Phytochemical Profiling and Quantification of Premna herbacea Roxb. Extracts Using Successive Solvent Extraction.

Authors

  • Arati Kapase
  • Swapnil Goyal
  • Ansar Patel

Keywords:

Premna herbacea, Phytochemical profiling, Total phenolic content, Total flavonoid content, Successive solvent extraction

Abstract

Premna herbacea Roxb  is an unconventional accepted medicinal plant with a long-standing therapeutic potential known to have; however, a scientific validation of the phytochemical composition is limited. The aim of the investigation carried out was to determine the effect of polarity of solvents on phytochemical extraction, as well as, determine the best solvent system to be used to extract maximum bioactive compounds. Solvents with petroleum ether, chloroform, ethyl acetate, and ethanol were consecutively used to extract solvents. Qualitative phytochemical screening indicated a notable solvent dependent differences in the phytochemical profiles with non-polar extracts having fewer constituents compared to polar extracts which have increased diversity. Quantitative analysis proved that the ethanolic extract had the most total phenolic content (112.783.54mg GAE/g extract) and total flavonoid content (86.430.67mg QE/g extract), followed by the ethyl acetate, chloroform and petroleum ether extracts. It was evident that the solvent polarity and the phytochemical yield are correlated with each other indicating that polar solvents are superior in extracting antioxidant-rich compounds. The ethanolic extract was also found to have a better aptitude to nanoformulation development with phytochemical abundance and compatibility with formulation. The research provides the scientific basis of ethanol as the most efficient extraction agent and also it provides a strong phytochemical basis on the pharmaceutical use of P. herbacea especially in antioxidant and formulation related research..

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Published

2022-12-07

How to Cite

1.
Kapase A, Goyal S, Patel A. Phytochemical Profiling and Quantification of Premna herbacea Roxb. Extracts Using Successive Solvent Extraction. J Neonatal Surg [Internet]. 2022 Dec. 7 [cited 2026 Apr. 1];11:65-74. Available from: https://jneonatalsurg.com/index.php/jns/article/view/10119

Issue

Vol. 11 (2022): Journal of Neonatal Surgery

Section

Original Article

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