Genetic Association Of Aurora Kinase Gene (Aukra T>A) Polymorphism With Oscc

Authors

  • Ashwin Krishna B
  • Karthikeyan Murthykumar

Keywords:

Aurora Kinase A (AURKA), Gene Polymorphism, Single Nucleotide Polymorphism (SNP), Oral Squamous Cell Carcinoma (OSCC), rs2273535, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) , Genetic Susceptibility

Abstract

Background: Aurora kinase A (AURKA) is a serine/threonine kinase that plays a pivotal role in mitotic spindle assembly, centrosome maturation, and chromosomal segregation. Disruption or upregulation of AURKA checkpoints can induce genetic instability and aneuploidy, serving as a critical driver in tumorigenesis. This study aimed to investigate the genetic association between the AURKA gene (T>A) polymorphism (rs2273535) and Oral Squamous Cell Carcinoma (OSCC) in a South Indian population. 

Materials and Methods: A cross-sectional study was conducted utilizing venous blood samples from 100 participants partitioned into clinical groups, including a group of cases and periodontally healthy controls (n=25 per evaluated sub-cohort). Genomic DNA was isolated using a modified Miller's protocol. Polymerase Chain Reaction (PCR) amplification was executed using custom primers spanning the polymorphic site, yielding a 165 bp amplicon, followed by restriction fragment length polymorphism (RFLP) digestion utilizing the ApoI endonuclease. Genotype frequencies, allele distributions, and Hardy-Weinberg Equilibrium (HWE) compliance were statistically analyzed using Chi-Square tests and Odds Ratios (OR) via SPSS software.  Results: The distribution of the AURKA rs2273535 polymorphism conformed to HWE in both cohorts (P > 0.05). Genotype frequencies for the case group were 0\% for AA, 52\% (n=13) for AT, and 48\% (n=12) for TT. For the control group, frequencies were 0\% for AA, 60\% (n=15) for AT, and 40\% (n=10) for TT. Statistical evaluation revealed no significant variation in overall genotype distribution between cases and controls (\chi^2, P = 0.5688). Furthermore, allele frequency shifts (T vs. A) showed no statistically significant correlation with elevated risk profile under dominant (P = 1.0000), recessive (P = 0.5693), or individual allelic (P = 0.6562) models. Conclusion: The findings demonstrate that the AURKA (rs2273535) gene polymorphism is not significantly associated with a susceptibility to oral lesions within the evaluated sample size. While AURKA aberrations remain a critical hallmark in broad human malignancies, larger multi-ethnic studies with expanded cohorts are required to fully elucidate its explicit synergistic impact on OSCC risk progression...

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Published

2024-09-07

How to Cite

1.
Krishna B A, Murthykumar K. Genetic Association Of Aurora Kinase Gene (Aukra T>A) Polymorphism With Oscc. J Neonatal Surg [Internet]. 2024 Sep. 7 [cited 2026 Jul. 2];13(1):2425-30. Available from: https://jneonatalsurg.com/index.php/jns/article/view/10373

Issue

Vol. 13 (2024): Journal of Neonatal Surgery

Section

Original Article

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