Protective Role of Hygrophila auriculata Seed Extract Against Carbon Tetrachloride Induced Hepatocellular Damage: An In Vitro Study

Authors

  • Sowmyashree G
  • Vyshali P
  • Thilagavathy A
  • Thejaswi Bhandary

Keywords:

Hepatic Injury, Cytotoxicity, Hepatoprotection, CCL4, Anti-Oxidant Property

Abstract

Hepatic injury induced by xenobiotics is closely associated with oxidative stress–mediated cellular damage. The present study evaluated the phytochemical composition, antioxidant activity, and hepatoprotective efficacy of the aqueous seed extract of Hygrophila auriculata using a carbon tetrachloride (CCl₄) induced goat liver slice model. Preliminary screening confirmed the presence of flavonoids, saponins, tannins, and proteins. Quantitative estimation revealed abundant protein (231 ± 10.06 mg BSAE/g), substantial total phenolics (187 ± 20.29 mg GAE/g), and notable flavonoids (62.09 ± 7.23 mg QE/g). The extract exhibited a concentration-dependent DPPH radical scavenging effect with an IC₅₀ of approximately 72–73 µg/mL, indicating moderate yet significant antioxidant potential. Whereas, CCl₄ exposure (15 mM) markedly elevated SGOT, SGPT, and LDH activities (p < 0.001), reflecting hepatocellular membrane disruption. Post-treatment with the extract (0.02–0.10 mg QE/mL) significantly attenuated enzyme leakage in a dose-responsive manner (p < 0.05 vs. CCl₄). At 0.10 mg QE/mL, enzyme levels declined by 59.9% (SGOT), 47.3% (SGPT), and 31.7% (LDH), with corresponding hepatoprotection values of 71.7%, 62.0%, and 51.0%. The protective effect approached that of quercetin. These findings suggest that the polyphenol-rich seed extract confers hepatoprotection primarily through antioxidant-mediated mechanisms

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References

World Health Organization. WHO global report on traditional and complementary medicine 2019. Geneva: WHO; 2019.

2. Hussain A, Wahab S, Zarin I, Hussain MD. Antibacterial activity of Hygrophila auriculata. Afr J Biotechnol. 2009;8(15):3378–3382. https://doi.org/10.5897/AJB09.225

3. Sethiya NK. Hygrophila auriculata: A review of phytochemistry and pharmacology. Pharmacogn Rev. 2018;12(23):1–9. https://doi.org/10.4103/phrev.phrev_33_17

4. Rastogi S, et al. Antioxidant activity of Hygrophila auriculata seed extracts. J Appl Pharm Sci. 2014;4(3):50–55. https://doi.org/10.7324/JAPS.2014.40309

5. Finkel T, Holbrook NJ. Oxidants, oxidative stress and ageing. Nature. 2000;408:239–247. https://doi.org/10.1038/35041687

6. Holt MP, Ju C. Mechanisms of drug-induced liver injury. AAPS J. 2006;8(1):E48–E54. https://doi.org/10.1208/aapsj080106

7. Manibusan MK, Odin M, Eastmond DA. Postulated carbon tetrachloride mode of action: A review. J Environ Sci Health C. 2007;25(3):185–209. https://doi.org/10.1080/10590500701569398

8. Li S, Tan HY, Wang N, Zhang ZJ, Feng Y. The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci. 2015;16(11):26087–26124. https://doi.org/10.3390/ijms161125942

9. Harborne JB. Phytochemical Methods. London: Chapman & Hall; 1973.

10. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques. 3rd ed. London: Chapman & Hall; 1998.

11. Mojab F, Kamalinejad M, Ghaderi N, Vahidipour HR. Phytochemical screening of some Iranian plants. Iran J Pharm Res. 2003;2:77–82.

12. Auwal MS, et al. Phytochemical screening techniques. J Pharm Biol Sci. 2014;9(3):77–81.

13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–275. https://doi.org/10.1016/S0021-9258(19)52451-6

14. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content by aluminum chloride colorimetric assay. J Food Drug Anal. 2002;10(3):178–182.

15. Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant activity of flavonoids from Licania licaniaeflora. J Nat Prod. 2002;65:611–615. https://doi.org/10.1021/np0100845

16. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28:56–63. https://doi.org/10.1093/ajcp/28.1.56

17. King J. Practical Clinical Enzymology. London: Van Nostrand; 1965.

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Published

2025-03-07

How to Cite

1.
Sowmyashree G SG, Vyshali P VP, Thilagavathy A TA, Bhandary T. Protective Role of Hygrophila auriculata Seed Extract Against Carbon Tetrachloride Induced Hepatocellular Damage: An In Vitro Study. J Neonatal Surg [Internet]. 2025 Mar. 7 [cited 2026 May 22];14(3):402-11. Available from: https://jneonatalsurg.com/index.php/jns/article/view/10103

Issue

Vol. 14 No. 3 (2025): Journal of Neonatal Surgery

Section

Original Article

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