Free Radicals Scavenging Activity Of Lemon Grass, Mint Herbal Formulation Mediated Zinc Oxide Nanoparticles.
Keywords:
Zinc oxide nanoparticles, Biosynthesis, Herbal formulation, Antioxidant activity, Free radical scavenging, DPPH assay, Lemongrass, Mint...Abstract
BACKGROUND:The traditional synthesis of metal oxide nanoparticles frequently relies on physical and chemical methods that utilize toxic chemicals, which pose potential hazards to human health and the environment. To mitigate these risks, there is a growing shift toward "green chemistry," which utilizes herbal materials to provide eco-friendly, non-toxic alternatives for pharmaceutical and biomedical applications.
OBJECTIVE :To evaluate the in vitro antioxidant and free radical scavenging activity of the formulated biogenic nanoparticles at various concentrations using DPPH and hydroxyl radical (H2O2) scavenging assays.
MATERIALS AND METHOD:This study investigated the biosynthesis of zinc oxide nanoparticles (ZnO-NPs) utilizing a lemongrass and mint herbal formulation. This green synthesis method bypasses the need for toxic chemicals, intricate purification, and microbial cell cultures. The free radical scavenging activity of the synthesized nanoparticles was evaluated in vitro at various concentrations (10 µL to 50 µL) using
- DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay.
- Hydroxyl radical (H2O2) scavenging assay.
RESULTS :The lemongrass and mint herbal formulation successfully and easily mediated the synthesis of ZnO-NPs. In both the DPPH and H2O2 assays, the biogenic nanoparticles demonstrated a strong, dose-dependent ability to neutralize free radicals. This free-radical scavenging activity closely competed with the standard antioxidant controls.
CONLUSION:The biosynthesis of zinc oxide nanoparticles using a lemongrass and mint formulation is a highly effective, non-toxic, and environmentally compatible alternative to conventional chemical synthesis. Due to their potent antioxidant capabilities and the simplicity of the plant-based procedure, these biogenic nanoparticles hold significant potential for scalable production and use in pharmaceutical and commercial medicinal applications to counteract oxidative stress.
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