. 14 No. 33S (2025): Journal of Neonatal Surgery

Section

Original Article

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