Prevention of Histological Changes after Colonic Diversion in Rats: An Experimental Study

M Pathak; M Srinivas; A Shariff

doi:10.21699/jns.v6i2.511

Prevention of Histological Changes after Colonic Diversion in Rats: An Experimental Study

Authors

M Pathak All India Institute of Medical sciences, Jodhpur
M Srinivas
A Shariff

DOI:

https://doi.org/10.21699/jns.v6i2.511

Keywords:

Histology, Colonic diversion, Glutamine, Psyllium, Short Chain Fatty Acids, Maharishi Amrit Kalash, Enema

Abstract

AIM: To determine the beneficial effects of Glutamine, Psyllium, Short Chain Fatty Acids (SCFA), and Maharishi Amrit Kalash (MAK), in preventing the histological changes after diversion colostomy.

MATERIAL AND METHODS: After ethical clearance, male wistar rats (n=40) underwent diversion colostomy. Rats were divided into five groups of 8 rats each. Each group was given, after diversion colostomy, per rectally, one of the five agents being tested as an enema (3 ml/kg/day). Group I: Normal saline. Group II: Glutamine Group III: Psyllium, Group IV: MAK. Group V: Short chain fatty acid. The rats were euthanised 45 days after performing diversion colostomy. Morphometrical analysis of defunctionalised colon was done. Statistical analysis was done using SSPS statistical analysis software.

RESULTS: On comparison with Group I epithelial cell height and mucosal thickness was significantly higher in Group II. Muscularis externae thickness was significantly higher in Group III on comparison with Group I. Group V had least inflammatory changes. CONCLUSIONS: Atrophic and inflammatory changes in the diverted colon can be prevented by per rectal administration of Glutamine, Psyllium and Short chain fatty acids.

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Author Biography

M Pathak, All India Institute of Medical sciences, Jodhpur

Associate Professor,

Department of Pediatric surgery,

AIIMS, Jodhpur

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Published

2017-04-15

How to Cite

Pathak M, Srinivas M, Shariff A. Prevention of Histological Changes after Colonic Diversion in Rats: An Experimental Study. J Neonatal Surg [Internet]. 2017Apr.15 [cited 2024Dec.14];6(2):26. Available from: https://jneonatalsurg.com/ojs/index.php/jns/article/view/481

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Issue

Vol. 6 No. 2 (2017): Journal of Neonatal Surgery

Section

Original Article

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