Anticariogenic Activity of Avocado Seed Extract Mediated Synthesis of Selenium Nanoparticle.
Keywords:
selenium nanoparticles, avocado seed, green synthesis, anti cancer activityAbstract
Background: Dental caries is a multifactorial infectious disease resulting from the interaction between cariogenic bacteria, fermentable carbohydrates, and host factors. Aim: The aim of the study is to study the Anticariogenic Activity of Avocado Seed Extract Mediated Synthesis of Selenium Nanoparticle. Method:Fresh avocado fruits were collected and the seeds were separated, washed thoroughly, and air-dried. The dried seeds were powdered using a mechanical grinder. Approximately 10 g of seed powder was mixed with 100 mL of distilled water and heated at 60–70°C for 20 minutes. The mixture was filtered using Whatman No.1 filter paper to obtain a clear extract, which was stored at 4°C for further use. Results: The synthesized selenium nanoparticles exhibited measurable antimicrobial activity against all tested organisms. A concentration-dependent increase in inhibition was observed particularly against Staphylococcus aureus and Candida albicans. The highest inhibition was observed against Candida albicans at 100 µL with a zone of 13 mm. However, comparatively lower activity was observed against Enterococcus faecalis and Streptococcus mutans. Conclusion: This study demonstrates that selenium nanoparticles can be successfully synthesized using avocado seed extract through a green and eco-friendly method. The synthesized nanoparticles exhibited antimicrobial activity against important oral pathogens including Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, and Candida albicans..
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