Eco-friendly HPTLC method for simultaneous estimation of Quercetin and Gallic Acid in marketed herbal gel(kiwi) formulation
DOI:
https://doi.org/10.63682/jns.v14i33S.10032Keywords:
Eco-friendly HPTLC, Gallic Acid, Green Analytical Chemistry, Kiwi Herbal Gel, QuercetinAbstract
The analysis of herbal formulations is often complicated by complex plant matrices and the environmental impact of analytical waste. This study presents a green High-Performance Thin-Layer Chromatography (HPTLC) method for the simultaneous estimation of Quercetin and Gallic Acid in a marketed kiwi (Actinidia deliciosa) herbal gel. To align with Green Analytical Chemistry (GAC) principles, an eco-friendly mobile phase of Toluene, Ethyl Acetate, and Formic Acid (5:4:1 v/v/v) was developed, replacing more hazardous solvent systems. The optimized method achieved superior chromatographic resolution with sharp peaks and reproducible Rf values. Validation was conducted per ICH Q2(R1) guidelines, confirming high sensitivity (LOD/LOQ), linearity, accuracy, and robustness. Additionally, the method's sustainability was verified using the AGREE (Analytical GREEnness) metric. This HPTLC approach offers a rapid, cost-effective, and environmentally responsible solution for the routine quality control and standardization of multi-component herbal topical formulations
Downloads
References
[1] World Health Organization. WHO guidelines on safety monitoring of herbal medicines in pharmacovigilance systems. Geneva: World Health Organization; 2004.
[2] Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014;4:177. doi:10.3389/fphar.2013.00177
[3] Shah M, Patel HU, Akabari AH. Eco-friendly HPTLC method for simultaneous estimation of gallic acid, ellagic acid, and curcumin biomarker in herbal formulation. Essential Chem. 2024;1(1):1-10. doi:10.1080/28378083.2024.2420104
[4] Pena-Pereira F, Wojnowski W, Tobiszewski M. AGREE—Analytical GREEnness Metric Approach and Software. Anal Chem. 2020;92(14):10076-82. doi:10.1021/acs.analchem.0c01887
[5] Parasuraman S, Thing GS, Dhanaraj SA. Enhanced liver functions following herbal treatment with Achyranthes aspera in albino rats. J Nat Remedies. 2013;13(2):129-36.
[6] Bhuiyan MA. Quality control and standardization of herbal drugs. In: Khan MTH, Ather A, editors. Leading edge herbs and herbal extracts: therapeutic and technological advances. New York: Nova Science Publishers; 2007. p. 25-48.
[7] Bauer R. Quality control and standardization of herbal drugs. In: Mukherjee PK, editor. Quality control and evaluation of herbal drugs. 1st ed. New Delhi: Business Horizons Pharmaceuticals; 2010. p. 43-58.
[8] Kunle OF, Egharevba HO, Ahmadu PO. Standardization of herbal medicines - a review. Int J Biodivers Conserv. 2012;4(3):101-12. doi:10.5897/IJBC11.163
[9] Gauri M, Daswani P, Vora J, Kshirsagar N, Rajani M. Standardisation of polyherbal formulations. Arch Appl Sci Res. 2011;3(5):382-94.
[10] Choudhary N, Sekhon BS. An overview of advances in the standardization of herbal drugs. J Pharm Educ Res. 2011;2(1):55-65.
[11] Atanasov AG, Zotchev SB, Dirsch VM, Supuran CT. Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discov. 2021;20(3):200-16. doi:10.1038/s41573-020-00114-z
[12] Rasheed A, Roja C, Kumar A, Reddy BS. Formulation, standardisation and pharmacological evaluation of a polyherbal traditional remedy. Orient Pharm Exp Med. 2012;12(1):51-8. doi:10.1007/s13596-011-0053-1
[13] U.S. Food and Drug Administration. Botanical drug development: guidance for industry. Silver Spring, MD: U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER); 2016.
[14] Yadav NP, Dixit VK. Recent advances in traditional plant drugs. Indian J Pharm Sci. 2008;70(4):427-33. doi:10.4103/0250-474X.45908
[15] Nikam P, Kareparamban J, Jadhav A, Kadam V. Future trends in standardization of herbal drugs. J Pharmacogn Phytochem. 2013;2(3):24-31.
[16] Reich E, Schibli A. High-performance thin-layer chromatography for the analysis of medicinal plants. New York: Thieme; 2007.
[17] Attimarad M, Momin SAM, Umar A, Aly AM. HPTLC: An important tool in modern pharmaceutical analysis. J Pharm Bioallied Sci. 2012;4(Suppl 2):S98. doi:10.4103/0975-7406.94895
[18] Patil PS, Gulkari VD, Patil JS, Nalawade SS, Dhabale PN. Development and validation of HPTLC method for herbal gel formulation. J Appl Pharm Sci. 2012;2(10):34-9. doi:10.7324/JAPS.2012.21007
[19] International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH). Validation of analytical procedures: text and methodology Q2(R1). Geneva: ICH; 2005.
[20] European Medicines Agency. Guideline on quality of herbal medicinal products/traditional herbal medicinal products. London: EMA; 2018. (EMA/CPMP/QWP/2819/00 Rev. 2 Corr.).
[21] Bonde SD, Jat R, Patel M. Simultaneous marker-based standardization of gel by HPTLC. J Pharmacogn Phytochem. 2019;8(3):543-7.
[22] Patil PM, Wankhede SB, Chaudhari PD. A validated stability-indicating HPTLC method for estimation of acyclovir in tablets in presence of its alkaline hydrolysis degradation product. Bull Fac Pharm Cairo Univ. 2014;52(2):245-57. doi:10.1016/j.bfopcu.2014.09.002
[23] Wang Y, Li L, Liu H, Zhao T, Meng C, Liu Z, et al. Bioactive compounds and antioxidant activities of kiwi fruit. Int J Food Sci Technol. 2018;53(9):2239-45. doi:10.1111/ijfs.13812
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
