“Evaluation of ayurvedic detoxification process (Shodhana) of Strychnos nux vomica linn seeds w. s. r. to its LD50”.
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N\AAbstract
In Ayurveda a very useful procedure of Shodhana Sanskar i.e. detoxification process is mentioned. It helps not only to remove impurities but also is useful to enhance required/favourable properties of the poison and avoid the harmful ones, by which we can confidently state the safety of the drug prepared from the vishadravyas (poisonous drug). Here the attempts were made to study the changes in phytochemical properties and LD50 values of Strychnos nux vomica seed powder due to detoxification process (shodhana sanskar). LD50 is frequently used as a general indication of substance acute toxicity. As the signs and symptoms of toxicity of nux vomica seed is seen immediately after consumption. Hence, acute toxicity study has been chosen.
Objectives: To evaluate the effects of shodhana process i.e. detoxification process on toxicity level of Strychnos nux vomica seeds.
Material and methods: Detoxification (Shodhana) of Strychnos nux vomica seeds was done by method mentioned in Ayurvedic text Rasatarangini. The phytochemical analysis was performed for both before and after detoxification (shodhana) samples. Also, acute toxicity study i.e. LD 50 study was performed for both samples by following OECD Guidelines for Testing Chemicals.
Results: Strychnine value was reduced by 1.02% in after detoxification sample.
Before detoxification (shodhana) process LD50 value is found to be less than 300 mg/kg but more than 50 mg/kg body weight in albino mice by oral route. After detoxification (shodhana) process LD50 value is greater than 2000 mg/kg body weight in albino mice by oral route.
Conclusion: Detoxification process (Shodhana) affects phyto-chemical properties of Strychnos nux vomica seed powder. After the process of detoxification, the quantity of main phytochemical constituents strychnine and brucine are found to be reduced on HPTLC.
Detoxification (Shodhana) process increases the value of LD50 of nux vomica seed powder. i.e. it reduces the toxicity of Strychnos nux vomica seed powder. .
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References
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